Exploring the causal relationship between inflammatory cytokines and C-reactive protein levels and atopic dermatitis: a two-way two-sample Mendelian randomization study DOI
Linfeng Li,

Yizhao Ma,

Zuohu Niu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract Objective: Atopic dermatitis (AD) is an allergic inflammatory skin disease. Changes in circulating proteins are reflected the entire process of AD progression, and its pathophysiology still unclear. This Mendelian randomization study was conducted to further evaluate role AD. Methods: investigated potential causal relationship between We used a two-sample (MR) method analyze data from large-scale genome-wide association explore 91 proteins, 41 factors, CRP The inverse variance weighted mainly exposure outcome based on effect indicator odds ratio (OR) 95% confidence interval (CI). In addition, MR-Egger, median, simple model, model MR-PRESSO multiple sensitivity analyses were applied strengthen final results. leave-one-out method, heterogeneity test, horizontal gene pleiotropy test verify stability reliability Results: Forward MR analysis showed that there significant correlation risk changes levels at different p-value thresholds, among which increased interleukin-18 receptor 1 found increase AD, all three groups (P IVW<0.05); C-X-C motif chemokine 9 Fms-related tyrosine kinase 3 ligand reduce P<5×10-8 p<5×10-7 thresholds; 11 be associated with reduced P<5×10-8 and P<5×10-6 thresholds (P IVW<0.05). Reverse decreased AXIN-1, natural killer cell 2B4, interleukin-1 subunit α, interleukin-33 IVW<0.05). Cystatin D factor sets, may IL18 IVW=0.036) MIG IVW=0.046). No observed analysis. After verification analysis, it such as ligand, interleukin 18 1, 9, tumor necrosis superfamily member 14 risk, consistency P value thresholds. Bidirectional complex bidirectional results stable, no instrumental variables had strong impact results, verified robustness There reverse level validation set. Conclusion: The indicate provides new approach for exploring biological mechanisms future proposes possible therapeutic targets. Further research needed confirm these understand specific provide reference studies

Language: Английский

Two-sample Mendelian randomization analysis of 91 circulating inflammatory protein levels and amyotrophic lateral sclerosis DOI Creative Commons

Chenxu Xiao,

Xiaochu Gu,

Yu Feng

et al.

Frontiers in Aging Neuroscience, Journal Year: 2024, Volume and Issue: 16

Published: March 27, 2024

Introduction Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease with poorly understood pathophysiology. Recent studies have highlighted systemic inflammation, especially the role of circulating inflammatory proteins, in ALS. Methods This study investigates potential causal link between these proteins and We employed two-sample Mendelian Randomization(MR) approach, analyzing data from large-scale genome-wide association to explore relationship 91 included various MR methods like Egger, weighted median, inverse-variance weighted, complemented by sensitivity analyses for robust results. Results Significant associations were observed levels including Adenosine Deaminase, Interleukin-17C, Oncostatin-M, Leukemia Inhibitory Factor Receptor, Osteoprotegerin, ALS risk. Consistencies noted across different P -value thresholds. Bidirectional suggested that risk might influence certain proteins. Discussion Our findings, via analysis, indicate sheds new light on pathophysiology suggests possible therapeutic targets. Further research required confirm results understand specific roles

Language: Английский

Citations

6
Causal relationship between gut microbiota and chronic renal failure: a two-sample Mendelian randomization study DOI Creative Commons

Xingzheng Liu,

Jinying Mo,

Xuerui Yang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 3, 2024

Background Observational studies and some experimental investigations have indicated that gut microbiota are closely associated with the incidence progression of chronic renal failure. However, causal relationship between failure remains unclear. The present study employs a two-sample Mendelian randomization approach to infer at genetic level. This research aims determine whether there is effect on risk failure, aiming provide new evidence support targeted therapy for treatment Methods Employing genome-wide association (GWAS) data from public MiBioGen IEU OpenGWAS platform, analysis was conducted. inferred using five different methods: Inverse Variance Weighted, MR-Egger, Weighted Median, Simple Mode, Mode. incorporated sensitivity analyses encompassed evaluations pleiotropy heterogeneity. Subsequently, results underwent stringent correction multiple testing, employing False Discovery Rate method enhance validity our findings. Results According method, seven bacterial genera show significant outcome variable Of these, Ruminococcus (gauvreauii group) (OR = 0.82, 95% CI 0.71–0.94, p 0.004) may act as protective factor against while Escherichia-Shigella 1.22, 1.08–1.38, 0.001), Lactococcus 1.1, 1.02–1.19, 0.013), Odoribacter 1.23, 1.03–1.49, 0.026), Enterorhabdus 1.14, 1.00–1.29, 0.047), Eubacterium (eligens 1.18, 1.02–1.37, 0.024), Howardella 1.09–1.28, &lt; 0.001) be factors after comparisons Rate, only associations remain significant, indicating other suggestive associations. Sensitivity did not reveal any or Conclusion Our suggests they serve potential targets future therapeutic interventions. exact mechanisms action yet clear, necessitating further elucidate their precise roles fully.

