Neuromodulation and the toolkit for behavioural evolution: can ecdysis shed light on an old problem? DOI Creative Commons
Luis F. Sullivan,

Matthew S. Barker,

Princess C. Felix

et al.

FEBS Journal, Journal Year: 2022, Volume and Issue: 291(6), P. 1049 - 1079

Published: Oct. 12, 2022

The geneticist Thomas Dobzhansky famously declared: 'Nothing in biology makes sense except the light of evolution'. A key evolutionary adaptation Metazoa is directed movement, which has been elaborated into a spectacularly varied number behaviours animal clades. mechanisms by have evolved, however, remain unresolved. This due, part, to indirect control behaviour genome, provides components for both building and operating brain circuits that generate behaviour. These are adapted respond flexibly environmental contingencies physiological needs can change as function experience. resulting plasticity behavioural expression it difficult characterize homologous elements track their evolution. Here, we evaluate progress identifying genetic substrates evolution suggest examining adaptive changes neuromodulatory signalling may be particularly productive focus future studies. We propose sequences used ecdysozoans moult an attractive model studying role neuromodulation

Language: Английский

Dietary arachidonic acid supplementation promoted cholesterol utilization, lipid deposition and molting for Scylla paramamosain DOI

Shichao Xie,

Yao Deng,

Zheng Tang

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 593, P. 741274 - 741274

Published: June 27, 2024

Language: Английский

Citations

5
Key Neuropeptides Regulating Molting in Pacific White Shrimp (Penaeus vannamei): Insights from Transcriptomic Analysis DOI Creative Commons
Xianliang Li,

Yunjiao Li,

Zecheng Li

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 540 - 540

Published: Feb. 13, 2025

Molting is a vital physiological process essential for the growth and development of Penaeus vannamei, with significant implications aquaculture productivity. This study aimed to identify neuropeptide-related genes involved in molting through transcriptomic analysis. RNA sequencing pre-molt post-molt samples revealed 1203 differentially expressed (DEGs). Functional enrichment analysis indicated that these play roles cuticle formation regulation. Among DEGs, 243 were predicted be neuropeptides based on presence signal peptides absence transmembrane domains. Five key neuropeptide genes-PvCHH, PvMIH, PvEH I, PvCDA II-were identified as critical regulators molting. Their role was further validated RT-qPCR analysis, confirming their close association process. These highlighted this pivotal factors driving P. vannamei. The research are anticipated offer valuable insights into regulation Additionally, synthetic products hold promise improving consistency shrimp aquaculture.

Language: Английский

Citations

0
Crustacean endocrinology DOI
Donald L. Mykles, Lisa Musgrove, Tomer Ventura

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Citations

0
Antidepressants – The new endocrine disruptors? The case of crustaceans DOI
Thomas Knigge

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 583, P. 112155 - 112155

Published: Jan. 6, 2024

Language: Английский

Citations

3
Corazonin Stimulates Ecdysteroid Synthesis during the Molting Process of the Swimming Crab, Portunus trituberculatus DOI Creative Commons
Xi Xie, Jun Zhang,

Shisheng Tu

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(8), P. 630 - 630

Published: Aug. 18, 2024

The neuropeptide corazonin (Crz) exerts diverse physiological effects in insects, yet its role crustaceans remains elusive. abundant expression of Crz receptor (CrzR) the Y-organs several suggests a potential involvement regulating ecdysteroid synthesis. In this study, we examined PtCrz on synthesis during molting period Portunus trituberculatus through treatments and PtCrzR silencing. Our results showed that peptide stimulates levels gene involved ecdysteroidogenesis both vitro vivo, whereas dsPtCrzR had opposite associated expression. Thus, our study may modulate via Y-organ-expressed PtCrzR. Furthermore, also discovered PtCrz/PtCrzR signaling PtETH Notably, inhibitory effect or can be reversed by treatment, suggesting existence multiple receptors for PtCrz. This provides new insights into function crustacean and, first time, elucidates presence stimulate crustaceans.

