Oral Microbiome Research in Biopsy Samples of Oral Potentially Malignant Disorders and Oral Squamous Cell Carcinoma and Its Challenges DOI Creative Commons
Bruno Špiljak, Petar Ozretić, Ana Andabak Rogulj

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(23), P. 11405 - 11405

Published: Dec. 7, 2024

This study aims to evaluate the potential benefits and challenges of integrating oral microbiome research into clinical management potentially malignant disorders (OPMD) squamous cell carcinoma (OSCC). The has gained significant attention for its role in pathogenesis progression these conditions, with emerging evidence suggesting value as a diagnostic prognostic tool. By critically analyzing current methodological considerations, this manuscript examines whether analysis biopsy samples can aid early detection, prognosis, OPMD OSCC. complexity dynamic nature require multifaceted approach fully understand utility. Based on review, we conclude that studying context holds promise but also faces notable challenges, including variability need standardization. Ultimately, addresses question, “Should such be undertaken, given intricate interactions various factors inherent obstacles involved?”, emphasizes importance further optimize applications improve patient outcomes.

Language: Английский

Oral Microbiota and Inflammatory Bowel Diseases: Detection of Emerging Fungal Pathogens and Herpesvirus DOI Creative Commons
Manoel Marques Evangelista Oliveira, Letícia Campos, Fernanda Brito

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 480 - 480

Published: Feb. 15, 2025

Background/Objectives: Ulcerative colitis (UC) and Crohn’s disease (CD) are the usual clinical forms of inflammatory bowel (IBD). Changes in oral microbiota, especially presence emerging fungi herpesviruses, have been shown to worsen aspects IBD. The aim this study was screen for pathogens yeast microbiota herpesvirus IBD patients. Methods: Oral swabs seven UC or CD patients were collected. samples plated on Sabouraud Dextrose Agar subcultured CHROMagar Candida Plus. Polyphasic taxonomy applied identified using molecular tools, such as MALDI-TOF MS ITS partial sequencing. Multiplex qPCR used identify herpesvirus. Results: mean age 38.67 ± 14.06 years, 57.14% female, two had diabetes. presented with Rhodotorula mucilaginosa, orthopsilosis Kodamaea jinghongensis, while Cutaneotrichosporon dermatis, glabrata, lusitanea tropicalis. Two individuals at least one In first individual, a co-detection Herpes Simplex Virus 1 (HSV-1) C. lusitaniae observed. second co-infections Epstein–Barr virus (EBV), Human Herpesvirus 7 (HHV-7) Conclusions: We rarely described yeasts patients, highlighting need they may contribute opportunistic infections.

Language: Английский

Citations

0
Oral Fusobacterium nucleatum exacerbates ulcerative colitis via the oral-gut axis: mechanisms and therapeutic implications DOI Creative Commons
Zhili Zheng, Wenqin Jin, Weiwei Guo

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 7, 2025

Fusobacterium nucleatum (F. nucleatum) is an anaerobic bacterium known for its association with periodontal disease and oral infections. It has been implicated in the development of gastrointestinal diseases such as inflammatory bowel colorectal cancer. Ulcerative colitis (UC), which characterized by chronic inflammation colon, a condition unknown etiology rising incidence rate, significantly affecting quality life patients. The increased intestinal permeability during UC may facilitate adherence or invasion F. into damaged barrier, leading to exacerbated inflammation. This article introduces concept oral-gut axis, reviewing existing literature analyze role pathogenesis exploring potential pathogenic mechanisms. also summarizes latest advances treating patients who have looks forward prospective therapeutic strategies translational prospects within axis. be key player UC, likely due invasiveness periods permeability. paper discusses innovative approaches prevention management nucleatum, paving way more effective treatment UC. review offers new insights complex relationship between microbiome diseases, enhancing our understanding their dynamic interactions. There paucity on approaches, indicating need further clinical research.

Language: Английский

Citations

0
The effects of probiotic treatment with Bifidobacterium breve, Bif195 for small intestinal Crohn’s disease and the gut microbiome: results from a randomised, double-blind, placebo-controlled trial DOI Creative Commons

Ida Marie Bruun Grønbæk,

Sofie Ingdam Halkjær, Sarah Mollerup

et al.

Gut Pathogens, Journal Year: 2025, Volume and Issue: 17(1)

Published: April 9, 2025

Language: Английский

Citations

0
Advancing gut microbiome insights: Graphene quantum dot nanobiosensors for microbial metabolite detection DOI
Soheil Sadr,

Shakiba Nazemian,

Shiva Dianaty

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162954 - 162954

Published: April 1, 2025

Citations

0
The Bidirectional Effects of Periodontal Disease and Oral Dysbiosis on Gut Inflammation in Inflammatory Bowel Disease DOI
Netanel Zilberstein, Phillip A. Engen, Garth Swanson

et al.

Journal of Crohn s and Colitis, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Background and Aims Inflammatory bowel disease (IBD) flares can lead to excessive morbidity mortality. This study aimed determine whether oral dysbiosis/periodontal (PD) is common in IBD associated with activity IBD. Methods single-center, prospective, cross-sectional, proof-of-concept, observational assessed the frequency of periodontal inflammatory interrogated stool microbiota using 16S rRNA gene amplicon sequencing active-IBD (aIBD), inactive-IBD (iIBD), healthy controls (HC). Questionnaires diet, alcohol usage, hygiene behavior, activity. A subset participants underwent comprehensive dental examinations evaluate PD. Results Periodontal was severer aIBD subjects than HC, as had poorer quality diets (lower Mediterranean diet scores) iIBD HC. Significant differences microbial community structure were observed unstimulated saliva, stimulated gingiva, samples, primarily between Saliva from higher relative abundances putative pathobionts genera Streptococcus, Granulicatella, Rothia, Actinomyces despite similar behaviors groups. Conclusions Our suggests that patients have disorders ‘pro-inflammatory’ their cavity, normal behaviors. data are consistent potential presence an oral-gut axis could trigger flare-ups at-risk patients. Routine health assessments all should be encouraged part maintenance a strategy decrease risk flares.

Language: Английский

Citations

2
Oral Microbiome Research in Biopsy Samples of Oral Potentially Malignant Disorders and Oral Squamous Cell Carcinoma and Its Challenges DOI Creative Commons
Bruno Špiljak, Petar Ozretić, Ana Andabak Rogulj

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(23), P. 11405 - 11405

Published: Dec. 7, 2024

This study aims to evaluate the potential benefits and challenges of integrating oral microbiome research into clinical management potentially malignant disorders (OPMD) squamous cell carcinoma (OSCC). The has gained significant attention for its role in pathogenesis progression these conditions, with emerging evidence suggesting value as a diagnostic prognostic tool. By critically analyzing current methodological considerations, this manuscript examines whether analysis biopsy samples can aid early detection, prognosis, OPMD OSCC. complexity dynamic nature require multifaceted approach fully understand utility. Based on review, we conclude that studying context holds promise but also faces notable challenges, including variability need standardization. Ultimately, addresses question, “Should such be undertaken, given intricate interactions various factors inherent obstacles involved?”, emphasizes importance further optimize applications improve patient outcomes.

Language: Английский

Citations

1