Bioprinting of Cells, Organoids and Organs-on-a-Chip Together with Hydrogels Improves Structural and Mechanical Cues

Cells, Journal Year: 2024, Volume and Issue: 13(19), P. 1638 - 1638

Published: Oct. 1, 2024

The 3D bioprinting technique has made enormous progress in tissue engineering, regenerative medicine and research into diseases such as cancer. Apart from individual cells, a collection of organoids, can be printed combination with various hydrogels. It hypothesized that will even become promising tool for mechanobiological analyses organoids their matrix environments highly defined precisely structured environments, which the mechanical properties cell environment individually adjusted. Mechanical obstacles or bead markers integrated bioprinted samples to analyze deformations forces within these constructs, perform biophysical analysis complex systems, are still not standard techniques. review highlights advances 4D printing technologies integrating cues so next step detailed key future directions organoid generation development disease model regeneration drug testing perspective. Finally, hydrogels, pure natural synthetic hydrogels mixtures, organoid–cell co-cultures, organ-on-a-chip systems organoid-organ-on-a chip combinations introduces use assembloids determine mutual interactions different types cell–matrix interferences specific biological environments.

Language: Английский

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The Role of Cancer Organoids in Ferroptosis, Pyroptosis, and Necroptosis: Functions and Clinical Implications

Biomolecules, Journal Year: 2025, Volume and Issue: 15(5), P. 659 - 659

Published: May 2, 2025

The enduring prevalence of cancer worldwide constitutes a significant public health challenge, thereby emphasizing the imperative for development therapeutic models capable accounting heterogeneity inherent in tumors. In this context, organoids have emerged as powerful tools studying tumor biology, providing valuable insights into complex interactions within microenvironment. Concurrently, research is increasingly focused on non-apoptotic forms regulated cell death (RCD)—including ferroptosis, pyroptosis, and necroptosis—which exert pivotal influences progression. Cancer not only recapitulate genetic phenotypic original tumors but also enable more precise investigations roles RCDs oncology. This review explores utility delineating molecular mechanisms underlying their implications biology treatment responses. By synthesizing recent findings, it highlights essential role organoid uncovering intricate details RCDs. Furthermore, emphasizes promising directions future that aim to deepen our understanding these pathways potential. integration necroptosis provides novel oncogenic facilitates targeted strategies. bridging with human pathophysiology, approach transformative framework dissecting enables design precision therapeutics selectively target machinery

Language: Английский

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Impact of enzymatic isolation on the propagation efficiency of patient-derived colorectal cancer organoids

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 18, 2025

Language: Английский

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Potential Use of Organoids in Regenerative Medicine

Tissue Engineering and Regenerative Medicine, Journal Year: 2024, Volume and Issue: 21(8), P. 1125 - 1139

Published: Oct. 16, 2024

Language: Английский

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Advancements in Microphysiological Systems: Exploring Organoids and Organ-on-a-Chip Technologies in Drug Development -Focus on Pharmacokinetics Related Organs-

Drug Metabolism and Pharmacokinetics, Journal Year: 2024, Volume and Issue: 60, P. 101046 - 101046

Published: Dec. 17, 2024

This study explored the evolving landscape of Microphysiological Systems (MPS), with a focus on organoids and organ-on-a-chip (OoC) technologies, which are promising alternatives to animal testing in drug discovery. MPS technology offers vitro models high physiological relevance, simulating organ function for pharmacokinetic studies. Organoids composed 3D cell aggregates OoCs mimicking vivo environments based microfluidic platforms represent forefront MPS. paper provides comprehensive overview their application studying gut, liver, kidney challenges becoming reliable models. Although is not yet fully comparable systems, its continued development, aided by silico, automation, AI approaches, anticipated bring about further advancements. Collaboration across multiple disciplines ongoing regulatory discussions will be crucial driving toward practical ethical applications biomedical research development.

Language: Английский

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Organoid as a promising tool for primary liver cancer research: a comprehensive review

Cell & Bioscience, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 27, 2024

Abstract Primary liver cancer (PLC) is one of the most common malignant gastrointestinal tumors worldwide. Limited by shortage transplantation donors and heterogeneity tumors, patients with lack effective treatment options, which leads to rapid progression metastasis. Currently, preclinical models PLC fall short clinical reality are limited in their response disease effectiveness drug therapy. Organoids vitro three-dimensional cultured a high degree that preserve histomorphological genomic features primary tumors. Liver organoids have been widely used for screening, new target discovery, precision medicine; thus representing promising tool study PLC. Here, we summarize progress research on potential application as models. This review provides comprehensive introduction this emerging technology offers ideas researchers explore field medicine.

Language: Английский

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Organoid modeling meets cancers of female reproductive tract

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: Sept. 27, 2024

Language: Английский

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Developing biotechnologies in organoids for liver cancer

Biomedical Technology, Journal Year: 2024, Volume and Issue: 9, P. 100067 - 100067

Published: Nov. 26, 2024

Language: Английский

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