The inflammatory response to Escherichia coli lipopolysaccharide is mitigated by in ovo delivery of carvacrol in broiler chicks

Poultry Science, Journal Year: 2025, Volume and Issue: 104(3), P. 104881 - 104881

Published: Feb. 3, 2025

The broiler chickens' immune system fully develops a few weeks after hatching, leaving them susceptible to disease during the early post-hatching phase. This study investigated effects of in ovo delivery carvacrol on expression immune-related genes following an Escherichia coli lipopolysaccharide (LPS) challenge at d7 post-hatch. experiment was designed as 2 × factorial arrangement with two treatments (saline or carvacrol) and LPS challenged groups (yes no): (1) saline + no (control), (2) LPS, (3) challenge, (4) LPS. Hatchability performance parameters were determined before, organ weights challenge. Immune-related gene assessed jejunum spleen post-challenge. At d7, carvacrol-treated group had lower FI (Δ11 g, P = 0.02) compared group. In jejunum, significant interaction (P 0.003) found between treatment for IFN-γ expression, which highest groups. Carvacrol resulted IL-8 0.03) IκB 0.04) regardless spleen, showed that downregulated pro-inflammatory IL-1β, observed For NF-κB TLR4, did not affect saline-treated chickens, but lowered these chickens 0.03 0.02, respectively). differ higher than non-challenged These findings indicate anti-inflammatory effect by inhibiting LPS-induced downregulation mitigating IL-1β TNF-α expression. stimulated potentially modulating adaptive responses. conclusion, could mitigate release key inflammatory cytokines, while increasing IFN-γ.

Language: Английский

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Modern approaches to the use of ultrafine and nanoparticles as components of feed additives in poultry farming

World s Poultry Science Journal, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 40

Published: Feb. 18, 2025

Language: Английский

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Effects of Dietary Supplementation Using Phytobiotics with Different Functional Properties on Expression of Immunity Genes, Intestinal Histology, Growth, and Meat Productivity of Broiler Chickens

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 302 - 302

Published: March 25, 2025

The aim of this study was to investigate the expression immunity-related genes and morpho-histological features intestines, growth meat production broiler chickens when fed plant extracts with different functional components. Chickens in control group received a basic diet. feed experimental groups contained standardized various biologically active components such as extract common chicory inulin (INUL), St. John’s wort flavonoids (FLAV), maral root ecdysterone (ECDS), creeping thyme tannins (FLAV-TANN). results showed that application studied phytobiotics increased pro-inflammatory gene IL8, 2−ΔΔCT value ranging from 2.66 4.63. In case antimicrobial peptide AvBD9, ranged 1.66 8.16, depending on group. AvBD10 (2−ΔΔCT = 2.19) were decreased used 0.33). also found using diets accompanied by significant decrease height epithelial cells cecum mucous membrane INUL (61.3%, p ≤ 0.001), FLAV (60.0%, ECDS (48.2%, FLAV+TANN (67.6%, 0.001). It caused reduction depth crypts (38.4%, (32.3%, (50.9%, (53.4%, 0.001) groups. use all extracts, except thyme, changes size muscular elements intestinal walls; thus, thickness layer 1.5–2.0 times under influence chicory, 430.99 579.87 μm, respectively (p 1.9 151.59 μm consumption stock safety, leading an increase 4.1–7.5% live weights at end rearing period, thus contributing higher slaughter quality poultry; particular, breast muscles cockerels 16.9% 0.05), while 18.1% 0.05) 23.1% 0.01), respectively. Thus, chicken diet increases activates immunity, which indicates possibility replacing antibacterial drugs natural nutraceuticals.

Language: Английский

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Evaluation of the in vivo assessment of Lycopodium complanatum L. ssp. chamaecyparissus (A.Braun ex Mutel) Döll extracts on TNF-α and IL-6

South African Journal of Botany, Journal Year: 2025, Volume and Issue: 182, P. 266 - 271

Published: May 17, 2025

Language: Английский

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Porcine β-Defensin 114: Creating a Dichotomous Response to Inflammation

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1016 - 1016

Published: Jan. 13, 2024

The immunity-related functions of defensins seem to be dependent on environmental stimuli, the cell type, and concentration peptides. However, function mechanism porcine β-defensin 114 (pBD114) in regulating inflammatory response macrophages are unclear. Therefore, modulatory effects pBD114 were investigated by treating mouse monocyte macrophage line RAW264.7 with different concentrations or without lipopolysaccharide (LPS). RNA-seq analysis was performed investigate mechanisms underlying pBD114’s regulation responses macrophages. In addition, response-modulating also further verified a assay. results showed that 100 μg/mL significantly promoted secretion TNF-α IL-10 RAW264.7. LPS-induced increase TNFα cultures decreased 10 pBD114. These suggest can exhibit pro-inflammatory activities under normal physiological conditions pBD114, anti-inflammatory during an excessive gain insights into response. Among pBD114-promoting responses, up-regulated 1170 genes down-regulated 724 genes. KEGG enrichment differentially expressed (DEGs) enriched immune- signal-transduction-related signaling pathways. Protein-Protein Interaction (PPI) key driver (KDA) analyses revealed Bcl10 Bcl3 12 38 DEGs mainly “Cytokine–cytokine receptor interaction” pathway, PPI KDA Stat1 Csf2 qRT-PCR those RNA-seq. vivo tests confirmed pro- Although is rapid complex reaction noxious this study found plays essential role acting related immunity, signal transduction, molecules, interactions. conclusion, provides certain theoretical basis for research application defensins.

