Unlocking the genomic potential of Red Sea coral probiotics DOI Creative Commons
Inês Raimundo,

Phillipe M. Rosado,

Adam R. Barno

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 28, 2024

Abstract The application of beneficial microorganisms for corals (BMC), both in vivo and situ, decreases the bleaching susceptibility mortality rate corals. BMC selection is typically performed via molecular biochemical assays, followed by genomic screening traits. Herein, we present an improved updated silico framework a set six putative strains. We extracted high-quality DNA from coral samples collected Red Sea PacBio sequencing. identified traits mechanisms associated with each strain as well proposed new mechanisms, such chemotaxis presence phages bioactive secondary metabolites. prophages four studied strains suggests their widespread distribution within bacteria. This newly indicates importance BMCs they can expand bacterial metabolic potential offer competitive advantage against pathogens. also detected various metabolites, terpenes, ectoines, lanthipeptides, lasso peptides. These metabolites possess antimicrobial, antifungal, antiviral, anti-inflammatory, antioxidant activities play key roles health reducing effects heat stress, high salinity, reactive oxygen species, radiation. Corals are currently facing unprecedented challenges, our revised help select more efficient use studies on microbiome rehabilitation, resilience, restoration.

Language: Английский

The coral microbiome in sickness, in health and in a changing world DOI
Christian R. Voolstra, Jean‐Baptiste Raina, Melanie Dörr

et al.

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(8), P. 460 - 475

Published: March 4, 2024

Language: Английский

Citations

45
The Fourth Global Coral Bleaching Event: Where do we go from here? DOI Creative Commons
James Davis Reimer, Raquel S. Peixoto, Sarah W. Davies

et al.

Coral Reefs, Journal Year: 2024, Volume and Issue: 43(4), P. 1121 - 1125

Published: May 30, 2024

The Fourth Global Coral Bleaching Event was officially confirmed by the National Oceanic and Atmospheric Administration (NOAA) International Reef Initiative (ICRI) on April 15, 2024, with press releases a coordinated call

Language: Английский

Citations

33
Probiotics reshape the coral microbiome in situ without detectable off-target effects in the surrounding environment DOI Creative Commons
Nathalia Delgadillo-Ordoñez, Neus Garcias‐Bonet, Inês Raimundo

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: April 9, 2024

Beneficial microorganisms for corals (BMCs), or probiotics, can enhance coral resilience against stressors in laboratory trials. However, the ability of probiotics to restructure microbiome situ is yet be determined. As a first step elucidate this, we inoculated putative probiotic bacteria (pBMCs) on healthy colonies Pocillopora verrucosa Red Sea, three times per week, during 3 months. pBMCs significantly influenced microbiome, while surrounding seawater and sediment remained unchanged. The genera Halomonas, Pseudoalteromonas, Bacillus were enriched probiotic-treated corals. Furthermore, treatment also correlated with an increase other beneficial groups (e.g., Ruegeria Limosilactobacillus), decrease potential pathogens, such as Vibrio. all (treated non-treated) throughout experiment, could not track health improvements protection stress. Our data indicate that healthy, therefore stable, microbiomes restructured situ, although repeated continuous inoculations may required these cases. Further, our study provides supporting evidence that, at studied scale, have no detectable off-target effects near

Language: Английский

Citations

19
Horizon scanning the application of probiotics for wildlife DOI Creative Commons
Neus Garcias‐Bonet, Anna Roik, Braden Tierney

et al.

Trends in Microbiology, Journal Year: 2023, Volume and Issue: 32(3), P. 252 - 269

Published: Sept. 25, 2023

The provision of probiotics benefits the health a wide range organisms, from humans to animals and plants. Probiotics can enhance stress resilience endangered many which are critically threatened by anthropogenic impacts. use so-called 'probiotics for wildlife' is nascent application, field needs reflect on standards its development, testing, validation, risk assessment, deployment. Here, we identify main challenges this emerging intervention provide roadmap validate effectiveness wildlife probiotics. We cover essential inert negative controls in trials investigation probiotic mechanisms action. also suggest alternative microbial therapies that could be tested parallel with application. Our recommendations align approaches used humans, aquaculture, plants concept wildlife.

Language: Английский

Citations

36
Microbial-Based Therapies to Restore and Rehabilitate Disrupted Coral Health DOI
Melanie Dörr, Adam R. Barno, Helena D. M. Villela

et al.

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 181 - 195

Published: Jan. 1, 2025

Language: Английский

Citations

1
Disparate genetic divergence patterns in three corals across a pan-Pacific environmental gradient highlight species-specific adaptation DOI Creative Commons
Christian R. Voolstra, Benjamin C. C. Hume, Eric Armstrong

et al.

npj Biodiversity, Journal Year: 2023, Volume and Issue: 2(1)

Published: July 7, 2023

Tropical coral reefs are among the most affected ecosystems by climate change and face increasing loss in coming decades. Effective conservation strategies that maximize ecosystem resilience must be informed accurate characterization of extant genetic diversity population structure together with an understanding adaptive potential keystone species. Here we analyzed samples from Tara Pacific Expedition (2016-2018) completed 18,000 km longitudinal transect Ocean sampling three widespread corals-Pocillopora meandrina, Porites lobata, Millepora cf. platyphylla-across 33 sites 11 islands. Using deep metagenomic sequencing 269 colonies conjunction morphological analyses variability data, can show despite a targeted encompasses multiple cryptic These species exhibit disparate biogeographic patterns and, importantly, distinct evolutionary identical environmental regimes. Our findings demonstrate on basin scale trajectories species-specific only part predicted environment. This highlights integrate multi-species investigations to discern genomic footprints shaped selection as well for change.

