Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15
Published: Nov. 21, 2024
Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal malignancy with poor prognosis. Epigenetic dysregulation plays crucial role in PDAC progression, but its comprehensive landscape and clinical implications remain unclear.
Language: Английский
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: Sept. 11, 2023
Regulatory T cells (Treg), as members of CD4+ cells, have garnered extensive attention in the research tumor progression. Treg function inhibiting immune effector preventing tissue damage, and suppressing inflammation. Under stimulation inflammatory microenvironment (IM), reprogramming enhances their suppression responses, ultimately promoting escape or Reducing number IM lowering activity while reprogramming, can help promote body's anti-tumor responses. This review introduces a mechanism IM; discusses regulation on The control response to immunotherapy are analyzed countermeasures proposed. work will provide foundation for downregulating immunosuppressive role environment future immunotherapy.
Language: Английский
Citations
37
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: March 14, 2025
Colorectal cancer (CRC) is one of the most prevalent malignancies worldwide, with high postoperative recurrence and metastasis rates posing significant challenges to patient survival. Identifying reliable accessible prognostic markers essential for optimizing treatment strategies. This study investigates significance two preoperative hematological indices, [neutrophils × platelets]/[lymphocytes hemoglobin] (NP/LHb) ratio absolute monocyte count (Mono), in predicting overall survival CRC patients. A retrospective analysis 566 patients was conducted, cohort serving as an external validation set. Receiver operating characteristic curve identified optimal cut-off values NP/LHb Mono, Kaplan-Meier revealed that higher levels both were associated significantly shorter novel model, NPM, integrating demonstrated superior predictive accuracy compared either marker alone. The NPM model further validated through a nomogram, achieving performance 1-, 3-, 5-year These findings highlight potential combining inflammatory nutritional effective risk stratification offers simple, cost-effective tool may facilitate personalized management, though prospective warranted.
Language: Английский
Citations
1
Journal of Cancer, Journal Year: 2024, Volume and Issue: 15(11), P. 3580 - 3595
Published: Jan. 1, 2024
Copper, an indispensable trace element for the human body, serves not only as a crucial auxiliary factor in redox reactions within organism but also significant constituent of numerous key metabolic enzymes.The COMMD family plays vital role regulating copper at both cellular and systemic levels, particularly realm tumor research, area notably deficient gastric cancer investigations.With advancement precision medical techniques, individualized precise screening treatment have become paramount considerations contemporary landscape therapy.In light this, we meticulously scrutinized existing transcriptomic datasets cancer, validating expression levels prognostic value genes.Simultaneously, employing ssGSEA algorithm, devised COMMDs score.Enrichment analysis, gene mutations, clinical features were incorporated into assessment this score.Furthermore, contextualized score framework immune microenvironment, evaluating relationship between factors well cells.The results suggest correlation various immune-related features.Based on foundation, multiple machine learning approaches indicated Logistic Regression, with remarkable ROC 0.972, optimal diagnostic model.To accentuate translational family, selected COMMD10 differential further validation.Functional experiments revealed decline proliferative migratory capabilities cells upon silencing COMMD10.Additionally, through pathway intervention, unveiled PI3K-AKT potential mechanism which influences activity.In summary, our study affirms prospective markers diagnosis future.
