Vpliv simptomatske bakterijske okužbe na izražanje genov, povezanih z imunostjo v hemocitih kopenskega raka Porcellio scaber DOI Creative Commons

Andraž Dolar,

Jernej Ogorevc, Anita Jemec Kokalj

et al.

Acta Biologica Slovenica, Journal Year: 2023, Volume and Issue: 66(2)

Published: Oct. 5, 2023

Kopenski enakonožci vrste Porcellio scaber so v naravnem okolju izpostavljeni številnim patogenom in parazitom, ki lahko pri gostitelju povzročijo poškodbe tkiv ter vplivajo na imunokompetenco fitnes organizma. Bakterijska okužba povzroči aktivacijo mehanizmov prirojene imunosti, kot fagocitoza, tvorba reaktivnih kisikovih dušikovih zvrsti, aktivnost antioksidativnih encimov, nodulacija proces melanizacije. Molekularni vzorci patogenov oziroma mikrobov s patogenezo povezane sprožijo prepisovanje genov celicah hemolimfe, tj. hemocitih, opravljajo pomembno funkcijo mediatorjev imunskega odgovora. V aktualni raziskavi smo preučevali spremembe izražanju ob simptomatski bakterijski okužbi z Rhabochlamydia porcellionis jih primerjali asimptomatskimi zdravimi P. scaber. Iz hemolimfe (hemocitov) asimptomatskih simptomatskih živali izolirali celokupno RNA, jo prepisali cDNA metodo RT-qPCR določili relativno izražanje izbranih genov, povezanih imunostjo (Toll4, Dscam, MyD88, Cat, MnSod, CypG, A2m, Atg5 Nos). Ugotovili značilne kar kaže njihovo vlogo imunskem odgovoru primeru bakterijske okužbe, dodatno pa biokemijskimi metodami dokazali povečano encima alfa-2-makroglobulin mejno značilno povišanje katalaza. Na podlagi rezultatov zaključimo, da preučevani geni predstavljajo molekularne označevalce za imunski odziv, je moč uporabiti različnih okoljskih raziskavah.

Integrative Analysis of Hepatopancreas Transcriptome and Proteome in Female Eriocheir sinensis under Thermal Stress DOI Open Access
Tingshuang Pan, Tong Li, Min Yang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7249 - 7249

Published: June 30, 2024

The Chinese mitten crab (

Language: Английский

Citations

2
Tire microplastics exposure in soil induces changes in expression profile of immune-related genes in terrestrial crustacean Porcellio scaber DOI

Andraž Dolar,

Damjana Drobne, Mojca Narat

et al.

Environmental Pollution, Journal Year: 2022, Volume and Issue: 314, P. 120233 - 120233

Published: Sept. 21, 2022

Language: Английский

Citations

10
Race to the poles: the thermal response of the transcriptome of two range-expanding pelagic amphipod species DOI
Diana Martínez-Alarcón, Christoph Held, Lars Harms

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: March 21, 2024

Introduction Hyperiid amphipods of the genus Themisto are a key polar zooplankton group in terms biomass and play an important role as prey for higher trophic levels. They prone to undergo changes abundance distribution course ongoing environmental changes. In Southern Ocean, gaudichaudii is predicted expand its poleward. Arctic, boreal-Atlantic T. abyssorum increases abundance, resulting increased competition with genuine congener. It not known, however, whether have potential efficiently adapt changing water temperatures at their current range or they will be shifting ranges Methods We exposed two species from different geographic populations temperature-change experiments, cold treatment heat-shock treatment. After that, we carried out transcriptome sequencing compare gene expression patterns treatments species. Results show that under similar heat conditions, differentially expressed more genes (26-fold change) than . Furthermore, observed qualitative differences between genetic clusters Discussion The suggest evolutionary divergence can linked regulatory pathways involved temperature stress. These could influence capacity each cluster cope differently contrast congeneric species, showed pronounced adaptive flexibility thermal stress; it appears ability continue poleward expansion but may also increasing environments. Our findings contribute understand response range-shifting stress view gradients encounter throughout future ranges.

Language: Английский

Citations

1
Positive selection in cilia-related genes may facilitate deep-sea adaptation of Thermocollonia jamsteci DOI

Zelin Duan,

Jing Wang, Shuya Liu

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 950, P. 175358 - 175358

Published: Aug. 8, 2024

Language: Английский

Citations

1
Molecular pathways of osmoregulation in response to salinity stress in the gills of the scalloped spiny lobster (Panulirus homarus) within survival salinity DOI

Hongmei Ran,

Zecheng Li, Fengtang Yang

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 52, P. 101308 - 101308

Published: Aug. 10, 2024

Language: Английский

Citations

1
Salinity Fluctuation on the Genetic Regulatory Mechanisms of the Crustacean, Charybdis japonica DOI Creative Commons

Bonian Shui,

Wang Yu-ju,

Fangrui Lou

et al.

