Probiotics and Antimicrobial Proteins, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 30, 2024
Language: Английский
Cancer Medicine, Journal Year: 2025, Volume and Issue: 14(9)
Published: May 1, 2025
ABSTRACT Background The relationship between hepatocellular carcinoma (HCC) and gut microbiota has gained attention for its impact on HCC immunotherapy. Methods Key microbial metabolites, including bile acids, toll‐like receptor 4, short‐chain fatty bacterial toxins, contribute to progression influence immune responses through the gut‐liver axis. As checkpoint inhibitors (ICIs) become common in treatment, modulating offers new strategies enhance ICIs efficacy. However, individual differences composition introduce challenges, with some patients showing resistance ICIs. Results This review summarizes latest findings role of explores emerging therapeutic approaches, fecal transplantation, probiotics, antibiotics, natural compounds. Conclusions focus is translating these insights into personalized medicine optimize improve treatment outcomes.
Language: Английский
Citations
0
PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17583 - e17583
Published: June 26, 2024
Background Recent studies suggest that gut microbiota composition, abundance and diversity can influence many chronic diseases such as type 2 diabetes. Modulating through targeted nutrition provide beneficial effects leading to the concept of personalized for health improvement. In this prospective clinical trial, we evaluated impact a microbiome-based diet on hyperglycaemic hyperlipidaemic individuals. Specifically, BugSpeaks ® -a microbiome profile test profiles using next generation sequencing provides nutritional recommendation based individual was evaluated. Methods A total 30 participants with diabetes hyperlipidaemia were recruited study. The 15 (test arm) whole genome shotgun metagenomics recommendations their provided. remaining (control provided diabetic guidance 3 months. Clinical anthropometric parameters HbA1c, systolic/diastolic pressure, c-reactive protein levels composition measured compared during Results arm (microbiome-based nutrition) showed statistically significant decrease in HbA1c level from 8.30 (95% confidence interval (CI), [7.74–8.85]) 6.67 CI [6.2–7.05]), p < 0.001 after 90 days. also 5% decline systolic pressure whereas control 7% increase. Incidentally, sub-cohort patients >130 mm Hg by 14%. Interestingly, CRP found drop 19.5%. Alpha measures increase Shannon measure ( 0.05), dietary intervention. intervention led minimum two-fold (Log2 fold change species like Phascolarctobacterium succinatutens, Bifidobacterium angulatum, Levilactobacillus brevis which might have role current context similar Alistipes finegoldii, Sutterella faecalis been earlier shown some negative host. Overall, study indicated net positive regime correlated parameters.
Language: Английский
Citations
3
Folia Microbiologica, Journal Year: 2023, Volume and Issue: 69(2), P. 259 - 282
Published: Dec. 14, 2023
Language: Английский
Citations
7
Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15
Published: Dec. 13, 2024
In contemporary microbial research, the exploration of interactions between microorganisms and multicellular hosts constitutes a burgeoning field. The gut microbiota is increasingly acknowledged as pivotal contributor to various disorders within endocrine system, encompassing conditions such diabetes thyroid diseases. A surge in research activities has been witnessed recent years, elucidating intricate interplay system. Simultaneously, fecal transplantation (FMT) emerged focal point, garnering substantial attention both biomedical clinical spheres. Research endeavors have uncovered remarkable therapeutic efficacy FMT across diverse diseases, with particular emphasis on its application addressing type 2 mellitus (T2DM) associated com-plications. Consequently, this manuscript accentuates intimate connection specific focus exploring potential an intervention landscape T2DM complications. Furthermore, article scrutinizes concerns inherent treatment modalities centered around microbiota, proposing viable solutions address these issues.
Language: Английский
Citations
2
Environmental Toxicology, Journal Year: 2024, Volume and Issue: unknown
Published: Feb. 16, 2024
RNA m6 methyladenosine (m6A) modifications impact tumor biology and immune processes, particularly in hepatocellular malignant tumors. Using a consensus clustering algorithm on 371 carcinoma (HCC) samples, we identified three m6A-modified subtypes correlated them with positive microenvironment (TME) markers for distinct phenotypes. Stratifying patients based m6A scores revealed low presentation group better penetration, lower mutation load, increased expression of checkpoint like CTLA-4 PD-1, suggesting enhanced responsiveness to immunization therapy. A machine-learning model 23 genes was constructed. Single-cell analysis surprising enrichment IGFBP3 astrocytes, prompting the exploration associated signaling pathways. Experimental verification shows that is significantly normal tissues, while immunohistochemical its indicating protective effect HCC good prognosis. Importantly, high outcomes receiving immunotherapy. Moreover, cytotoxic T lymphocyte (CTL) experiments have confirmed stronger cell-killing ability. In summary, comprehensive evaluation modification, characteristics, single-cell this study not only TME but also made significant contributions progress personalized immunotherapy targeting IGFBP3. This provides solid theoretical foundation clinical translation emphasizes potential developing effective treatment strategies.
