hAMSCs regulate EMT in the progression of experimental pulmonary fibrosis through delivering miR-181a-5p targeting TGFBR1

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 5, 2025

Pulmonary fibrosis (PF) is a common and multidimensional devastating interstitial lung disease. The development of novel more effective interventions for PF an urgent clinical need. A previous study has found that miR-181a-5p plays important role in the PF, human amniotic mesenchymal stem cells (hAMSCs) exert potent therapeutic potential on PF. However, whether hAMSCs act by delivering its detailed mechanism still remain unknown. Thus, this was designed to investigate underlying possible bleomycin (BLM)-induced mouse model, co-culture system A549 epithelial transition (EMT) focusing effects collagen deposition, EMT, cell cycle regulation. with different expression levels were constructed. BLM (4 mg/kg) used create while TGF-β1 induce construct EMT model. Furthermore, deposition during assessed vivo vitro. We exerted anti-fibrotic effect BLM-induced Moreover, also protective TGFβ1-induced ameliorated promoting proliferation, reducing apoptosis, attenuating through paracrine effects. regulated targeting TGFBR1. Our findings reveal first time inhibit EMT. Mechanistically, hMASCs achieved

Language: Английский

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Recent advances in mesenchymal stem cell therapy for multiple sclerosis: clinical applications and challenges

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 3, 2025

Multiple sclerosis (MS), a chronic autoimmune disorder of the central nervous system (CNS), is characterized by inflammation, demyelination, and neurodegeneration, leading to diverse clinical manifestations such as fatigue, sensory impairment, cognitive dysfunction. Current pharmacological treatments primarily target immune modulation but fail arrest disease progression or entirely reverse CNS damage. Mesenchymal stem cell (MSC) therapy offers promising alternative, leveraging its immunomodulatory, neuroprotective, regenerative capabilities. This review provides an in-depth analysis MSC mechanisms action, including regulation, promotion remyelination, neuroregeneration. It examines preclinical studies trials evaluating efficacy, safety, limitations in various MS phenotypes. Special attention given challenges delivery routes, dosing regimens, integrating MSCs with conventional therapies. By highlighting advancements ongoing challenges, this underscores potential revolutionize treatment, paving way for personalized combinatory therapeutic approaches.

Language: Английский

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Stem cell therapy for the treatment of psychiatric disorders: a real hope for the next decades

Frontiers in Psychiatry, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

In this review, it is evaluated the progress in application of stem cell therapy to ameliorate symptoms bipolar disorder, major depression, schizophrenia, and autism. These disorders are highly prevalent clinical medicine responsible for high levels psychosocial disability among patients. All them share common biomedical features, such as complex variable genetic substrates, significant susceptibility environmental changes, insufficient knowledge their pathogenesis. addition, responsiveness patients pharmacological treatment heterogeneous, some cases, no available. Therefore, development cell-based regenerative its possible combination with emerging therapeutic approaches that promote neural plasticity expected advance neuropsychiatry next few decades.

Language: Английский

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Vitronectin Regulates Focal Adhesion Turnover and Migration of Human Placenta-derived MSCs under Nutrient Stress

European Journal of Cell Biology, Journal Year: 2025, Volume and Issue: unknown, P. 151477 - 151477

Published: Jan. 1, 2025

Language: Английский

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Targeted cytokine delivery for cancer therapy through engineered mesenchymal stem cells

Gene Reports, Journal Year: 2025, Volume and Issue: unknown, P. 102199 - 102199

Published: March 1, 2025

Language: Английский

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Intraosseous Delivery of Mesenchymal Stem Cells for the Treatment of Bone and Hematological Diseases

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(11), P. 12672 - 12693

Published: Nov. 8, 2024

Mesenchymal stem cells are used most in regenerative medicine due to their capacities differentiation and immune modulation. The intraosseous injection of MSC into the bone has been recommended because expected outcomes for retention, bioavailability, enhanced therapeutic efficacy, particularly conditions involving bone, such as osteoporosis osteonecrosis. A review delivery mesenchymal comparison with intravenous intra-arterial methods will be subjected critical examination. This mode fares better regarding paracrine signaling immunomodulation attributes, which cornerstone tissue regeneration inflammation reduction. local complications technical challenges still apply this method. study was more focused on further research soon conducted elucidate long-term safety efficacy cell therapy. Though much achieved very impressive progress field, it is worth noting that studies need put place so technique can established a routine approach, especially biomaterials, gene therapy, personalized medicine.

