Disentangling the contributions of initial heterogeneities and dynamic stress adaptation to nonlinearities in bacterial survival curves DOI Creative Commons
Leonidas Georgalis, Kah Yen Claire Yeak,

Christina Tsimpou

et al.

Food Research International, Journal Year: 2023, Volume and Issue: 173, P. 113385 - 113385

Published: Aug. 18, 2023

The deviations from log-linearity that are often observed in bacterial survivor curves can be explained using different arguments, both biological and experimental. In this study, we used Bacillus subtilis as a model organism to demonstrate the generally accepted vitalistic arguments (initial heterogeneities stress resistance of cells population) may fail describe microbial inactivation some situations. sense, showed how dynamic acclimation during an isothermal treatment provides alternative explanation for with upwards curvature. We also provided innovative experimental approach based on preadaptation experiments evaluate which hypothesis is more suitable response. Furthermore, our results define bounds possible take place treatments, concluding magnitude larger treatments than experiments. evaluated contribution SigB general response system heat by comparing survival wt ΔsigB mutant. Both strains survived better 51, 52.5 55 °C when were pre-adapted at 48 non-pre-adapted cells. However, was less resistant due missing system. Although these conclusions B. organism, study first step towards development novel methodology able estimate effects only This would improve models developed within predictive microbiology community, improving ability predict industrial most dynamic.

Language: Английский

RpoS and the bacterial general stress response DOI
Sophie Bouillet, Taran Bauer, Susan Gottesman

et al.

Microbiology and Molecular Biology Reviews, Journal Year: 2024, Volume and Issue: 88(1)

Published: Feb. 27, 2024

SUMMARYThe general stress response (GSR) is a widespread strategy developed by bacteria to adapt and respond their changing environments. The GSR induced one or multiple simultaneous stresses, as well during entry into stationary phase leads global that protects cells against stresses. alternative sigma factor RpoS the central regulator in

Language: Английский

Citations

20
Prediction and validation of novel SigB regulon members in Bacillus subtilis and regulon structure comparison to Bacillales members DOI Creative Commons
Kah Yen Claire Yeak, Jos Boekhorst,

Michiel Wels

et al.

BMC Microbiology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Jan. 18, 2023

Sigma factor B (SigB) is the central regulator of general stress response in Bacillus subtilis and regulates a group genes to various stressors, known as SigB regulon members. Genes that are directly regulated by contain promotor binding motif (PBM) with previously identified consensus sequence.In this study, refined PBMs were derived different spacer compositions lengths (N12-N17) taken into account. These used identify putative SigB-regulated B. genome, revealing 255 genes: 99 had been described literature 156 newly identified, increasing number members (with without PBM) > 500 subtilis. The assigned five categories (I-V) based on their similarity original sequences. functionalities selected representatives per category assessed using promoter-reporter fusions wt ΔsigB mutants upon exposure heat, ethanol, salt stress. activity PrsbV (I) positive control was induced all three stressors. PytoQ (II) showed SigB-dependent only whereas PpucI N17 PylaL (III) N16 mild induction regardless heat/ethanol/salt PywzA PyaaI (IV) displayed ethanol-specific activities despite lower-level conserved - 10 motif. PgtaB (V) SigB-induced under ethanol while lacking region. PygaO PykaA did not show evident changes conditions tested having PBM highly resembled consensus. extended candidates mainly involved coping but also engaged other cellular processes. Orthologs Bacillales genomes, PBM. Additionally, integration signals activate predicted these indicating signaling species-specific.The entire regulatory network sophisticated yet fully understood even for well-characterized organism 168. Knowledge information gained study can be further studies uncover complete picture role species.

Language: Английский

Citations

13
Dissecting cell heterogeneities in bacterial biofilms and their implications for antibiotic tolerance DOI

Mayra C Obando,

Diego O. Serra

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 78, P. 102450 - 102450

Published: Feb. 28, 2024

Language: Английский

Citations

5
Rok from B. subtilis: Bridging genome structure and transcription regulation DOI Creative Commons
Amanda M. Erkelens, Bert van Erp, Wilfried J. J. Meijer

et al.

