Emerging roles of tRNA-derived small RNAs in injuries DOI Creative Commons
Mengjun Wang, Junfeng Guo, Wei Chen

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e18348 - e18348

Published: Oct. 24, 2024

tRNA-derived small RNAs (tsRNAs) are a novel class of noncoding RNAs, precisely cleaved from tRNA, functioning as regulatory molecules. The topic tsRNAs in injuries has not been extensively discussed, and studies on entering new era. Here, we provide fresh perspective this topic. We systematically reviewed the classification, generation, biological functions response to stress, well their potential biomarkers therapeutic targets various injuries, including lung injury, liver renal cardiac neuronal vascular skeletal muscle skin injury. also provided association between stress-induced organ injury clinical perspective.

Language: Английский

Apoptotic Bodies Derived from Fibroblast‐Like Cells in Subcutaneous Connective Tissue Inhibit Ferroptosis in Ischaemic Flaps via the miR‐339‐5p/KEAP1/Nrf2 Axis DOI Creative Commons

Gaoxiang Yu,

Yijie Chen, Ningning Yang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(24)

Published: April 19, 2024

Abstract Preventing and treating avascular necrosis at the distal end of flaps are critical to surgery success, but current treatments not ideal. A recent study shows that apoptotic bodies (ABs) generated near site apoptosis can be taken up promote cell proliferation. The reveals ABs derived from fibroblast‐like cells in subcutaneous connective tissue (FSCT cells) skin promoted ischaemic flap survival. It is also found inhibited death oxidative stress M1‐to‐M2 polarization macrophages. Transcriptome sequencing protein level testing demonstrated survival endothelial macrophages by inhibiting ferroptosis via KEAP1‐Nrf2 axis. Furthermore, microRNA (miR) data vitro vivo experiments KEAP1 delivering miR‐339‐5p exert therapeutic effects. In conclusion, FSCT cell‐derived ferroptosis, macrophage transition miR‐339‐5p/KEAP1/Nrf2 axis These results provide a potential strategy administering ABs.

Language: Английский

Citations

18
Mechanisms of radiation‐induced tissue damage and response DOI Creative Commons

Zhou Lin,

Jiaojiao Zhu, Yuhao Liu

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(10)

Published: Sept. 20, 2024

Radiation-induced tissue injury (RITI) is the most common complication in clinical tumor radiotherapy. Due to heterogeneity response of different tissues radiation (IR), radiotherapy will cause types and degrees RITI, which greatly limits application Efforts are continuously ongoing elucidate molecular mechanism RITI develop corresponding prevention treatment drugs for RITI. Single-cell sequencing (Sc-seq) has emerged as a powerful tool uncovering mechanisms identifying potential targets by enhancing our understanding complex intercellular relationships, facilitating identification novel cell phenotypes, allowing assessment spatiotemporal developmental trajectories. Based on comprehensive review we analyzed regulatory networks combination with Sc-seq summarized targeted intervention pathways therapeutic Deciphering diverse underlying can shed light its pathogenesis unveil new avenues potentially facilitate repair or regeneration currently irreversible Furthermore, discuss how personalized strategies based offer promise mitigating

Language: Английский

Citations

5
The Proimmunomodulatory and Anti-immunomodulatory Effects of Radiotherapy in Oncologic Care DOI

Bryan Johnson,

Qianyu Guo,

Keyur Chaludiya

et al.

Hematology/Oncology Clinics of North America, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
The Extra‐Tumoral Vaccine Effects of Apoptotic Bodies in the Advancement of Cancer Treatment DOI Creative Commons
Yulian Wang, Chunyan Liu, Jiayun Pang

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Abstract The induction of apoptosis in tumor cells is a common target for the development anti‐tumor therapies; however, these therapies still leave patients at increased risk disease recurrence. For example, apoptotic can promote growth and immune evasion via secretion metabolites, extracellular vesicles, pro‐tumorigenic macrophages. This paradox effects cell has begged question whether suitable cancer therapy, led to further explorations into other immunogenic death‐based approaches. However, strategies face multiple challenges, most critical which microenvironment. Contrary promotion tolerance mediated by cells, bodies with enriched tumor‐related antigens have demonstrated great potential, as evidenced their ability initiate systemic T‐cell responses. These characteristics indicate that body‐based could be ideal “in situ” extra‐tumoral vaccine candidates treatment cancers, address current issues apoptosis‐based or immunotherapy treatments. Although not yet tested clinically, vaccines potential better patient outcomes future.

Language: Английский

Citations

0
Gancao Xiexin decoction attenuated experimental colitis through suppressing ACSL4-mediated ferroptosis DOI

Zengfeng Pan,

Caiyan Gan,

Shaobin Zhi

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119532 - 119532

Published: Feb. 1, 2025

Language: Английский

Citations

0
Engineering cuttlefish melanin nanoparticles: A dual-action therapy for acute radiation syndrome and combined radiation-wound injuries via regulating Bcl-2 family proteins and caspases in the apoptotic process DOI

Zhemeng Zhao,

Bin Hu,

Danfeng Zhang

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162108 - 162108

Published: March 1, 2025

Language: Английский

Citations

0
Microbiome and Inflammasome Alterations Found During Radiation Dose Finding in a Sinclair Minipig Model of Gastrointestinal Acute Radiation Syndrome DOI

Timothy S. Horseman,

Babita Parajuli,

Andrew M. Frank

et al.

