Bacterial inoculation manipulates the coral epigenome DOI Creative Commons
Adam R. Barno, Helena D. M. Villela, Pedro Cardoso

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 27, 2024

Summary Environmental shifts can elicit epigenetic modifications in corals, which regulate gene expression and physiology, though it remains unclear if associated bacteria also induce such changes. Here, we inoculated nubbins of the coral Pocillopora verrucosa with an opportunistic pathogen, Vibrio coralliilyticus , and/or a probiotic, Cobetia sp., subjected to heat stress. We discovered that epigenomes responded already after 19 days stress 28 bacterial inoculation. Pathogen led DNA methylation changes not seen other treatments. Notably, probiotic was able mitigate specific glutamate receptor pathway, this correlated increased resilience higher survival rates corals. Importantly, marks persisted even temperature removed, suggesting epigenome acclimation. Thus, bacterial-induced may instigate long-term host resilience.

Language: Английский

Probiotics reshape the coral microbiome in situ without detectable off-target effects in the surrounding environment DOI Creative Commons
Nathalia Delgadillo-Ordoñez, Neus Garcias‐Bonet, Inês Raimundo

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: April 9, 2024

Beneficial microorganisms for corals (BMCs), or probiotics, can enhance coral resilience against stressors in laboratory trials. However, the ability of probiotics to restructure microbiome situ is yet be determined. As a first step elucidate this, we inoculated putative probiotic bacteria (pBMCs) on healthy colonies Pocillopora verrucosa Red Sea, three times per week, during 3 months. pBMCs significantly influenced microbiome, while surrounding seawater and sediment remained unchanged. The genera Halomonas, Pseudoalteromonas, Bacillus were enriched probiotic-treated corals. Furthermore, treatment also correlated with an increase other beneficial groups (e.g., Ruegeria Limosilactobacillus), decrease potential pathogens, such as Vibrio. all (treated non-treated) throughout experiment, could not track health improvements protection stress. Our data indicate that healthy, therefore stable, microbiomes restructured situ, although repeated continuous inoculations may required these cases. Further, our study provides supporting evidence that, at studied scale, have no detectable off-target effects near

Language: Английский

Citations

19
Microbial-Based Therapies to Restore and Rehabilitate Disrupted Coral Health DOI
Melanie Dörr, Adam R. Barno, Helena D. M. Villela

et al.

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 181 - 195

Published: Jan. 1, 2025

Language: Английский

Citations

1
Unlocking the genomic potential of Red Sea coral probiotics DOI Creative Commons
Inês Raimundo,

Phillipe M. Rosado,

Adam R. Barno

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 24, 2024

Abstract The application of beneficial microorganisms for corals (BMC) decreases the bleaching susceptibility and mortality rate corals. BMC selection is typically performed via molecular biochemical assays, followed by genomic screening traits. Herein, we present a comprehensive in silico framework to explore set six putative strains. We extracted high-quality DNA from coral samples collected Red Sea PacBio sequencing. identified traits mechanisms associated with each strain as well proposed new mechanisms, such chemotaxis presence phages bioactive secondary metabolites. prophages two studied strains suggests their possible distribution within bacteria. also detected various metabolites, terpenes, ectoines, lanthipeptides, lasso peptides. These metabolites possess antimicrobial, antifungal, antiviral, anti-inflammatory, antioxidant activities play key roles health reducing effects heat stress, high salinity, reactive oxygen species, radiation. Corals are currently facing unprecedented challenges, our revised can help select more efficient use studies on microbiome rehabilitation, resilience, restoration.

Language: Английский

Citations

6
Localization and symbiotic status of probiotics in the coral holobiont DOI Creative Commons
Pedro Cardoso,

L. Hill,

Helena D. M. Villela

et al.

mSystems, Journal Year: 2024, Volume and Issue: 9(5)

Published: April 12, 2024

ABSTRACT Corals establish symbiotic relationships with microorganisms, especially endosymbiotic photosynthetic algae. Although other microbes have been commonly detected in coral tissues, their identity and beneficial functions for host are unclear. Here, we confirm the outcomes of inoculation bacteria selected as probiotics use fluorescence situ hybridization (FISH) to define localization Pocillopora damicornis . Our results show first evidence inherent presence Halomonas sp. Cobetia native even before inoculation. Furthermore, relative enrichment these tissue-associated through corals correlates health improvements, such increases potential, productivity. study suggests status by indicating within gastrodermis epidermis correlating increased abundance active holobiont. This knowledge is crucial facilitate screening application that may not be transient members microbiome. IMPORTANCE Despite promising associated some scarce regarding bacterial cells found tissue, correlation between two aspects still missing. gap limits our understanding actual diversity coral-associated whether symbionts beneficial. Some researchers, example, suggesting probiotic should only focus on very few known bacteria, Endozoicomonas sp., assuming currently tested tissue-associated. provide specific FISH probes expand probiotics. The microorganisms (BMCs) inside tissues gastric cavities also supports notion direct interactions underpin role. a new breakthrough; argue against possibility positive effects BMCs due factors related interaction, simply feeds inoculated or change water quality.

