Investigation of catechin’s anti-inflammatory activity: A bioinformatics and molecular docking study DOI Creative Commons
Nisa Yulianti Suprahman,

Bulan Rosita Sari,

Riri Fauziyya

et al.

Acta Biochimica Indonesiana, Journal Year: 2024, Volume and Issue: 7(2), P. 199 - 199

Published: Dec. 28, 2024

Background: Inflammation plays a key role in the progression of many chronic diseases. As country with rich biodiversity, Indonesia offers numerous phytochemicals potential for drug development, including catechin, natural compound anti-inflammatory properties. Objective: This study aimed to identify targets catechin and evaluate its inhibitory potency through molecular docking simulations. Methods: Data acquisition refinement were conducted using NCBI, STRING, STITCH databases, intersections identified Venn diagrams. Molecular was performed AutoDockTools 1.5.6, interactions visualized BIOVIA Discovery Studio. Results: Bioinformatics analysis predicted that inhibits three pro-inflammatory proteins: COX-2, HSP90, IL-2. Catechin’s indicated by negative binding energies amino acid residues critical target protein activity. Among targets, IL-2 exhibited lowest energy (-5.12 kcal/mol), suggesting it as primary target. However, catechin’s affinity lower than native ligand (-11.78 kcal/mol). Conclusion: is be Structural modifications are recommended enhance therapeutic potential.

Language: Английский

Increased intracellular stress responses and decreased KLF2 in adult patients with atopic dermatitis DOI Creative Commons
Shuji Sugiura, Haruka Yoshida, Hisashi Sugiura

et al.

Cell Stress and Chaperones, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Atopic dermatitis (AD) is prone to exacerbations in response various triggering factors and flare-ups after remission. We searched for molecules associated with relapse/exacerbation of AD among altered gene expression the skin patients AD. Microarray analyses were performed on lesional non-lesional adolescent or adult recalcitrant healthy controls. Five chaperones involved intracellular stress responses, namely heat shock protein family A (Hsp70) member 9 (HSPA9), 90 beta 1 (HSP90B1), calnexin (CANX), malectin (MLEC; endoplasmic reticulum-associated degradation), D (Hsp60) (HSPD1), consistently upregulated uninvolved Damage-associated molecular patterns skin. KLF transcription factor 2 (KLF2) was decreased exhibited a decreasing trend CD4(+)/CD8(+) double-positive cells (1.4% T cells) detected lesions declined KLF2 levels. WNT inhibitory (WIF1) downregulated Prolactin-induced only found increased responses Multifactorial genetic diseases, such as asthma, inflammatory bowel disease, type diabetes, rheumatoid arthritis, are stress. Intracellular abnormalities may also be responsible Further research incorporate enhanced into mechanism underlying

Language: Английский

Citations

1
The differential roles of heat shock protein 90 isoforms in skin inflammation: Anti-inflammatory potential of TRAP1 inhibition DOI
Hakim Ben Abdallah, Lars Iversen, Claus Johansen

et al.

Journal of Investigative Dermatology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
A tri-compound formula comprising ginsenoside Rg1, tetrandrine and icariin alleviates atopic dermatitis symptoms in a mouse model DOI
Ying Wu,

Xiaoqi Wang,

Jia‐Ying Wu

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156737 - 156737

Published: April 1, 2025

Language: Английский

Citations

0
HSP90 Complex From OLP Lesion Induces T‐Cell Polarization via Activation of Dendritic Cells DOI
Lei Pan, Junjun Chen, Yirao Lai

et al.

Oral Diseases, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

To investigate the effects of heat shock protein 90 (HSP90) complex from oral lichen planus (OLP) lesion tissues on dendritic cell (DC) activation and polarization naïve T cells.

Language: Английский

Citations

1
The heat shock protein 90 inhibitor RGRN-305 attenuates SARS-CoV-2 spike protein-induced inflammation in vitro but lacks effectiveness as COVID-19 treatment in mice DOI Creative Commons
Hakim Ben Abdallah,

Giorgia Marino,

Manja Idorn

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(9), P. e0310915 - e0310915

Published: Sept. 26, 2024

The inhibition of heat shock protein 90 (HSP90), a molecular chaperone, has been proposed to be potential novel treatment strategy for Coronavirus disease 2019 (COVID-19). In contrast other studies, our data demonstrated that RGRN-305, HSP90 inhibitor, exacerbated the cytopathic effect and did not reduce viral shedding in VeroE6-hTMPRSS2 cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Likewise murine model SARS-CoV-2, transgenic mice treated orally RGRN-305 exhibited reduced survival by end experiment (day 12) as 14% (1/7) survived compared 63% (5/8) those drug-vehicle. Animal weight was treatment. Interestingly, we significantly dampened inflammatory response induced SARS-CoV-2 spike human macrophage-like (U937) lung epithelial (A549). Measured quantitative real-time PCR, mRNA expression proinflammatory cytokines TNF , IL1B IL6 were reduced. Together, these suggest exacerbates infection vitro reduces but exhibits strong anti-inflammatory properties. This shows while may helpful ‘cytokine storm’, it no beneficial impact on replication or animals monotherapy. Further animal studies inhibitors combination an anti-viral drug provide additional insights into its utility infections whether continue COVID-19 disease.

Language: Английский

Citations

0
Association of inflammatory skin biomarkers with clinical response in RGRN‐305‐treated patients with hidradenitis suppurativa DOI
Hakim Ben Abdallah, Anne Bregnhøj, Thomas Emmanuel

et al.

Journal of the European Academy of Dermatology and Venereology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Data will be shared upon reasonable request to the corresponding author.

Language: Английский

Citations

0
Investigation of catechin’s anti-inflammatory activity: A bioinformatics and molecular docking study DOI Creative Commons
Nisa Yulianti Suprahman,

Bulan Rosita Sari,

Riri Fauziyya

et al.

Acta Biochimica Indonesiana, Journal Year: 2024, Volume and Issue: 7(2), P. 199 - 199

Published: Dec. 28, 2024

Background: Inflammation plays a key role in the progression of many chronic diseases. As country with rich biodiversity, Indonesia offers numerous phytochemicals potential for drug development, including catechin, natural compound anti-inflammatory properties. Objective: This study aimed to identify targets catechin and evaluate its inhibitory potency through molecular docking simulations. Methods: Data acquisition refinement were conducted using NCBI, STRING, STITCH databases, intersections identified Venn diagrams. Molecular was performed AutoDockTools 1.5.6, interactions visualized BIOVIA Discovery Studio. Results: Bioinformatics analysis predicted that inhibits three pro-inflammatory proteins: COX-2, HSP90, IL-2. Catechin’s indicated by negative binding energies amino acid residues critical target protein activity. Among targets, IL-2 exhibited lowest energy (-5.12 kcal/mol), suggesting it as primary target. However, catechin’s affinity lower than native ligand (-11.78 kcal/mol). Conclusion: is be Structural modifications are recommended enhance therapeutic potential.

Language: Английский

Citations

0