Frontiers in Marine Science, Journal Year: 2022, Volume and Issue: 9

Published: July 7, 2022

The intertidal salinity fluctuations provide pose a significant physical challenge for the crustaceans that live here. In present study, Charybdis japonica was selected as research species to explore genetic regulatory mechanisms of responding fluctuations. We cultured C. s with three gradients (the 15‰, 25‰, and 35‰) 68.90 Gb clean transcriptome reads were obtained by RNA-seq. All then de novo assembled 41,058 unigene mean length 1,179.39 bp an N50 2,033 bp. Furthermore, total 18,100 (24.74%) unigenes successfully matched sequences from at least one database. further analyzed structure 26,853 CDSs 12,190 SSRs predicted. gene expression levels 25‰ used control, 204 5,392 DEGs found 35‰ respectively. Based on annotated information DEGs, we speculated japonicas subjected greater stress under isotonic intracellular regulation activated. neuronal activity, oxidation response, activity osmotic regulation-related genes be critical cope results will fundamental revealing fluctuation-related other crustaceans, help us predict how future changes affect survival crustaceans.

Language: Английский

Citations

6
Transcriptome analysis and identification of differentially expressed genes between early and mature ovarian stages in the female mantis shrimp (Harpiosquilla raphidea) using RNA-Seq DOI Creative Commons
Rachanimuk Hiransuchalert,

Chompoonuch Poarsa,

Patchari Yocawibun

et al.

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 34, P. 101910 - 101910

Published: Jan. 8, 2024

Harpiosquilla raphidea, the largest of mantis shrimps, is a commercially important crustacean species widely distributed in many countries Pacific Ocean. However, no data are currently available regarding molecular mechanisms that regulate reproduction this species. To address knowledge gap, we performed transcriptome sequencing (RNA-Seq) previtellogenic and vitellogenic ovaries female H. compared expression patterns transcripts from two resulting libraries to identify genes involved ovarian development. A total 418,635,748 clean reads were retrieved after removing adapter sequences filtering out low-quality data. The assembled into 242,861 unigenes with an average length 585.27 base pairs (bp) N50 829 bp. search all against NR (non-redundant protein), SwissProt, KEGG, GO, COG databases yielded 53,111; 25,460; 27,255; 26,793; 3838 unigene matches, respectively). Of 53,111 identified database, 30,441 could be functionally annotated, 59.49% which significantly matched L. vannamei. 13,867 full-length 11,441 partial retrieved. In addition, using all-unigenes as reference, 12,765 simple sequence repeats (SSRs) identified. DESeq2 analysis 962 differentially expressed (DEGs) between ovaries, 456 upregulated 506 downregulated. Five validated by quantitative real-time PCR (qPCR). results both RNA-Seq qPCR revealed raphidea vitellogenin gene (HrVtg) was ovaries. open reading frame HrVtg found 7455 bp long, corresponding polypeptide 2485 amino acids. functional pathways here provide valuable dataset for understanding controlling development raphidea.

Language: Английский

Citations

0
Comparative transcriptome analysis of low- and high-latitude populations of Charybdis japonica under temperature stress DOI Creative Commons
Shaolei Sun, Zhiqi He,

Feijun Zhang

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: Nov. 4, 2024

Global climate change has caused rapid temperature changes in marine environments. Understanding how organisms respond to can help predict their richness of future biodiversity. In this study, we examined the gene expression levels and difference pathways that are responsive acute stress low- high-latitude populations shore swimming crab, Charybdis japonica . The two C. were exposed high-temperature stresses (15°C 28°C) used for transcriptome sequencing. Genetic regulatory ability compared determine diverse response crab change. functional enrichment analysis showed low-latitude regulated more genes (938) mainly enriched DNA replication metabolic pathways, whereas less (309) genetic information processing at low-temperature stress. Furthermore, (33) processing, (280) signal transduction cellular process These results implied population was resilient stress, while This study enhances our understanding different geographic varying environments living zone, which could be helpful predicting biodiversity trends intertidal crustaceans under global

Language: Английский

Citations

0
Transcriptomic modifications induced by short-term temperature exposure reveal jellyfish adaptive energetic responses DOI
Edgar Gamero‐Mora, C. A. Nevarez-Lopez, Raúl Llera‐Herrera

et al.

Hydrobiologia, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Language: Английский

Citations

0
Identification and construction of lncRNA-miRNA-mRNA ceRNA networks associated with temperature changes in Sebastiscus marmoratus DOI
Zhujun Li,

Chenyan Shou,

Shaolei Sun

et al.

Journal of Oceanology and Limnology, Journal Year: 2024, Volume and Issue: unknown

Published: July 6, 2024

Language: Английский

Citations

0