Language: Английский

Citations

22
Roadmap for the integration of environmental microbiomes in risk assessments under EFSA's remit DOI Open Access
Frédéric Debode,

Simon Caulier,

Sébastien Demeter

et al.

EFSA Supporting Publications, Journal Year: 2024, Volume and Issue: 21(2)

Published: Feb. 1, 2024

Scientific interest in the use of environmental microbiomes for risk assessment is rapidly growing, as exemplified by various EFSA opinions. In absence official regulatory guidelines on how to integrate assessment, aims this report are therefore determine whether microbiome studies can be used such purposes, and propose a roadmap integration assessments under EFSA's remit. The identifies current gaps (in terms knowledge from technical point view) barriers that might delay implementation methods, offers recommendations standardised (multi-)omics techniques purposes. Our main findings identified five priorities: (i) defining core (what it encompasses what made of, including identification bioindicators) assess impact any type disturbance; (ii) standardising methodologies protocols, sampling interpretation, guarantee comparability analyses; (iii) developing tools facilitate interpretation; (iv) collecting microbiome-based data shared, curated maintained databases; (v) setting up European Network Microbiome Laboratories reach an agreement standardise studies, interactions between researchers access or samples, actively include multiple stakeholders discussions involving assessment. There both short- longer-term priorities, all which highlight need mobilise concurrently different agencies institutions, well research. also points out capacity building training, acceptance emerging technology, communication issues. These will hopefully contribute elaboration widely accepted framework dealing with.

Language: Английский

Citations

8
Unlocking the genomic potential of Red Sea coral probiotics DOI Creative Commons
Inês Raimundo,

Phillipe M. Rosado,

Adam R. Barno

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 24, 2024

Abstract The application of beneficial microorganisms for corals (BMC) decreases the bleaching susceptibility and mortality rate corals. BMC selection is typically performed via molecular biochemical assays, followed by genomic screening traits. Herein, we present a comprehensive in silico framework to explore set six putative strains. We extracted high-quality DNA from coral samples collected Red Sea PacBio sequencing. identified traits mechanisms associated with each strain as well proposed new mechanisms, such chemotaxis presence phages bioactive secondary metabolites. prophages two studied strains suggests their possible distribution within bacteria. also detected various metabolites, terpenes, ectoines, lanthipeptides, lasso peptides. These metabolites possess antimicrobial, antifungal, antiviral, anti-inflammatory, antioxidant activities play key roles health reducing effects heat stress, high salinity, reactive oxygen species, radiation. Corals are currently facing unprecedented challenges, our revised can help select more efficient use studies on microbiome rehabilitation, resilience, restoration.

Language: Английский

Citations

6
Probiotics mitigate thermal stress- and pathogen-driven impacts on coral skeleton DOI Creative Commons
Mahdi Moradi,

Phillipe R. Magalhaes,

Raquel S. Peixoto

et al.

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 10

Published: Sept. 19, 2023

Threats leading to a reduction in coral populations are apparent worldwide. Several different approaches have been tested accelerate the adaptation of corals changing climate. Here, we evaluated skeleton structure, crystal habit, and chemical changes Pocillopora damicornis response pathogen ( Vibrio coralliilyticus ) probiotic (Beneficial Microorganisms for Corals, BMCs) inoculation under ambient conditions (26 °C) thermal stress (30 during 50-day mesocosm experiment. The skeletons were analyzed using microtomography, energy-dispersive x-ray spectroscopy (EDX/SEM), densitometry investigate skeleto-physico-chemical micro-morphological porosity, median pore-size diameter, Mg/Ca, Sr/Ca, mineral density (g/cm 2 content (g –2 ). results indicate considerable caused by both temperature microbial inoculation. Most importantly, lower (to ∼ x̄ 0.5 g/cm higher porosity (up 47%) correlated with V. mitigated probiotics. BMCs also substantially increased calcification, as evidenced Mg/Ca thermally stressed corals. At micron scale, aragonite fibbers precipitated experiments showed an acicular habit pathogen-inoculated kept at 30 °C. In contrast, spherulitic characteristic high growth rates, was observed inoculated . Our findings reveal that had notable impacts on properties, including density, morphology, short period time, which highlights potential shifts climate warming environmental quality. Interestingly, played role maintaining properties calcification.

Language: Английский

Citations

12
Microbial solutions must be deployed against climate catastrophe DOI Creative Commons
Raquel S. Peixoto, Christian R. Voolstra, Lisa Y. Stein

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 11, 2024

This paper is a call to action. By publishing concurrently across journals like an emergency bulletin, we are not merely making plea for awareness about climate change. Instead, demanding immediate, tangible steps that harness the power of microbiology and expertise researchers policymakers safeguard planet future generations.

Language: Английский

Citations

4