Mesenchymal Stem Cells Lose the Senescent Phenotype Under 3D Cultivation DOI Creative Commons
Olga Krasnova, Anastasiia Kovaleva,

Arina Saveleva

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 9, 2023

Abstract Background Three-dimensional (3D) cell culture is widely used in various fields of biology. In comparison to conventional two-dimensional (2D) culture, 3D facilitates a more accurate replication thein vivomicroenvironment, which essential for obtaining relevant results. The application techniques regenerative medicine, particularly mesenchymal stem (MSC)-based research, has been extensively studied. Many these studies focus on the enhanced paracrine activity MSCs cultured environments. However, few cellular processes that occur during cultivation. Methods this study, we studied changes occurring within 3D-cultured (3D-MSCs). Specifically, examined expression numerous senescent-associated markers, actin cytoskeleton structure, architecture Golgi apparatus and localization mTOR, one main positive regulators replicative senescence. addition, assessed whether selective elimination senescent cells occurs upon culturing by using sorting based autofluorescence. Results Our findings indicate 3D-MSCs were able overcome We discovered revealed cultivation leads nuclear resulting decrease its active cytoplasmic form. Furthermore, confirmed rejuvenation rather than their from bulk population. Conclusion These novel insights into biology can be applied research medicine senescence MSC heterogeneity as they often pose significant concerns regarding safety effectiveness therapeutic purposes.

Language: Английский

The Hidden Power of the Secretome: Therapeutic Potential on Wound Healing and Cell-Free Regenerative Medicine—A Systematic Review DOI Open Access
Jhon W. Prado-Yupanqui, Lourdes Ramírez-Orrego, Denny Cortez

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1926 - 1926

Published: Feb. 23, 2025

Various types of wounds represent a persistent healthcare burden that demands innovative and effective therapeutic solutions. Innovative approaches have emerged focus on skin regeneration with minimal side effects. One such method is cell-free therapy utilizes the secretome human mesenchymal stem cells (hMSCs) as promising alternative to traditional cell-based therapies, leveraging complex mixture bioactive molecules, including growth factors, cytokines, extracellular vesicles, accelerate tissue regeneration. This systematic review synthesizes findings 35 studies evaluating impact hMSC-derived secretomes wound healing, their regenerative, immunomodulatory, angiogenic The influence MSC sources (adipose tissue, bone marrow, umbilical cord) culture conditions composition efficacy in cutaneous healing process examined, highlighting potential regenerative medicine. also explores emerging preclinical clinical applications, results, enhanced fibroblast proliferation, reduced inflammation, improved matrix remodeling. In addition, advances secretome-based biomaterials, hydrogels scaffolds, which optimize delivery are discussed. Despite growing body evidence supporting safety secretomes, challenges remain regarding standardization, large-scale production, validation. highlights MSC-derived next-generation approach for

Language: Английский

Citations

0
Effects of Hydrogels on Mesenchymal Stem/Stromal Cells Paracrine Activity and Extracellular Vesicles Production DOI Creative Commons

Oscar Fabian Garcia‐Aponte,

Simon Kahlenberg,

Dimitrios Kouroupis

et al.

Journal of Extracellular Vesicles, Journal Year: 2025, Volume and Issue: 14(3)

Published: March 1, 2025

ABSTRACT Mesenchymal stem/stromal cells (MSCs) are a valuable source of paracrine factors, as they have remarkable secretory capacity, and there is sizeable knowledge base to develop industrial clinical production protocols. Promising cell‐free approaches for tissue regeneration immunomodulation driving research towards secretome applications, among which extracellular vesicles (EVs) steadily gaining attention. However, the manufacturing application EVs limited by insufficient yields, gaps, low standardization. Facing these limitations, hydrogels represent versatile three‐dimensional (3D) culture platform that can incorporate matrix (ECM) components mimic natural stem cell environment in vitro; via niche‐mimicking properties, regulate MSCs’ morphology, adhesion, proliferation, differentiation secretion capacities. impact hydrogel's architectural, biochemical biomechanical properties on remains poorly understood, field still its infancy interdependency parameters compromises comparability studies. Therefore, this review summarizes discusses reported effects hydrogel encapsulation MSC‐EVs. Considering cell‐material interactions overall activity MSCs, we identify persistent challenges from standardization process control, outline future paths research, such synergic use bioreactors enhance MSC‐EV generation.

Language: Английский

Citations

0
Mesenchymal stem cells lose the senescent phenotype under 3D cultivation DOI Creative Commons
Olga Krasnova, Anastasia Kovaleva,

А. А. Савельева

et al.

Stem Cell Research & Therapy, Journal Year: 2023, Volume and Issue: 14(1)

Published: Dec. 18, 2023

Abstract Background Three-dimensional (3D) cell culture is widely used in various fields of biology. In comparison to conventional two-dimensional (2D) culture, 3D facilitates a more accurate replication the vivo microenvironment, which essential for obtaining relevant results. The application techniques regenerative medicine, particularly mesenchymal stem (MSC)-based research, has been extensively studied. Many these studies focus on enhanced paracrine activity MSCs cultured environments. However, few cellular processes that occur during cultivation. Methods this work, we studied changes occurring within 3D-cultured (3D-MSCs). Specifically, examined expression numerous senescent-associated markers, actin cytoskeleton structure, architecture Golgi apparatus and localization mTOR, one main positive regulators replicative senescence. addition, assessed whether selective elimination senescent cells occurs upon culturing by using sorting based autofluorescence. Results Our findings indicate 3D-MSCs were able lose senescence markers under conditions. We observed revealed cultivation leads nuclear resulting decrease its active cytoplasmic form. Additionally, our provide evidence promotes phenotypic reversion phenotype rather than their removal from bulk population. Conclusion These novel insights into biology can be applied research medicine overcome MSC heterogeneity as they often pose significant concerns regarding safety effectiveness therapeutic purposes.

Language: Английский

Citations

7
Mesenchymal Stem Cells Lose the Senescent Phenotype Under 3D Cultivation DOI Creative Commons
Olga Krasnova, Anastasiia Kovaleva,

Arina Saveleva

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 9, 2023

Abstract Background Three-dimensional (3D) cell culture is widely used in various fields of biology. In comparison to conventional two-dimensional (2D) culture, 3D facilitates a more accurate replication thein vivomicroenvironment, which essential for obtaining relevant results. The application techniques regenerative medicine, particularly mesenchymal stem (MSC)-based research, has been extensively studied. Many these studies focus on the enhanced paracrine activity MSCs cultured environments. However, few cellular processes that occur during cultivation. Methods this study, we studied changes occurring within 3D-cultured (3D-MSCs). Specifically, examined expression numerous senescent-associated markers, actin cytoskeleton structure, architecture Golgi apparatus and localization mTOR, one main positive regulators replicative senescence. addition, assessed whether selective elimination senescent cells occurs upon culturing by using sorting based autofluorescence. Results Our findings indicate 3D-MSCs were able overcome We discovered revealed cultivation leads nuclear resulting decrease its active cytoplasmic form. Furthermore, confirmed rejuvenation rather than their from bulk population. Conclusion These novel insights into biology can be applied research medicine senescence MSC heterogeneity as they often pose significant concerns regarding safety effectiveness therapeutic purposes.

Language: Английский

Citations

0