Language: Английский

Citations

4
Building Coral Reef Resilience Through Assisted Restoration DOI
Raquel S. Peixoto, Christian R. Voolstra, Sebastian Staab

et al.

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 235 - 243

Published: Jan. 1, 2025

Language: Английский

Citations

0
Phototrophic bacteria as potential probiotics for corals DOI Creative Commons
Eslam O. Osman, Neus Garcías-Bonet, Pedro Cardoso

et al.

npj Biodiversity, Journal Year: 2025, Volume and Issue: 4(1)

Published: April 29, 2025

Coral-associated microorganisms provide crucial nutritional, protective, and developmental benefits, yet many functional traits remain unexplored. Phototrophic bacteria may enhance coral nutrition reduce oxidative stress during bleaching via photosynthesis antioxidant production. Despite this potential, their role in the holobiont's energy budget heat resilience is understudied. This review explores potential of phototrophic to health under environmental stress.

Language: Английский

Citations

0
Validating the use of ROS-scavenging bacteria as probiotics to increase coral resilience to thermal stress DOI
Xiaoyu Tang, Qingsong Yang, Ying Zhang

et al.

Journal of Oceanology and Limnology, Journal Year: 2024, Volume and Issue: 42(4), P. 1242 - 1260

Published: June 15, 2024

Language: Английский

Citations

1
Microscale sampling of the coral gastrovascular cavity reveals a gut-like microbial community DOI Creative Commons
Elena Bollati, David Hughes, David J. Suggett

et al.

Animal Microbiome, Journal Year: 2024, Volume and Issue: 6(1)

Published: Oct. 8, 2024

Animal guts contain numerous microbes, which are critical for nutrient assimilation and pathogen defence. While corals other Cnidaria lack a true differentiated gut, they possess semi-enclosed gastrovascular cavities (GVCs), where vital processes such as digestion, reproduction symbiotic exchanges take place. The microbiome harboured in GVCs is therefore likely key to holobiont fitness, but remains severely understudied due challenges of working these small compartments. Here, we developed minimally invasive methodologies sample the GVC coral polyps characterise microbial communities within. We used glass capillaries, low dead volume microneedles, or nylon microswabs individual from six species corals, then applied low-input DNA extraction microliter samples. Microsensor measurements revealed anoxic hypoxic micro-niches, persist even under prolonged illumination with saturating irradiance. These niches enriched putatively microaerophilic facultatively anaerobic taxa, Epsilonproteobacteria. Some core taxa found Lobophyllia hemprichii Great Barrier Reef were also detected conspecific colonies held aquaria, indicating that associations unlikely be transient. Our findings suggest chemically microbiologically similar gut higher Metazoa. Given importance microbiomes mediating animal health, harnessing "gut microbiome" may foster novel active interventions aimed at increasing resilience reefs climate crisis.

Language: Английский

Citations

1
Probiotics reshape the coral microbiome in situ without affecting the surrounding environment DOI Creative Commons
Nathalia Delgadillo-Ordoñez, Neus Garcias‐Bonet, Inês Raimundo

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 31, 2023

Abstract Beneficial Microorganisms for Corals (BMCs), or probiotics, enhance coral resilience against stressors in laboratory trials, being the only sustainable treatment currently explored to retain threatened native corals. However, ability of probiotics restructure microbiome situ is yet be determined. To elucidate this, we inoculated BMCs on Pocillopora verrucosa colonies Red Sea three months. significantly influenced microbiome, while those surrounding seawater and sediment appeared unchanged. The genera Halomonas Pseudoalteromonas were enriched probiotic-treated Furthermore, probiotic correlated with an increase beneficial groups (e.g., Ruegeria Limosilactobacillus ), potential pathogens, like Vibrio , decreased abundance. Notably, treated non-treated corals remained healthy throughout experiment. Our data indicate feasibility using real-world conservation efforts through restructuring without off-target changes environment.

Language: Английский

Citations

3
Unlocking the genomic potential of Red Sea coral probiotics DOI Creative Commons
Inês Raimundo,

Phillipe M. Rosado,

Adam R. Barno

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 28, 2024

Abstract The application of beneficial microorganisms for corals (BMC), both in vivo and situ, decreases the bleaching susceptibility mortality rate corals. BMC selection is typically performed via molecular biochemical assays, followed by genomic screening traits. Herein, we present an improved updated silico framework a set six putative strains. We extracted high-quality DNA from coral samples collected Red Sea PacBio sequencing. identified traits mechanisms associated with each strain as well proposed new mechanisms, such chemotaxis presence phages bioactive secondary metabolites. prophages four studied strains suggests their widespread distribution within bacteria. This newly indicates importance BMCs they can expand bacterial metabolic potential offer competitive advantage against pathogens. also detected various metabolites, terpenes, ectoines, lanthipeptides, lasso peptides. These metabolites possess antimicrobial, antifungal, antiviral, anti-inflammatory, antioxidant activities play key roles health reducing effects heat stress, high salinity, reactive oxygen species, radiation. Corals are currently facing unprecedented challenges, our revised help select more efficient use studies on microbiome rehabilitation, resilience, restoration.

Language: Английский

Citations

0