Language: Английский
Citations
4
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: Jan. 24, 2025
Background The convergence of macrophage-targeted strategies with immune checkpoint blockade therapies defines a pivotal avenue in contemporary tumor therapy. Identifying robust genetic regulators this context is imperative. Methods This study elucidates IFI30's role enhancing Major Histocompatibility Complex II (MHC-II) restriction antigen processing. Despite its recognition cancer immunotherapy, IFI30 remains nascent focus. Our approach involves multi-omics analysis immunological profile the macrophage-mediated Tumor Microenvironment (TME), spanning various cancers and bolstered by rigorous co-culture laboratory work. Results predominantly localizes monocyte/macrophage populations, correlating strongly cell infiltration. Substantiated single-cell analysis, exhibits significant functional enrichment immune-related pathways. Co-expression genes, including MHC elements checkpoints, further validates relevance. Conclusion positions as promising immunotherapeutic target. Pan-cancer analyses glioblastoma multiforme (GBM) investigations collectively underscore potential TME modulator, particularly interaction M2-macrophages. emerges prospective intervention point landscape.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: Feb. 26, 2025
Lung cancer remains a leading cause of cancer-related deaths worldwide, necessitating innovative treatments. Tumor-associated macrophages (TAMs) are primary immunosuppressive effectors that foster tumor proliferation, angiogenesis, metastasis, and resistance to therapy. They broadly categorized into proinflammatory M1 tumor-promoting M2 phenotypes, with elevated infiltration correlating poor prognosis. Strategies aimed at inhibiting TAM recruitment, depleting TAMs, or reprogramming therefore highly promising. Key signaling pathways, such as CSF-1/CSF-1R, IL-4/IL-13-STAT6, TLRs, CD47-SIRPα, regulate polarization. Additionally, macrophage-based drug delivery systems permit targeted agent transport hypoxic regions, enhancing Preclinical studies combining TAM-targeted therapies chemotherapy immune checkpoint inhibitors have yielded improved responses prolonged survival. Several clinical trials also reported benefits in previously unresponsive patients. Future work should clarify the roles macrophage-derived exosomes, cytokines, additional mediators shaping microenvironment. These insights will inform design next-generation carriers optimize combination immunotherapies within precision medicine frameworks. Elucidating phenotypes their regulatory molecules central developing novel strategies curb progression ultimately improve outcomes lung cancer. Importantly, immunomodulation may offer expanded treatment avenues.
Language: Английский
Citations
0
Journal of Cancer Research and Clinical Oncology, Journal Year: 2025, Volume and Issue: 151(4)
Published: April 10, 2025
This study aimed to evaluate the safety and efficacy of intestinal stent placement as a bridge surgery in patients with left colon cancer complicated by obstruction (LCCO). A retrospective cohort analysis was conducted on 111 diagnosed LCCO at The Second Affiliated Hospital Chongqing Medical University between January 2015 August 2019. Patients were divided into two groups: group (SG, n = 41) emergency (EG, 70). Primary endpoints included 3-year progression-free survival (PFS), local recurrence, distant metastasis rates. Secondary encompassed overall (OS), intraoperative parameters (lymph node dissection, blood loss, operative time), enterostomy rate, postoperative complications, hospital stay duration. No significant differences observed SG EG PFS (59% vs. 41%, P 0.091), OS (61% 44%, 0.051), or rates (19.5% 20%, 0.95). However, demonstrated superior short-term outcomes, including reduced loss (60 mL 78 mL, 0.02), shorter (10.2 16.1 days, < 0.001), lower rate (0% 100%, fewer complications (14.6% 24.3%, 0.012). Stenting can relieve symptoms time. Compared open surgery, it has better results does not affect long-term curative effect tumor.
Language: Английский
Citations
0
BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)
Published: May 1, 2025
Head and neck squamous cell carcinoma (HNSCC) is a lethal malignancy with high recurrence distant metastasis rate, posing significant challenges to patient prognosis. Recent studies suggest that tumor-associated neutrophils (TANs) can modulate immune infiltration influence tumor initiation progression. However, the potential clinical significance of TANs in HNSCC remains insufficiently explored. TANs-specific marker genes were identified via single-cell sequencing data from HNSCC. Based on The Cancer Genome Atlas (TCGA), prognostic risk model was constructed using genes, validated Gene Expression Omnibus (GEO) database. associations between signature characteristics, functional pathways, infiltration, checkpoint expression, responses immunotherapy chemotherapy, then investigated. Cell counting kit-8(CCK-8), Transwell, wound healing assays conducted assess role molecules. characteristic patients. On basis these neutrophils-associated (NRS) developed across internal external cross-platform cohorts through comprehensive procedures. NRS demonstrated robust reliable performance predicting overall survival. Additionally, patients low showed enhanced active lipid metabolism, increased sensitivity immunotherapy. In contrast, exhibited poor outcomes, advanced stages, Furthermore, we TANs-associated biomarker, OLR1, OLR1 promotes proliferation, invasion, migration CCK-8, Transwell assays. This study has promising TANs-based tool may aid personalized treatment management for
Language: Английский
Citations
0
SLAS TECHNOLOGY, Journal Year: 2024, Volume and Issue: 29(5), P. 100183 - 100183
Published: Aug. 31, 2024
Breast cancer (BC), a prevalent and severe malignancy, detrimentally affects women globally. Its prognostic implications are profoundly influenced by gene expression patterns. This study retrieved 509 BCE-associated oncogenes 1,012 neurotransmitter receptor-related genes from the GSEA KEGG databases, intersecting to identify 98 relevant genes. Clinical transcriptomic data related BC were downloaded TCGA, differential identified based on an FDR value <0.05 & |log2FC| ≥ 0.585. Univariate analysis of these revealed that high NSF low HRAS, KIF17, RPS6KA1 closely associated with survival prognosis. A model constructed for four demonstrated significant relevance BC-TCGA patients (P < 0.001). Subsequently, immunofunctional oncogene-neurotransmitter cluster involvement immune cells such as T CD8, CD4 memory resting, Macrophages M2. Further indicated functions primarily concentrated in APC_co_inhibition, APC_co_stimulation, CCR, Check-point, among others. Lastly, nomogram was established, ROC curve is vital indicator assessing prognosis, 1-year, 3-year, 5-year rates 0.981, 0.897, 0.802, respectively. demonstrates calibration, clinical utility, predictive capability, promising offer effective preliminary tool diagnostics.
