Poultry Science, Journal Year: 2024, Volume and Issue: unknown, P. 104436 - 104436
Published: Oct. 1, 2024
Language: Английский
Molecular Biomedicine, Journal Year: 2024, Volume and Issue: 5(1)
Published: April 29, 2024
Abstract Tuberculosis (TB) is an infectious disease that significantly threatens human health. However, the differential diagnosis of latent tuberculosis infection (LTBI) and active (ATB) remains a challenge for clinicians in early detection preventive intervention. In this study, we developed novel biomarker named HP16118P, utilizing 16 helper T lymphocyte (HTL) epitopes, 11 cytotoxic (CTL) 8 B cell epitopes identified from 15 antigens associated with LTBI-RD using IEDB database. We analyzed physicochemical properties, spatial structure, immunological characteristics HP16118P various tools, which indicated it hydrophilic relatively stable alkaline protein. Furthermore, exhibited good antigenicity immunogenicity, while being non-toxic non-allergenic, potential to induce immune responses. observed can stimulate production high levels IFN-γ + lymphocytes individuals ATB, LTBI, health controls. IL-5 induced by demonstrated distinguishing LTBI ATB patients ( p =0.0372, AUC=0.8214, 95% CI [0.5843 1.000]) sensitivity 100% specificity 71.43%. incorporated GM-CSF, IL-23, IL-5, MCP-3 into machine learning algorithms construct model. It was found Quadratic discriminant analysis model best diagnostic performance discriminating between 1.00, 0.86, accuracy 0.93. summary, has strong induction MCP-3, suggesting their ATB.
Language: Английский
Citations
10
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: April 16, 2024
Haemophilus parainfluenzae is a Gram-negative opportunist pathogen within the mucus of nose and mouth without significant symptoms has an ability to cause various infections ranging from ear, eye, sinus pneumonia. A concerning development increasing resistance H. beta-lactam antibiotics, with potential dental or abscesses. The principal objective this investigation utilize bioinformatics immuno-informatic methodologies in candidate multi-epitope Vaccine. focuses on identifying epitopes for both B cells (B lymphocytes) T (helper lymphocytes cytotoxic based high non-toxic non-allergenic characteristics. selection process involves human leukocyte antigen alleles demonstrating strong associations recognized antigenic overlapping epitopes. Notably, chosen aim provide coverage 90% global population. Multi-epitope constructs were designed by using suitable linker sequences. To enhance immunological potential, adjuvant sequence was incorporated EAAAK linker. final vaccine construct, comprising 344 amino acids, achieved after addition adjuvants linkers. This Vaccine demonstrates notable antigenicity possesses favorable physiochemical three-dimensional conformation underwent modeling refinement, validated through in-silico methods. Additionally, protein-protein molecular docking analysis conducted predict effective binding poses between Toll-like receptor 4 protein. Molecular Dynamics (MD) docked TLR4-vaccine complex demonstrated consistent stability over simulation period, primarily attributed electrostatic energy. displayed minimal deformation enhanced rigidity motion residues during dynamic simulation. Furthermore, codon translational optimization computational cloning performed ensure reliability proper expression multi-Epitope It crucial emphasize that despite these validations, experimental research laboratory imperative demonstrate immunogenicity protective efficacy developed vaccine. would involve practical assessments ascertain real-world effectiveness
Language: Английский
Citations
8
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 282, P. 136827 - 136827
Published: Oct. 30, 2024
Language: Английский
Citations
6
Synthetic and Systems Biotechnology, Journal Year: 2024, Volume and Issue: 9(4), P. 667 - 683
Published: May 18, 2024
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Citations
5
Heliyon, Journal Year: 2024, Volume and Issue: 10(11), P. e31744 - e31744
Published: May 31, 2024
BackgroundMultiple sclerosis (MS) is a heterogeneous autoimmune disease, with rapidly evolving body of literature on disease-modifying therapy (DMT) that urgently needs to be synthesized and regularized.MethodsThe original material used for the analysis was obtained from Web Science Core Collection (WoSCC) in Citation Index Expanded Edition (SCI-E). The data accessed through VOSviewer, Citespace, R package "Bibliometrix", Scimago Graphica visualization. Among them, clustering algorithm based Largest Likelihood Ratio (LLR) burst citation key.ResultsAs November 6th, 2022, 4142 publications related emerging therapies (e-DMT) MS, 6521 traditional (t-DMT) 1793 cross-cutting (I-DMT) MS were included analysis, respectively. Publications DMT analyzed descriptively (for three subjects: country, institution, author) predictively two keywords references) separately according sections: e-DMT, t-DMT, I-DMT. Topics still have relevant reference output as 2022 include safety Coronavirus disease 2019 (COVID-19) mRNA vaccination, therapeutic inertia (TI), cladribine tablets, autologous hematopoietic stem cell transplantation (aHSCT), progressive multiple sclerosis, pediatric sclerosis.ConclusionThe future research focus combination trial or cross-trial various treatment methods improve development individualized plans patients. exact contents frontiers are but not limited ocrelizumab, fingolimod other monoclonal antibodies, fumaric acid ester, tablet, aHSCT, interventions randomized controlled trials (RCTs); impact COVID-19 vaccination patients; TI, patient adherence, medical management issues; continued exploration biomarkers more accurate classification existing clinical indication classification.
