Cytokines as potential biomarkers for the diagnosis of Mycobacterium bovis infection in Mediterranean buffaloes (Bubalus bubalis) DOI Creative Commons
Giulia Franzoni, Federica Signorelli, Piera Mazzone

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 24, 2024

Mycobacterium bovis ( M. ) is the primary agent of bovine tuberculosis (TB) in Mediterranean buffalo, which has a negative economic impact on buffalo herds. Improving TB diagnostic performance this species represents key step to eradicate efficiently disease. We have recently shown utility IFN-γ assay diagnosis infection buffaloes Bubalus bubalis ), but other cytokines might be useful immunological biomarkers infection. therefore investigated immune as species. Thirty-six Italian were used study: healthy animals N = 11), infected (IFN-γ test positive, no post-mortem lesions, detection; 14), and affected visible lesions; 11). Heparin blood samples stimulated with purified protein derivative (PPD-B), alongside controls, 18–24 h later plasma collected. Levels 14 measured: IFN-γ, IL-17, IL-4, IL-10, TNF, IL-1α, IL-1β, IL-6, IP-10, MIP-1α, MIP-1β, MCP-1, IL-36Ra, VEGF-A. observed that both released higher levels response PPD-B compared subjects. also IL-1β IP-10 subjects, whereas only tendencies detected animals. Affected MIP-1α subjects group values specific TNF was observed. Finally, canonical discriminant analysis (CDA) generate predictive cytokine profiles by groups. Our data suggest MIP-1β could Buffalo can improve specie.

Language: Английский

Genomic Tools for Medicinal Properties of Goat Milk for Cosmetic and Health Benefits: A Narrative Review DOI Open Access
Keabetswe Tebogo Ncube, Mamokoma Catherine Modiba, Takalani Judas Mpofu

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 893 - 893

Published: Jan. 22, 2025

Goat milk has gained recognition for its medicinal, cosmetic, and health benefits, particularly potential to improve human skin conditions. Its therapeutic properties are attributed bioactive compounds influenced by genes such as lactoferrin (LTF), lysozyme (LYZ), β-casein (CSN2), known their antimicrobial, immunomodulatory, anti-inflammatory effects. Genetic factors hypothesized shape goat milk’s composition effectiveness in managing dermatological conditions like eczema psoriasis. Understanding these genetic determinants is critical optimizing the use of applications. This review aims explore application genomic tools elucidate medicinal implications care. By identifying specific molecular mechanisms underpinning effects, studies have provided insights into constituents milk, peptides, proteins, lipids, which contribute efficacy. Candidate genes, including growth hormone receptor (GHR), butyrophilin (BTN1A1), lactoglobulin (LGB), been identified enhancing quality functionality. Future research should integrate data with functional further investigate antioxidant activities. Such could advance targeted breeding strategies innovative formulations inflammatory promoting health.

Language: Английский

Citations

1
Milk-derived extracellular vesicles and gut health DOI Creative Commons
Muttiah Barathan, Jia Xian Law

npj Science of Food, Journal Year: 2025, Volume and Issue: 9(1)

Published: Jan. 30, 2025

Language: Английский

Citations

0
Oral delivery of extracellular vesicles from goat milk alters the microbiota, enhances the immune response, and strengthens the barrier function in the intestine of mice DOI
Ziwei Wang, Xinlong Zhang, Rui Chen

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106052 - 106052

Published: Feb. 1, 2025

Language: Английский

Citations

0
Goat Milk: A Versatile Dairy Alternative with Unique Health Benefits and Functional Properties DOI
Arun Kumar Verma, Tarun Pal Singh,

V. Rajkumar

et al.

Food Reviews International, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 36

Published: March 19, 2025

Globally goat milk ranks as the third most produced milk, following cow and buffalo milk. It is distinguished by a unique nutritional profile enriched with bioactive components that offer numerous therapeutic benefits. Its distinct protein, such low αs1-casein well higher β-casein, fat composition, like smaller globules concentrations of medium-chain triglycerides, taurine, polyamines, oligosaccharides, peptides, essential minerals, vitamins, contribute to enhanced digestibility nutrient absorption. Research reports indicate aforesaid features collectively impart hypoallergenic, antioxidant, antipathogenic, immune-modulating, anti-inflammatory, anti-proliferative effects, supporting brain health, cardiovascular function, gut health. Because these properties, are being applied for different physiological health-boosting potentials. Also, constituents currently under investigation their potential have facilitated development range specialty products. This review explores milk's exceptional functionality, medicinal value, highlighting its expanding role in consumer health wellness.

Language: Английский

Citations

0
Goat Milk-Derived Extracellular Vesicles Alleviate Colitis Potentially Through Improved Gut Microbiota in Mice DOI Creative Commons
Xinru Wang, Yi Liu, Chang Hong

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(9), P. 1514 - 1514

Published: April 26, 2025

Ulcerative colitis (UC) is characterized clinically by intestinal inflammation and gut microbiota dysbiosis. The consumption of biologics, although effective in control, may lead to adverse effects inconvenient for at-home administration. Goat milk-derived extracellular vesicles (GMEVs) have been proposed as a supplement prevent inflammation. However, their therapeutic potential remains elusive. This study aimed explore the preventive effect GMEVs on its underlying mechanisms through microbiota-immune axis using dextran sodium sulfate (DSS)-induced mouse model. We found that pre-treatment 20 mg/kg/d effectively prevented body weight loss, colon shortening, depletion colonic goblet cells, disappearance crypts, while enhancing mucosal barrier. Consistent with these phenotypes, GMEV increased levels IL-22 IL-10 decreased IL-1β, TNF-α, IL-6, iNOS. themselves had no normal mice. Paralleling alleviation inflammation, also restored reduction unclassified Muribaculaceae, Dubosiella, Lactobacillus suppressed expansion Alistipes Proteobacteria following DSS treatment. Additionally, intake significantly downregulated expression proteins NF-κB signaling pathway induced DSS. In summary, could regulating barrier, microbiota, organ damage, immune microenvironment. demonstrated application prospects UC prevention.

