Biotechnology Journal,
Journal Year:
2024,
Volume and Issue:
19(4)
Published: April 1, 2024
Due
to
their
high-quality
characteristics,
Chinese
hamster
ovary
(CHO)
cells
have
become
the
most
widely
used
and
reliable
host
for
production
of
recombinant
therapeutic
proteins
in
biomedical
field.
Previous
studies
shown
that
m6A
reader
YTHDF3,
which
contains
YTH
domain,
can
affect
a
variety
biological
processes
by
regulating
translation
stability
target
mRNAs.
This
study
investigates
effect
YTHDF3
on
transgenic
CHO
cells.
The
results
indicate
stable
overexpression
significantly
enhances
protein
expression
without
affecting
cell
growth.
Transcriptome
sequencing
indicated
several
genes,
including
initiation
factor,
extension
ribosome
assembly
were
upregulated
overexpressing
YTHDF3.
In
addition,
cycloheximide
experiments
confirmed
enhanced
transgene
promoting
conclusion,
findings
this
provide
novel
approach
mammalian
engineering
increase
productivity
m6A.
Frontiers in Oncology,
Journal Year:
2025,
Volume and Issue:
15
Published: May 8, 2025
RNA
N
6-methyladenosine
(m6A)
readers
mediate
cancer
progression.
However,
the
role
of
eiptranscriptomic
modifications
such
as
m6A
in
regulation
TNBC
progression
is
unclear.
High-throughput
library
screening
identifies
key
regulator
YTHDF3
TNBC.
Cell
and
animal
experiments
were
used
to
identify
that
promoted
tumorigenesis
enhance
Centromere
protein
I
(CENPI)
translation
via
modification.
We
showed
reader
was
an
independent
risk
factor
associated
with
poor
prognosis
patients.
Notedly,
overexpression
modification,
while
knockdown
markedly
inhibited
proliferation
migratory
ability
tumor
cells
vitro
vivo.
Mechanistically,
interacted
mRNAs
prolong
stability
m6A-modified
RNA.
Our
findings
indicated
contributed
prognosis,
providing
a
potential
prognostic
biomarker
therapeutic
target
for
International Journal of Oncology,
Journal Year:
2023,
Volume and Issue:
64(2)
Published: Dec. 19, 2023
RNA
methylation
modifications
are
closely
linked
to
tumor
development,
migration,
invasion
and
responses
various
therapies.
Recent
studies
have
shown
notable
advancements
regarding
the
roles
of
in
immunotherapy,
microenvironment
metabolic
reprogramming.
However,
research
on
association
between
chemoresistance
N6‑methyladenosine
(m6A)
methyltransferases
specific
cancer
types
is
still
scarce.
Colorectal
(CRC)
among
most
common
gastrointestinal
cancers
worldwide.
Conventional
chemotherapy
remains
predominant
treatment
modality
for
CRC
resistance
primary
cause
failure.
The
expression
levels
m6A
methyltransferases,
including
methyltransferase‑like
3
(METTL3),
METTL14
METTL16,
tissue
samples
associated
with
patients'
clinical
outcomes
efficacy.
Natural
pharmaceutical
ingredients,
such
as
quercetin,
potential
act
METTL3
inhibitors
combat
patients
CRC.
present
review
discussed
different
key
enzymes
development
CRC,
focusing
mechanisms
resistance.
progress
certain
also
listed.
using
natural
remedies
develop
antitumor
medications
that
target
outlined.
Biotechnology Journal,
Journal Year:
2024,
Volume and Issue:
19(4)
Published: April 1, 2024
Due
to
their
high-quality
characteristics,
Chinese
hamster
ovary
(CHO)
cells
have
become
the
most
widely
used
and
reliable
host
for
production
of
recombinant
therapeutic
proteins
in
biomedical
field.
Previous
studies
shown
that
m6A
reader
YTHDF3,
which
contains
YTH
domain,
can
affect
a
variety
biological
processes
by
regulating
translation
stability
target
mRNAs.
This
study
investigates
effect
YTHDF3
on
transgenic
CHO
cells.
The
results
indicate
stable
overexpression
significantly
enhances
protein
expression
without
affecting
cell
growth.
Transcriptome
sequencing
indicated
several
genes,
including
initiation
factor,
extension
ribosome
assembly
were
upregulated
overexpressing
YTHDF3.
In
addition,
cycloheximide
experiments
confirmed
enhanced
transgene
promoting
conclusion,
findings
this
provide
novel
approach
mammalian
engineering
increase
productivity
m6A.