Language: Английский
Citations
3
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: April 1, 2025
The dismal efficacy of immunotherapy for Pancreatic cancer (PC) can be predominantly ascribed to its distinctive cold-tumor properties. by-products purine metabolic reprogramming are extensively engaged in tumor immune modulation, influencing the functions and recruitment cells molding an microenvironment that is propitious growth. We harnessed single-cell transcriptomics spatial concurrently analyze metabolism (PM) features PC microenvironment. quantitatively appraised PM traits diverse cell subsets via scoring algorithms such as AUCell Ucell. Moreover, development cell-cell interaction analysis elucidated alterations TME induced by dysregulation. Additionally, we defined disorder characteristics patients utilized this assess phenotypes prognoses patient population. Also, identified crucial intermediate genes impact establishment immunosuppressive environment within PC, validated them through sectioning co-culture experiments. Multi - dimensional transcriptome data unique heterogeneity microenvironment, which manifested fibroblasts demonstrating higher scores TME. Cellchat revealed malignant with elevated expression were concomitantly associated frequent interactions CAFs well high ligand-receptor pairs transcription factors. Spatial further corroborated finding. Furthermore, newly constructed criteria indicated levels a lack response Finally, study singular role NT5E immunosuppression resulting from PC. CCK8 invasion experiments following model demonstrated intervention targeting could reverse augmented malignancy co-cultured CAFs. potentially key target reversing "stiff-cancer" This demonstrates disorders impinge upon exacerbate engendered progression fibrosis. Therapeutic strategies or may offer ray hope advanced PDAC.
Language: Английский
Citations
0
Thoracic Cancer, Journal Year: 2025, Volume and Issue: 16(7)
Published: April 1, 2025
ABSTRACT Background Esophageal squamous cell carcinoma (ESCC) is a prevalent and deadly cancer, making it essential to understand the molecular mechanisms influencing its development prognosis. The role of interferon‐gamma‐inducible protein 30 (IFI30) in antigen processing well‐established, but impact on progression ESCC remains unclear. This study aimed investigate biological function potential IFI30 progression. Methods Public databases, proteomics, immunohistochemistry (IHC) were employed analyze expression. Cell proliferation, migration, invasion evaluated using MTS, colony formation, wound healing, transwell assays. Nude mouse xenograft models established assess effects knockdown vivo. Quantitative proteomics was utilized identify differentially expressed proteins (DEPs) pathways altered by knockdown. apoptosis senescence flow cytometry, SA‐β‐gal staining, reactive oxygen species (ROS) analysis. Results highly correlated with advanced stage poor inhibited vitro suppressed tumor growth DEPs mainly enriched related apoptosis, mitophagy, cellular senescence, lysosome. Furthermore, cells upregulated HRAS expression, increased ROS production, activated JNK signaling pathway, elevated expression P16 P21, thereby promoting senescence. Conclusions suggests that may regulate P21/P16 pathways, exerting pro‐tumorigenic ESCC. could serve as novel target for treatment.
Language: Английский
Citations
0