Language: Английский

Citations

5
Effect of konjac glucomannan on gut microbiota from hyperuricemia subjects in vitro: fermentation characteristics and inhibitory xanthine oxidase activity DOI Creative Commons
Jie Deng, Kai Zhou,

Caimin Feng

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Sept. 19, 2024

Background The disorder of uric acid metabolism is closely associated with gut microbiota and short-chain fatty acids (SCFAs) dysregulation, but the biological mechanism unclear, limiting development acid-lowering active polysaccharides. Konjac glucomannan (KGM) could attenuate metabolic disturbance modulate microbiota. However, relationship between still unknown. Methods In this study, fecal samples were provided by healthy volunteers hyperuricemia (HUA) patients. Fecal from was regarded as NOR group. Similarly, 10% HUA suspension named Then, supernatant inoculated into a growth basal medium containing glucose or KGM, designated NOR-GLU NOR-KGM groups, while HUA-GLU HUA-KGM groups. All cultured in an anaerobic bag system. After fermentation for 24 h, collected further analysis composition intestinal microbiota, SCFAs concentration XOD enzyme activity. Results results showed that KGM be utilized degraded subjects, it structure their to more resemble addition, superior modulated effect on increasing Megasphaera , Faecalibacterium Lachnoclostridium Lachnospiraceae Anaerostipes Ruminococcus levels decreasing Butyricicoccus Eisenbergiella Enterococcus levels. Furthermore, solution inhibitory xanthine oxidase (XOD) activity, which might due metabolites such SCFAs. Conclusion conclusion, subjects investigated based vitro . present study. It found metabolized At genus level, decrease relative abundances significantly increased addition KGM. SCFAs, responsible inhibition Thus, exhibited probiotic function expected promising candidate remodeling

Language: Английский

Citations

5
Association of methyl donor nutrients dietary intake and sleep disorders in the elderly revealed by the intestinal microbiome DOI
Qianqian Chen, Rui Fan,

Lixia Song

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(12), P. 6335 - 6346

Published: Jan. 1, 2024

Currently, sleep disorders (SD) in the elderly are gaining prominence globally and becoming a significant public health concern. Methyl donor nutrients (MDNs), such as vitamin B6, B12, folate, choline, have been reported to potential improve disorders. Moreover, MDNs help maintain gut flora homeostasis, closely associated with development of SD. Nevertheless, there has lack comprehensive human studies examining association between intake In our study, we comprehensively evaluated SD used 16S rRNA gene sequencing explore underlying mechanism. We found that group (n = 91) had lower methyl-donor nutritional quality index (MNQI) trend toward compared control 147). Based on intestinal microbiome, beta diversity was higher high (HQ) low (LQ) group, it group. This suggests may regulate by modulating abundance distribution microbiota. Subsequently, performed correlation analyses relative microbiota, intake, Pittsburgh Sleep Quality Index (PSQI), identifying five genera regulatory roles. The KEGG pathway analysis indicated energy metabolism one-carbon might be pathways through which modulate sleep. study offers dietary guidance strategies for managing provides insights targeted microbiota intervention.

Language: Английский

Citations

4
Causal Association between the Gut Microbiota and Chronic Kidney Disease: A Two-Sample Mendelian Randomization Study in a Chinese Population DOI
Wenjian Lin, Zixin Liang,

Junxuan Fang

et al.

Published: Jan. 1, 2025

Background: Differences in the gut microbiota and related metabolites across populations may influence their causal relationship with chronic kidney disease (CKD), which remains unclear Chinese population.