Language: Английский

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Co-infection of Fowl Cholera with bacterial and viral infection in poultry flocks

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 24, 2024

Abstract This study explores the co-infection dynamics of Pasteurella multocida ( P.multocida ) in poultry, focusing on Avian Leukosis Virus (ALV), Mycoplasma gallisepticum (MG), and Chicken Anemia (CAV). Co-infections pose significant challenges to poultry health, leading increased morbidity mortality rates. The combined impact exacerbates respiratory issues, weakens immune system, leads intricate clinical manifestations. Samples from flocks Telangana Haryana states India, suspected for fowl cholera, were collected during 2019–2021. confirms co-infections using conventional PCR techniques targeting specific gene regions. Clinical symptoms exhibited by infected birds are described each pathogen. epidemiology is discussed, importance understanding these effective control prevention strategies emphasized. Results reveal consistent over period, highlighting need further investigations into associations with parasites, bacteria, fungi, or viruses. underscores comprehensive biosecurity measures, vaccination programs, early detection managing ensuring sustainable production.

Language: Английский

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Effect of Dietary Benzoic Acid and Oregano Essential Oil as a Substitute for an Anti-Coccidial Agent on Growth Performance and Physiological and Immunological Responses in Broiler Chickens Challenged with Eimeria Species

Animals, Journal Year: 2024, Volume and Issue: 14(20), P. 3008 - 3008

Published: Oct. 17, 2024

To overcome the antimicrobial residues in food, benzoic acid (BA) and oregano essential oil (OEO) are used broiler chicken industry. Independently, both exerted anticoccidial actions improved growth performance chickens. Their effect may be multiplied when they combination. This present study was carried out to evaluate efficacy of dietary BA OEO alone or combination as a substitute for commercial coccidiostatic drug on physiological immunological responses chickens challenged with

Language: Английский

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Porcine β-defensin 114: Creating a Dichotomous Response to Inflammation

Published: Dec. 8, 2023

This study explored the effects of pBD114 (porcine β-defensin 114) on inflammatory response. The results in RAW264.7 cells showed that 100 μg/mL significantly increased concentrations TNFα and IL-10, 10 decreased concen-tration LPS-induced cell cultures at ng/mL. These suggest can exhibit pro-inflammatory or anti-inflammatory activities under certain conditions. RNA-seq analysis was performed to gain further insight into in-flammatory Among promoting response, up-regulated 1170 genes down-regulated 724 genes. KEGG enrichment found DEGs were enriched immune- signal-transduction-related signaling pathways. PPI KDA analyses revealed Bcl10 Bcl3 key In addition, 12 38 an-ti-inflammatory mainly en-riched "Cytokine-cytokine receptor interaction" pathway, Stat1 Csf2 qRT-PCR verified those RNA-seq. vivo mice tests also confirmed pro- activity pBD114. Although response is a rapid complex physiological reaction noxious stimuli, this plays an essential role by acting related immunity, signal transduction, molecules, interactions.

Language: Английский

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Saccharomyces Boulardii and Saccharomyces Cerevisiae Improve Immunity in Broilers Vaccinated Against Pasteurella Multocida and Salmonella Gallinarum

Brazilian Journal of Poultry Science, Journal Year: 2024, Volume and Issue: 26(4)

Published: Jan. 1, 2024

ABSTRACT This study evaluated the impact of dietary Saccharomyces boulardii and cerevisiae on immunity birds vaccinated against Pasteurella multocida Salmonella gallinarum. A total 105 male Cobb 500 broilers were divided into four groups: T1 (vaccinated, no supplement, n = 30), T2 S. T3 T4 (non-vaccinated, 15). Chickens received a corn-soy diet with 1x107 CFU/g or for 42 days. Immune response was assessed by indirect ELISA leukocyte counts. At 21 days, both supplemented groups showed significantly higher IgY levels than control (p < 0.05). supplementation increased lymphocytes 0.003) decreased heterophils (p=0.004), while had significant effect. The heterophil/lymphocyte ratio respectively 23.4% 32.8% in at changes These results indicate that can boost overall health broilers.

Language: Английский

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Unveiling the Antimicrobial Potential of Terminalia chebula Fruits in Poultry Feed: A Natural Alternative for Enhanced Food Safety and Animal Health

UTTAR PRADESH JOURNAL OF ZOOLOGY, Journal Year: 2023, Volume and Issue: 44(24), P. 218 - 224

Published: Dec. 26, 2023

Background: Bacterial contamination of poultry feed poses a significant risk to both and consumers. The use antibiotics combat microbial in has raised health concerns, emphasizing the need for alternative methods ensure food safety. Aim: This study aimed investigate antimicrobial potential Terminalia chebula fruits (T. chebula) as additive production. sought assess effectiveness T. eradicating poultry-associated microbes explore its additive. Methodology: involved collection extraction fruits, qualitative phytochemical studies, isolation bacterial pathogens from samples. activity was determined using agar well diffusion method. GCMS analysis employed identify various secondary metabolites present chebula. Results: studies showed that methanol chloroform extracts exhibited efficacy against Gram-positive Gram-negative bacteria. revealed presence phytochemicals fatty acid compounds contributed chebula. also found superior antibacterial compared extract. Implications: highlights sustainable feed. offers innovative solutions safety, improve animal health, reduce reliance on

Language: Английский

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