Language: Английский

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Laboratory investigation of METTL7A driving MSC osteogenic differentiation through YAP1 translation enhancement via eIF4F recruitment

International Endodontic Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Effective control of mesenchymal stem cell (MSC) differentiation towards osteogenic lineages is fundamental for bone regeneration. This study elucidates the regulatory role methyltransferase like 7A (METTL7A) in MSCs. Alkaline phosphatase staining, Alizarin Red S western blotting, and vivo studies were conducted to determine effects METTL7A depletion or overexpression on various types Then downstream signalling pathways regulated by MSCs further investigated. Our findings indicate that expression significantly increases during Furthermore, hindered, whereas its enhanced, Mechanistically, influences MSC activating YAP1-TEAD1 pathway. It enhances YAP1 not only stabilising mRNA but also, crucially, recruiting eIF4F complex, thereby boosting translation efficiency mRNA. Additionally, YAP1/TEAD1 complex transcriptionally regulates expression, creating a positive feedback loop amplifies differentiation. Overall, our uncovers previously unknown molecular mechanism suggests could offer new avenues enhancing

Language: Английский

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Two promising approaches in the treatment of myocardial infarction: stem cells and gene therapy

Frontiers in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 19, 2025

Cardiovascular diseases (CVDs), characterized by a high incidence rate and mortality, have become the leading cause of death globally. CVDs include coronary heart disease, stroke, hypertension, peripheral vascular diseases. In China, ranks first in all major At present, main methods to treat ischemic disease are drug therapy, intervention operation. These only alleviate symptoms failure myocardial ischemia improve patients' quality life partially restoring reperfusion. Due extensive irreversible necrosis cells caused hypoxia, these cannot reverse damage, resulting suboptimal long-term outcomes. Although mature cardiomyocytes been proved not be terminally differentiated cells, they very limited ability regeneration proliferation, so can completely replace damaged myocardium restore contractile function. transplantation heart, its clinical application promotion source donor, expensive cost, immune rejection, ethical problems. It has an urgent task for medicine seek new better treatment. The content this paper is explore stem gene technology treatment infarction (MI).

Language: Английский

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Emerging role of mesenchymal cells in cardiac and cerebrovascular diseases: Physiology, pathology, and therapeutic implications

Vascular Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 107473 - 107473

Published: Feb. 1, 2025

Language: Английский

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Measurement of Oxygen Transfer Rate and Specific Oxygen Uptake Rate of h-iPSC Aggregates in Vertical Wheel Bioreactors to Predict Maximum Cell Density Before Oxygen Limitation

Bioengineering, Journal Year: 2025, Volume and Issue: 12(4), P. 332 - 332

Published: March 22, 2025

The prediction of the cell yield in large-scale bioreactor culture is an important factor for various therapy bioprocess operations to ensure consistency quality and efficient use resources. However, shear sensitivity cells used manufacturing can make such predictions difficult, particularly suspension cultures that have significant stresses without representative scale down models. PBS Vertical-Wheel (VW) bioreactors been demonstrated provide a homogeneous hydrodynamic environment with low at scales (0.1–80 L) thereby employed shear-sensitive cells. In this study, oxygen transfer rate surface aeration three VW was measured along specific uptake (sOUR) iPSCs cultured bioreactors. mass coefficient PBS-3/15/80 L different agitation rates, headspace gas flowrates, working volumes using static gassing-out method. sOUR dynamic method PBS-0.1 Mini custom DO probe configuration. results from both experiments were combined calculate theoretical maximum density before limitation across 2 L/3 L/10 L/15 L/50 L/80 speed rate.

Language: Английский

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