Language: Английский

Citations

0
Investigating the role of gut microbiota in diabetic nephropathy through plasma proteome mediated analysis DOI Creative Commons
Shaojie Fu, Fan Li, Jinyu Yu

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 14, 2025

Language: Английский

Citations

0
The Gut Microbiome as a Risk Factor for Future Chronic Kidney Disease DOI Creative Commons
Tapio Hellman,

Li-Fang Yeo,

Joonatan Palmu

et al.

Kidney International Reports, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Total alkaloids of Aconitum carmichaelii Debx alleviate cisplatin-induced acute renal injury by inhibiting inflammation and oxidative stress related to gut microbiota metabolism DOI

Xi Yang,

Yijing Xin,

Yanzhi Gu

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 135, P. 156128 - 156128

Published: Oct. 9, 2024

Language: Английский

Citations

3
Genome-wide pleiotropy analysis reveals shared architecture between renal traits and gastrointestinal tract diseases DOI Creative Commons
Si Li, Shuang Wu, Minghui Jiang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Abstract Background: Comorbidities between gastrointestinal tract (GIT) and renal diseases have been widely reported, but the shared genetic architecture of gut traits remains unclear. Objective: To investigate etiology causal relationships or involved in gut-renal axis. Methods: We explored global local correlations, pleiotropic effects at variants gene levels, associations pair-wise GIT diseases, as well potential target drugs by using latest large-scale genome-wide association study (GWAS) summary data five (BUN, eGFR, CKD, IgAN, KSD) four (PUD, GORD, IBD, IBS). Results: Renal were genetically correlated globally locally across eight 20 trait pairs (BUN-GORD, BUN-IBD, BUN-IBS, CKD-IBD, IgAN-IBD, KSD-PUD, KSD-GORD, KSD-IBS). Pleiotropic analysis identified 222 loci prioritized 169 genes for pairs, including 21 novel that not significant original GWASs, colocalized loci, 29 drug-targeting genes. Among rs3129861 HLA-DRA was potentially BUN-GORD (PP4 = 0.814). KIF5B is a eGFR-IBD CKD-IBD rs12572072 0.929) rs61844306 0.898), both which are eQTLs expressed cultured fibroblasts cells. CKD IBD also PVALEF with PP4 0.800 rs138610699. In addition, rs6873866 casual variant ERAP2 IgAN with PP4=0.800, rs6873866-C allele negatively associated expression multiple tissues. Furthermore, tissue cell-type specific enrichment found over-expressed kidney cortex, immune-related tissues cell types. Mendelian randomization revealed nominal observed on IBS, PUD GORD eGFR. Conclusion: These findings suggested highlighted analyses drug repurposing comorbidities

Language: Английский

Citations

0
Association between the aggregate index of systemic inflammation and CKD: evidence from NHANES 1999–2018 DOI Creative Commons

Dongli Huang,

Hang Wu

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: March 10, 2025

Purpose We aimed to investigate the potential association between aggregate index of systemic inflammation (AISI) and chronic kidney disease (CKD). Patients methods This study analyzed data from National Health Nutrition Examination Survey (NHANES) spanning 1999 2018. CKD was defined as either an estimated glomerular filtration rate (eGFR) less than 60 mL/min/1.73 m 2 or presence albuminuria, a urine albumin-to-creatinine ratio (ACR) 30 mg/g higher. Low eGFR is . Multivariate regression analysis, smoothed curve fitting, subgroup analyses were conducted relationship Inflammatory status CKD. The receiver operating characteristic (ROC) analysis used evaluate its ability identify low eGFR. AISI transformed using natural logarithm (Ln) for statistical analysis. Results Of 50,768 recruits, 49.86% male. prevalence 20.31% 8.57%, respectively. Ln-AISI positively associated with (OR = 1.24; 95% CI: 1.19, 1.28) 1.17; CI:1.11, 1.24). Smooth fitting revealed positive Subgroup interaction tests indicated that stratifications did not significantly alter Threshold effect this became more pronounced when exceeded 5.2 (AISI &gt; 181.27). ROC showed had better discrimination accuracy identifying compared other inflammatory indicators [lymphocyte count (LYM), immune-inflammation (SII), platelet-to-lymphocyte (PLR), product platelet neutrophil (PPN)]. Conclusion correlated CKD, potent greater 181.27. Compared indicators, effective in

Language: Английский

Citations

0