Language: Английский

Citations

1
Characterization and Analysis of the Functional Differences of the Two Eclosion Hormones in Regulating Molting in the White Shrimp Litopenaeus vannamei DOI Open Access

Yunjiao Li,

Zecheng Li,

Hongmei Ran

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12813 - 12813

Published: Nov. 28, 2024

Litopenaeus vannamei, with an annual production of 5–6 million tons and a value USD 50–60 billion, is cornerstone global aquaculture. However, molting-related losses 5–20% significantly impact this industry, the physiological mechanisms molting remain unclear. This study aims to elucidate role eclosion hormone (EH) in regulation enhances understanding physiology L. vannamei. investigated vannamei regulation. Two EH cDNAs, LvEH I II, were identified, their expression patterns across tissues seven stages (A, B, C, D0, D1, D2, D3) analyzed. was predominantly expressed gill, epidermis, eyestalk, while II mainly eyestalk brain. highly gills at D2 D3 molting, whereas both (brain) (eyestalk) stages. RNA interference (RNAi) targeting revealed its critical as silencing disrupted molting-regulation genes, ETH, CCAP, CHH, CDA, bursicon (Burs), delaying process. These findings highlight indispensable for normal provide foundation developing effective management strategies reduce industry losses.

Language: Английский

Citations

1
Transcriptome responses of RNAi-mediated ETH knockdown in Scylla paramamosain at different premolt substages DOI Creative Commons

Siuming-Francis Chan,

Qi-Qiao Wen,

Chun-Mei Ao

et al.

Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13

Published: July 28, 2022

Ecdysis triggering hormone (ETH) plays an important role in molting, reproduction, and courtship behavior insects. To investigate the potential downstream pathways genes of ETH Scylla paramamosain , RNA interference (RNAi) was conducted on crabs at early (D0) late (D2) premolt substages, transcriptome profiles each group were compared by sequencing. Real-time quantitative polymerase chain reaction (RT-qPCR) semiquantitative (RT-PCR) results showed a significant knockdown D0 stage, whereas increase shown conversely D2 substage after injection dsETH . A total 242,979 transcripts assembled, 44,012 unigenes identified. Transcriptomic comparison between substages 2,683 differentially expressed (DEGs); these enriched ribosome related to transcription factor complex cell part. Twenty DEGs identified -injected dsGFP substage; involved carbohydrate metabolism, one carbon pool folate, chitin binding. Twenty-six calcium channel inhibitor activity, fat digestion absorption, cardiac muscle contraction. RT-qPCR verified differential expression selected genes. In conclusion, are more active preparing macromolecular that is needed for molting. Moreover, has roles binding substage, while turns be contraction facilitate occurrence The provide valuable insight into regulation crustacean

Language: Английский

Citations

7
Unravelling the neuropeptidome of the ornate spiny lobster Panulirus ornatus: A focus on peptide hormones and their processing enzymes expressed in the reproductive tissues DOI
Susan Glendinning, Quinn P. Fitzgibbon, Gregory Smith

et al.

General and Comparative Endocrinology, Journal Year: 2022, Volume and Issue: 332, P. 114183 - 114183

Published: Nov. 28, 2022

Language: Английский

Citations

5
Identification of ETH receptor and its possible roles in the mud crab Scylla paramamosain DOI

Yiwei Tang,

Fang Liu, Lü Li

et al.

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology, Journal Year: 2024, Volume and Issue: 297, P. 111692 - 111692

Published: July 6, 2024

Language: Английский

Citations

0
Network toxicology focused investigation on the impacts of inorganic arsenic and cadmium on human and ecosystem health DOI Open Access
Nikhil Chivukula, Shreyes Rajan Madgaonkar, K.H. Ramesh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Abstract Heavy metals like arsenic and cadmium are persistent environmental pollutants that pose serious health risks to humans ecosystems due their toxicity, bioaccumulation potential, frequent presence in consumer products. Network toxicology offers a holistic silico framework elucidate the complex biological mechanisms of thereby supporting New Approach Methodologies (NAMs) for toxicity assessment. In this study, network toxicological tools were utilized investigate arsenic- cadmium-induced toxicities. Toxicity endpoints associated with inorganic compounds curated from six exposome-relevant databases mapped key events (KEs) across adverse outcome pathways (AOPs) cataloged AOP-Wiki. This led construction stressor-AOP networks, revealing 51 AOPs 78 cadmium, facilitated mechanistic case studies relevant human ecological health. concentrations bioconcentration factors ECOTOX database then used construct stressor-species helped identify species vulnerable potentially bioaccumalate these chemicals. Further, sensitivity distributions (SSDs) toxicity-normalized SSDs (SSDn), provided comparative prioritizing risk assessments. integrating SSD data networks identified groups particularly sensitive exposure, enhancing networks’ utility The related generated study freely available further research at https://cb.imsc.res.in/heavymetaltox/ . Overall, comprehensive perspective on impact compounds, One Health approach regulatory mitigation strategies.

Language: Английский

Citations

0