Shock, Journal Year: 2024, Volume and Issue: 62(4), P. 556 - 564

Published: July 16, 2024

ABSTRACT Both abdominal radiotherapy and a nuclear event can result in gastrointestinal symptoms, including acute radiation syndrome (GI-ARS). GI-ARS is characterized by compromised intestinal barrier integrity increasing the risk for infectious complications. Physiologically relevant animal models are crucial elucidating host responses therapeutic targets. We aimed to determine dose requirements creating Sinclair minipig. Male, sexually mature swine were randomly divided into sham (n = 6) three lower hemibody dosage groups of 8, 10, 12 Gy 5/group) delivered using linear accelerator-derived x-rays (1.9 Gy/min). Animals monitored symptoms 14 days with rectal swab blood collection at 0–3, 7, followed necropsy western blotting histology. Dose-dependent increases weight loss, diarrhea severity, mortality (log-rank test, P 0.041) seen. Villi length was significantly reduced all irradiated animals compared controls ( < 0.001). Serum citrulline decreased bacterial translocation increased after irradiation controls. Increased NLRP3 levels post-mortem jejunum seen 0.0043) as well IL-1β group 0.041). Radiation survival associated significant gut microbial community shifts beta diversity. Moreover, decedents had Porphyromonas, Campylobacter, Bacteroides , Parvimonas Fusobacterium Aerococcus, Lactobacillus, Prevotella, Streptococcus . Our novel minipig model showed dose-dependent clinical GI-ARS. These findings provide invaluable insights intricate interplay between GI-ARS, inflammation, microbiota alterations offering potential targets diagnostic interventions exposure.

Language: Английский

Citations

2
A comprehensive review of sensors of radiation‐induced damage, radiation‐induced proximal events, and cell death DOI Creative Commons
Saurabh Saini, Prajwal Gurung

Immunological Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 19, 2024

Summary Radiation, a universal component of Earth's environment, is categorized into non‐ionizing and ionizing forms. While radiation relatively harmless, possesses sufficient energy to ionize atoms disrupt DNA, leading cell damage, mutation, cancer, death. The extensive use radionuclides in nuclear technology medical applications has sparked global concern for their capacity cause acute chronic illnesses. Ionizing induces DNA damage either directly through strand breaks base change or indirectly by generating reactive oxygen species (ROS) nitrogen (RNS) via radiolysis water. This triggers complex cellular response involving recognition cycle arrest, repair mechanisms, release pro‐inflammatory cytokines, review focuses on the mechanisms radiation‐induced subsequent activation processes, critical role innate immune resolution injury. Emphasis placed pattern receptors (PRRs) related that detect damage‐associated molecular patterns (DAMPs) initiate downstream signaling pathways. Radiation‐induced death pathways are discussed detail. Understanding these processes crucial developing strategies mitigate harmful effects improve therapeutic outcomes.

Language: Английский

Citations

2
Combination Immunotherapy With Radiotherapy in Non‐Small Cell Lung Cancer: A Review of Evidence DOI Creative Commons
Justin Burr, Kurtis C. Johnson, Joseph Carmicheal

et al.

Cancer Medicine, Journal Year: 2024, Volume and Issue: 13(21)

Published: Nov. 1, 2024

ABSTRACT Background Radiotherapy plays a fundamental role in the treatment of patients with all stages non‐small‐cell lung cancer (NSCLC). The emergence immune checkpoint inhibitors (ICIs) has transformed standard care these patients. use ICIs is increasingly utilized definitive setting as an adjunct to chemoradiotherapy or surgery and remains vital component metastatic disease. Despite improvements patient survival, immunotherapy monotherapy shown limited overall response rates susceptibility resistance. been identified viable option enhance rate ICI improve outcomes NSCLC. Methods We queried English PubMed database utilizing variably combined search items including “radiation,” “chemoradiation,” “immune checkpoint,” “immunotherapy,” “stereotactic body radiotherapy,” “non‐small‐cell lung”. additionally searched various acceptable alternative terms for similar keywords such “radiotherapy” place “radiation.” These results were subsequently curated relevance impact on current paradigms. Results In this review, we discuss preclinical clinical studies relating combinatorial radiation are presented context early‐stage, operable stage III, unresectable majority data illustrate promising regarding additive synergistic effects suggestion that timing modalities crucial optimizing outcomes. Conclusion While there now evidence favorable interplay between NSCLC, remain multiple unanswered questions which expected be addressed ongoing trials.

Language: Английский

Citations

2
Radiation-induced eCIRP impairs macrophage bacterial phagocytosis DOI
Satoshi Yamaga,

Atsushi Murao,

Mian Zhou

et al.

Journal of Leukocyte Biology, Journal Year: 2024, Volume and Issue: 116(5), P. 1072 - 1079

Published: June 26, 2024

Abstract Macrophages are essential immune cells for host defense against bacterial pathogens after radiation injury. However, the role of macrophage phagocytosis in infection following injury remains poorly examined. Extracellular cold-inducible RNA-binding protein is a damage-associated molecular pattern that dysregulates system responses such as phagocytosis. We hypothesized radiation-induced extracellular release impairs bacteria. Adult healthy mice were exposed to 6.5 Gy total body irradiation. Primary peritoneal macrophages isolated from adult radiation. protein–neutralizing monoclonal antibody was added cell culture prior Bacterial by assessed using pHrodo Green-labeled Escherichia coli 7 d irradiation ex vivo and vitro. also treatment with recombinant murine protein. Rac1 ARP2 expression lysates levels lavage western blotting. significantly decreased compared controls downregulated Total increased cavity. Recombinant dose-dependent manner. restored Ionizing exposure Neutralization restores phagocytic ability Our findings elucidate novel mechanism dysfunction provide potential new therapeutic approach limiting

Language: Английский

Citations

1