Language: Английский
Citations
1
Environmental Toxicology, Journal Year: 2024, Volume and Issue: unknown
Published: April 10, 2024
Cervical squamous cell carcinoma (CESC), one of the most common malignancies in women, imposes a significant burden on women's health worldwide. Despite extensive research, molecular and pathogenic mechanisms cervical CESC remain unclear. This study aimed to explore immune-related genes, immune microenvironment infiltration, prognosis CESC, providing theoretical basis for guiding clinical treatment. Initially, by mining four gene sets from public databases, 14 genes associated with were identified. Through univariate multivariate COX regression analyses, as well lasso analysis, CESC-independent prognostic identified, model was constructed, dividing them into high low-risk groups. The correlation between these cells functions explored through ssGSEA enrichment revealing close association high-risk group processes such angiogenesis epithelial-mesenchymal transition. Furthermore, using databases qRT-PCR experiments, differences CXCL8 expression normal cancer discovered. Subsequently, knockdown plasmid efficiency validated two lines, MEG-01 HCE-1. CCK-8, scratch, Transwell assays, it confirmed that could inhibit proliferation, invasion, migration abilities cells. Targeting holds promise personalized therapy strong achieving translation.
Language: Английский
Citations
1
Biology, Journal Year: 2024, Volume and Issue: 13(8), P. 623 - 623
Published: Aug. 15, 2024
Sea urchins play an important role in marine ecosystems. Owing to limitations previous research methods, there has been insufficient understanding of the food sources and ecological functional value purple sea urchins, leading considerable controversy regarding their positioning. We focused on Daya Bay as area, utilizing stable isotope technology high-throughput sequencing 16S rDNA 18S analyze potential stone algae areas. The results showed that δ13C range area is −11.42~−8.17‰, δ15N 9.15~10.31‰. However, algal −13.97~−12.44‰, 8.75~10.14‰. There was a significant difference between two areas (p < 0.05), but no > 0.05). main source for both sediment. revealed that, highest proportion urchin gut Molluska (57.37%). In Arthropoda (76.71%). Bacteroidetes dominant group (28.87%), whereas, Proteobacteria (37.83%). Diversity detection number microbes eukaryotes sediment, organic nutritional greater richness microbiota greater. These indicated are likely omnivores where they occur impacts growth development. this study provide theoretical basis restoration wild resources selection restocking release.
Language: Английский
Citations
1
PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0313548 - e0313548
Published: Nov. 12, 2024
Hepatic complications are the major health issues associated with dietary intake of calorie saturated food e.g. high-fat diet (HFD). Recent studies have revealed beneficial effects probiotics in HFD fed mice hepatic complications. Some probiotic Lactic acid bacteria (LAB) Lactobacillus plantarum drawn our attention managing Here, we aim to elucidate protective L . KAD strain, isolated from ethnic fermented ‘Kinema’ HFD-fed as, a preventive approach. Eighteen Swiss albino were equally divided into 3 groups: Normal Diet (ND), negative control (HFD), and oral supplementation (LP). All experimental groups subjected specific according grouping for eight weeks. After completion regime, subjects anesthetized sacrificed. Organs, blood, fecal samples collected stored appropriately. Physical indices, including body weight gain, organ co-efficients calculated along assessment glycemic, lipidomic, hepatic, oxidative stress, inflammatory, histological parameters. Gut microbiota analysis was performed using 16s V3-V4 metagenomic profiling, sequencing done Illumina Miseq system. Oral administration is found significantly ( p <0.05) restore metabolic by normalizing parameters, stress inflammatory Moreover, LP group (7.08±0.52 mg/g) showed <0.001) decreased triglyceride level compared (20.07±1.32 mg/g). improved adipocytic, colonic histomorphology better scoring pattern. (1.83±0.41) reduced score (5.00±0.63), showing hepatosteatosis, immune infiltration. The strain modulated gut altering its microbial composition positively towards normalization. In conclusion , results experiment suggest that prophylactic has on onset induced mice. Further needed, this clinical use as supplement.
Language: Английский
Citations
1
medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Dec. 28, 2023
Abstract Recent studies suggest that gut microbiota composition, abundance and diversity can influence many chronic diseases such as type 2 diabetes. Modulating through targeted nutrition provide beneficial effects leading to the concept of personalized for health improvement. In this prospective clinical trial, we evaluated impact a microbiome-based diet on hyperglycaemic hyperlipidaemic individuals. Specifically, BugSpeaks ® - microbiome profile test profiles using next generation sequencing provides nutritional recommendation based individual was evaluated. The arm [microbiome-based nutrition] showed statistically significant decrease in HbA1c level [from 8.30 ± 1.12 6.67 0.89, p<0.001] after 90 days. arm, also 5% decline systolic pressure whereas control 7% increase. Incidentally, sub-cohort patients with >130mm Hg by 14%. Interestingly, CRP found drop 19.5%. Alpha measures increase Shannon measure [p<0.05], dietary intervention. intervention led minimum 2-fold [Log2 fold change] species like Phascolarctobacterium succinatutens, Bifidobacterium angulatum, Levilactobacillus brevis , similar non-beneficial Alistipes finegoldii, Sutterella faecalis . Overall, study indicated net positive regime correlated parameters.
Language: Английский
Citations
2
Animal Bioscience, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 26, 2024
Probiotics are living microorganisms that can provide health benefits when consumed. Here, we investigated the effects of probiotics on gene expression in spleen mice using RNA-sequencing analysis between negative control and probiotic groups (including 4 Lactobacillus strains: fermentum, L. casei, plantarum, brevis).
Language: Английский
Citations
0