Journal of Proteome Research,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 13, 2024
Antibodies
have
a
key
role
in
the
immune
system,
making
their
characterization
essential
to
biomedical,
biopharmaceutical,
and
clinical
research
questions.
Antibody
effector
functions
are
mainly
controlled
by
quantity,
subclass,
Fc
glycosylation.
We
describe
an
integrated
method
measure
these
three
critical
dimensions
simultaneously.
The
subclass-specific
immunoglobulin
G
(IgG)
glycosylation
analysis
combines
immunosorbance
with
glycopeptide-centered
LC-MS
detection.
For
IgG1-specific
quantitation,
commercial,
stable
isotope
labeled
IgG1
protein
standard
was
spiked
into
immunosorbent
eluates.
Robust
quantitation
achieved,
relying
on
combination
of
proteotypic
peptide
most
abundant
glycopeptides,
generated
through
proteolytic
cleavage
from
mixture
natural
recombinant
standard.
Method
performance
demonstrated
large
coronavirus
vaccination
cohort
at
throughput
100
samples/day.
LC-MS-derived,
anti-SARS-CoV-2
spike
concentrations
ranged
10000
ng/mL
correlated
well
clinically
relevant
immunoassay.
Technical
variation
200
times
lower
than
biological
variation;
intermediate
precision
44%.
In
conclusion,
we
present
capable
robustly
simultaneously
assessing
antigen-specific
IgG
studies.
This
will
facilitate
broader
understanding
responses,
especially
important
interplay
among
dimensions.
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: April 12, 2025
A
promising
strategy
in
cancer
immunotherapy
is
activation
of
immune
signalling
pathways
through
antibodies
that
target
co-stimulatory
receptors.
hIgG2,
one
four
human
antibody
isotypes,
known
to
deliver
strong
agonistic
activity,
and
modification
hIgG2
hinge
disulfides
can
influence
immune-stimulating
activity.
This
was
shown
for
directed
against
the
hCD40
receptor,
where
cysteine-to-serine
exchange
mutations
caused
changes
conformational
flexibility.
Here
we
demonstrate
principles
increasing
agonism
by
restricting
conformation
disulfide
be
translated
receptor
h4-1BB,
another
member
tumour
necrosis
factor
superfamily.
Furthermore,
explore
structure-guided
design
anti-hCD40
ChiLob7/4
show
engineering
additional
between
opposing
F(ab')
arms
elicit
restriction,
concomitant
with
enhanced
agonism.
These
results
support
a
mode
subtle
increases
rigidity
significant
improvements
immunostimulatory
thus
providing
rational
more
powerful
therapeutics.
Allergy,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 27, 2024
Abstract
Type
I
hypersensitivity,
also
known
as
classical
allergy,
is
mediated
via
allergen‐specific
IgE
antibodies
bound
to
type
FcR
(FcεRI)
on
the
surface
of
mast
cells
and
basophils
upon
cross‐linking
by
allergens.
This
IgE‐mediated
cellular
activation
may
be
blocked
IgG
through
multiple
mechanisms,
including
direct
neutralization
allergen
or
engagement
inhibitory
receptor
FcγRIIb
which
blocks
signal
transduction.
In
addition,
co‐engagement
FcεRI
IgE‐IgG‐allergen
immune
complexes
causes
down
regulation
receptor‐bound
IgE,
resulting
in
desensitization
cells.
Both,
are
driven
allergen‐binding.
Here
we
delineate
distinct
roles
antibody
affinity
versus
avidity
driving
these
processes
discuss
role
subclasses
inhibiting
basophil
cell
activation.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 24, 2024
Abstract
Hybrid
nanostructures
between
biomolecules
and
inorganic
nanomaterials
constitute
a
largely
unexplored
field
of
research,
with
the
potential
for
novel
applications
in
bioimaging,
biosensing,
nanomedicine.
Developing
such
relies
critically
on
understanding
dynamical
properties
nano–bio
interface.
This
work
introduces
validates
strategy
to
predict
atom‐scale
interactions
water‐soluble
gold
nanoclusters
(AuNCs)
set
blood
proteins
(albumin,
apolipoprotein,
immunoglobulin,
fibrinogen).
Graph
theory
neural
networks
are
utilized
strengths
AuNC–protein
complexes
coarse‐grained
level,
which
then
optimized
Monte
Carlo‐based
structure
search
refined
atomic‐scale
structures.
The
training
data
is
based
extensive
molecular
dynamics
(MD)
simulations
complexes,
validating
MD
show
robustness
predictions.
can
be
generalized
any
provided
that
one
generates
enough
about
interactions,
bioactive
parts
nanostructure
rationally.