Language: Английский
Citations
4
Decoding Infection and Transmission, Journal Year: 2024, Volume and Issue: 2, P. 100026 - 100026
Published: Jan. 1, 2024
Language: Английский
Citations
4
Infectious Medicine, Journal Year: 2024, Volume and Issue: 3(4), P. 100148 - 100148
Published: Nov. 9, 2024
Tuberculosis (TB) remains a global public health challenge. The existing Bacillus Calmette-Guérin vaccine has limited efficacy in preventing adult pulmonary TB, necessitating the development of new vaccines with improved protective effects.
Language: Английский
Citations
4
Veterinary Medicine International, Journal Year: 2025, Volume and Issue: 2025(1)
Published: Jan. 1, 2025
African swine fever (ASF), caused by virus (ASFV), is a highly contagious disease with devastating effects on the global pig industry. This warrants development of effective control strategies, such as vaccines. However, previously developed inactivated vaccines have proven ineffective, while live‐attenuated carry inherent safety risks. The use mRNA eliminates these risks offering safe, cost‐effective, and efficient vaccine strategy against ASFV. In this study, reverse vaccinology approach was used to design multiepitope pan‐proteomic Various bioinformatics tools were employed predict epitopes for cytotoxic T lymphocytes, helper B lymphocytes. A 50S ribosomal L7/L12 protein adjuvant, 5′ cap, poly(A) tail, signal peptide, MHC‐I‐targeting domain incorporated into using appropriate linkers increase immunogenicity, stability, recognition efficiency. physicochemical properties final construct evaluated, docking analyses done Toll‐like receptors (TLRs) 3, 4, 7 evaluate binding affinity. molecular dynamics simulation then performed determine immune simulations evaluated host’s response. Based 100 ASFV proteomes, six that induce T‐cell responses, five four antibody production predicted. designed found be nonallergenic, antigenic, stable when bound TLR4 pocket TLR3 TLR7 indicate high translation Immune demonstrated successful induction responses generation antigen‐specific memory cells. conclusion, study introduces an potential ASF in vitro confirmation.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: Jan. 31, 2025
Background Uveitis, characterized by intraocular inflammation, poses significant clinical challenges, often leading to vision impairment or blindness. Herpes Simplex Virus type 1 (HSV-1) is a major cause of virus-induced uveitis. This study aims design novel multi-epitope vaccine targeting HSV-1 glycoproteins B, C, D, H, and L using an immuno-informatics approach, which are essential for viral entry pathogenesis. Methods The identified epitopes CD8+ T cells, CD4+ B cells within the target glycoproteins. These were systematically evaluated conservancy, immunogenicity, non-allergenicity, non-glycosylated regions, binding affinities. A construct was designed, incorporating these along with adjuvant, PADRE sequence, suitable linkers. In-silico immune simulations performed evaluate vaccine’s potential activate both innate adaptive responses. Molecular docking assessed interactions between Toll-like receptor (TLR-9). Results selected demonstrated high non-allergenicity. effectively activated cytokine production, immunoglobulin secretion, cell responses in in-silico simulations. showed strong TLR-9, suggesting enhanced antigen presentation capabilities. Conclusion comprehensive approach provides precision immunotherapy strategy uveitis leveraging computational modeling predictive analytics HSV-1. results indicate efficacy activating Future experimental validation studies necessary confirm safety this proposed managing preserving vision.
Language: Английский
Citations
0
Next research., Journal Year: 2025, Volume and Issue: unknown, P. 100365 - 100365
Published: April 1, 2025
Language: Английский
Citations
0