Compensatory mechanisms in γδ T cell-deficient chickens following Salmonella infection
Frontiers in Immunology,
Journal Year:
2025,
Volume and Issue:
16
Published: May 14, 2025
Avian
γδ
T
lymphocytes
are
highly
abundant
in
the
intestinal
mucosa
and
play
a
critical
role
immune
defense
against
infectious
diseases
chickens.
However,
their
specific
contributions
to
infection
control
remain
poorly
understood.
To
investigate
of
cells
possible
compensation,
we
studied
wild-type
cell
knockout
chickens
following
with
Salmonella
Enteritidis.
Bacterial
loads
liver,
cecal
content,
wall
were
quantified.
Immune
populations
blood,
spleen,
cecum
analyzed
using
flow
cytometry.
gene
transcription
sorted
(TCR1
+
)
TCR1
-
subsets
as
well
tissue
was
measured
by
RT-qPCR.
Strikingly,
lacking
had
significantly
higher
bacterial
liver
more
extensive
invasion
during
early
stages
compared
birds.
In
infected
displayed
increased
percentage
CD25
NK-like
cells.
both
blood
tissue,
demonstrated
an
absolute
number
CD8αα
+hi
(CD4
).
Conversely,
exhibited
augmented
count
CD4
population
after
infection,
which
might
include
αβ
At
7
days
post
(dpi),
expression
analysis
revealed
elevated
activation
marker
IL-2Rα
proinflammatory
cytokines
(IL-17A,
IFN-γ)
from
By
12
dpi,
these
differences
diminished
levels
animals.
Our
findings
demonstrate
that
protection
Enteritidis
later
functions
appear
be
replaced
other
Language: Английский
Tributyltin (TBT) toxicity: Effects on enteric neuronal plasticity and intestinal barrier of rats' duodenum
I C C S Oliveira,
No information about this author
Gabriela Pustiglione Marinsek,
No information about this author
Lucas Vilas Bôas Correia
No information about this author
et al.
Autonomic Neuroscience,
Journal Year:
2024,
Volume and Issue:
253, P. 103176 - 103176
Published: April 18, 2024
Language: Английский
The Bacterial Cell Wall Components Lipopolysaccharide and Peptidoglycan Initiate Divergent Local Tissue and Systemic Inflammatory Response Profiles in the Chicken Model
Animals,
Journal Year:
2024,
Volume and Issue:
14(24), P. 3661 - 3661
Published: Dec. 18, 2024
The
innate
immune
system
plays
an
important
role
in
the
defense
against
pathogens,
whereby
ability
to
rapidly
mount
effective
inflammatory
response
is
critical
elimination/containment
of
infection.
To
better
understand
nature
responses
bacterial
components
chickens,
we
used
growing
feather
(GF)
cutaneous
bioassay
together
with
blood
sampling
examine
local
and
systemic
initiated
by
intradermal
(i.d.)
GF-pulp
injection
lipopolysaccharide
(LPS)
from
Salmonella
Typhimurium
or
peptidoglycan
(PGN)
Staphylococcus
aureus.
Three
studies
were
conducted
egg-type
chickens
between
9
15
weeks
age;
Study
1
2
examined
leukocyte
profiles
a
100-fold
dose
range
LPS
PGN
over
24
h
7
d,
respectively;
3
leukocyte-
cytokine
mRNA-profiles
pulps
concurrently
72
h.
I.d.
stimulated
heterophil
monocyte/macrophage
dominated
both
GF-pulps
that
was
resolved
48–72
differed
based
on
administered.
characterized
rapid
infiltration
lymphocytes
sustained
high
levels
T
B
cells
5–7
d
neither
affected
nor
reflected
blood.
Limited
transcriptome
analyses
did
not
reveal
differences
could
explain
divergent
versus
PGN.
More
research
needed
mechanisms
underlying
chickens.
Language: Английский
Dynamic Changes in the Intraepithelial Lymphocyte Numbers Following Salmonella Typhimurium Infection in Broiler Chickens
Animals,
Journal Year:
2024,
Volume and Issue:
14(23), P. 3463 - 3463
Published: Nov. 30, 2024
At
day
21
of
age,
Ross-308
broilers
were
orally
gavaged
with
7.5
×
10
Language: Английский