Language: Английский

Citations

0
Orally Delivered Milk-Derived Nanovesicles Loaded with Connexin 43 Peptides for Targeted Cardiac Ischemia-Reperfusion Therapy DOI Open Access
Spencer R. Marsh, Md Ruhul Amin, Stefano Toldo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Abstract Extracellular vesicles have emerged as promising nanocarriers for targeted drug delivery, but their therapeutic potential is limited by challenges related to administration route, loading, delivery and production at scale. Here, we report an innovative approach of peptides injured tissues using milk-derived small extracellular (mEVs) abundant, safe, orally administrable nanoplatform. We demonstrate that a sub-population mEVs naturally contain Connexin 43 (Cx43) its Carboxyl-Terminal (CT) polypeptides, which been shown play crucial roles in wound healing tissue repair. Leveraging this intrinsic property, developed esterification method efficiently uniformly load with enhanced levels exogenous Cx43 CT peptide (αCT11 - RPRPDDLEI), assessed flow cytometry-based vesicle quantification mass spectrometry. These engineered exhibited remarkable injury targeting capabilities, > 30-fold increases uptake cells compared non-wounded vitro preferential accumulation wounded vivo. Notably, αCT11-loaded administered after myocardial infarction reduced infarct size >60% preserved heart function mouse model ischemia-reperfusion injury. This study represents significant advance nanomedicine, demonstrating the utilization occurring oral system peptides, achieving unprecedented efficiency efficacy treatment

Language: Английский

Citations

0
Harnessing the power of goat milk-derived extracellular vesicles for medical breakthroughs: A review DOI
Yiming Meng,

Jing Sun,

Guirong Zhang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 262, P. 130044 - 130044

Published: Feb. 8, 2024

Language: Английский

Citations

3
Goat milk extracellular vesicles: Separation comparison of natural carriers for theragnostic application DOI Creative Commons

Jessie Santoro,

Silvia Nuzzo, Monica Franzese

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(6), P. e27621 - e27621

Published: March 1, 2024

Goat milk is a complex biological fluid, which in addition to having high nutritional value, it an interesting source of extracellular vesicles (EVs). Despite the countless potential applications that they offer many fields, not easy compare different proposed systems, and this major limitation for real translatability these natural nanoplatforms theragnostic purposes. Thus, useful further investigate reproducible methods separate goat EVs. The choice but also preprocessing has immense impact on separation, quality, yield Here, we tested four protocols EVs from unpasteurised milk: two based differential ultracentrifugation (DUC) size-exclusion chromatography (SEC). Moreover, assessed approaches pre-treatment (acidification precipitation) facilitate protein discharge. To best our knowledge, similar comparison all performed raw never been published before. Therefore, enriched EV samples were successfully obtained using both DUC SEC. Taken together, results may be helpful obtain carriers future personalised medicine.

Language: Английский

Citations

2
Milk-Derived Extracellular Vesicles: A Novel Perspective on Comparative Therapeutics and Targeted Nanocarrier Application DOI Creative Commons
Muttiah Barathan, Sook Luan Ng, Yogeswaran Lokanathan

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(11), P. 1282 - 1282

Published: Nov. 15, 2024

Milk-derived extracellular vesicles (mEVs) are emerging as promising therapeutic candidates due to their unique properties and versatile functions. These play a crucial role in immunomodulation by influencing macrophage differentiation cytokine production, potentially aiding the treatment of conditions such bone loss, fibrosis, cancer. mEVs also have capacity modulate gut microbiota composition, which may alleviate symptoms inflammatory bowel diseases promote intestinal barrier integrity. Their potential drug delivery vehicles is significant, enhancing stability, solubility, bioavailability anticancer agents while supporting wound healing reducing inflammation. Additionally, bovine exhibit anti-aging protect skin cells from UV damage. As vaccine platforms, offer advantages including biocompatibility, antigen protection, ability elicit robust immune responses through targeted specific cells. Despite these applications, challenges persist, complex roles cancer, effective loading, regulatory hurdles, need for standardized production methods. Achieving high targeting specificity understanding long-term effects mEV-based therapies essential clinical translation. Ongoing research aims optimize mEV methods, enhance capabilities, conduct rigorous preclinical studies. By addressing challenges, hold revolutionize development delivery, ultimately improving outcomes across various medical fields.

Language: Английский

Citations

1
Milk-Derived Extracellular Vesicles Carrying ssc-let-7c Alleviate Early Intestinal Inflammation and Regulate Macrophage Polarization via Targeting the PTEN-Mediated PI3K/Akt Pathway DOI

Dongdong Lu,

Xiangyu Zhang, Hao Ye

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(40), P. 22092 - 22104

Published: Aug. 27, 2024

Milk-derived extracellular vesicles (mEVs) are beneficial to the health of infants. However, effect mEVs on early intestinal inflammation is not well established. Herein, weaned colitic mice were used explore potential effects and underlying mechanisms porcine (pmEVs) during life. We found that pmEVs administration attenuated life promoted colonic barrier integrity in mice. The anti-inflammatory was achieved by shifting a proinflammatory macrophage (M1) toward an (M2). Moreover, can be absorbed macrophages reduce polarization (stimulated LPS) vitro. Noteworthily, ssc-let-7c highly expressed regulate targeting tensin homologue deleted chromosome ten (PTEN), thereby activating PI3K/Akt pathway. Collectively, our findings revealed crucial role immunity its mechanism.

Language: Английский

Citations

0