Molecular Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: March 21, 2024

Abstract Bacterial genomes are folded and organized into compact yet dynamic structures, called nucleoids. Nucleoid orchestration involves many factors at multiple length scales, such as nucleoid‐associated proteins liquid–liquid phase separation, has to be compatible with replication transcription. Possibly, genome organization plays an intrinsic role in transcription regulation, addition classical factors. In this review, we provide arguments supporting view using the Gram‐positive bacterium Bacillus subtilis a model. Proteins BsSMC, HBsu Rok all impact structure of B. chromosome. Particularly for Rok, there is compelling evidence that it combines its structural function global gene regulator. Many studies describe either but rarely both addressed same time. Here, review sides coin integrate them one forms unusually stable DNA–DNA bridges ability likely underlies repressive effect on by preventing RNA polymerase from binding DNA or trapping inside loops. Partner needed change relieve Rok‐mediated repression. Lastly, investigate which features characterize H‐NS‐like proteins, family that, present, lacks clear definition.

Language: Английский

Citations

5
Rapid Aggregation of Staphylococcus aureus in Synovial Fluid Is Influenced by Synovial Fluid Concentration, Viscosity, and Fluid Dynamics, with Evidence of Polymer Bridging DOI
Amelia Staats, Peter W. Burback, Andrew Schwieters

et al.

mBio, Journal Year: 2022, Volume and Issue: 13(2)

Published: March 7, 2022

Early bacterial survival in the postsurgical joint is still a mystery. Recently, synovial fluid-induced aggregation was proposed as potential mechanism of protection upon entry into joint. As fluid secreted back cavity following surgery, rapid fluctuations concentrations, composition, and viscosity occur. These changes, along with movement resulting from postoperative motion, modify environment potentially affect kinetics aggregate formation. Through this work, we sought to evaluate influence exposure time, concentration, viscosity, dynamics on aggregation. Furthermore, aimed elucidate primary formation by assessing interaction adhesins polymer fibrinogen. Following incubation under each simulated condition, aggregates were imaged using confocal microscopy. Our analysis revealed two distinct phenotypes, depending whether conducted static or dynamic conditions. Using surface adhesin mutant, have narrowed down genetic determinants for identified essential host polymers. We report here that influenced various changes specific environment. While now evidence select polymers facilitate bridging through adhesins, suspect their utility limited increasing recovers ability bacteria proteins present high-viscosity conditions, yielding large, globular aggregates. IMPORTANCE Infection major complication knee hip replacement which used treat arthritis damage. shown Staphylococcus aureus, common pathogen, contact fluid. Within seconds, cells interact cell wall adhesins. The these likely aids early joint, contributing likelihood developing an infection. By strengthening our basic understanding mechanics clinically relevant hope expand knowledge how prevent disrupt reduce more successfully infections.

Language: Английский

Citations

19
Staphylococcus aureus membrane vesicles: an evolving story DOI
X. Wang, Jean C. Lee

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 32(11), P. 1096 - 1105

Published: April 26, 2024

Language: Английский

Citations

4
iTRAQ-based quantitative proteomic analysis of herbicide stress in Avena ludoviciana Durieu DOI Creative Commons

Hossein Adim,

Leila Fahmideh, Barat Ali Fakheri

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 2, 2025

Winter wild oat (Avena sterilis subsp. ludoviciana (Durieu) Gillet & Magne) has been considered the most common and troublesome weed in wheat fields of Iran. The widespread continuous use herbicides led to emergence development resistant biotypes A. ludoviciana, making it one important herbicide-resistant weeds within field crops. Considering importance understanding mechanisms underlying resistance identifying key proteins involved response Acetyl-coenzyme A carboxylase (ACCase) Acetolactate synthase (ALS) inhibitor ludoviciana. This study aimed identify herbicide using Isobaric Tags for Relative Absolute Quantification (iTRAQ) technique. In this study, a total 18,313 peptides were identified with ≤ 0.01 FDR, which could be classified into 484 protein groups. Additionally, 138 differentially expressed (DEPs) biotype (R), while 93 DEPs susceptible (S). Gene Ontology (GO) analysis revealed that these mainly consisted related photosynthesis, respiration, amino acid synthesis translation, secondary metabolite biosynthesis, defense proteins, detoxification. Furthermore, enrichment pathway Kyoto Encyclopedia Genes Genomes (KEGG) showed pathways included metabolic pathways, carbohydrate metabolism, metabolites, synthesis, photosynthesis. function indicated some such as cytochrome P450, play direct role Overall, results demonstrated complex its ability increase antioxidant capacity through up-regulated detoxification particularly P450 (Q6YSB4), superoxide dismutase (Q0DRV6) polyamine oxidase (Q7XR46). populations, addition activation enzymatic non-enzymatic systems, other strategies reduced photosynthesis increased transcription translation activity, enhanced lipid regulation cellular processes homeostasis, up-regulation associated signaling ion channels herbicide. Overall findings provide new insights different contribute comprehensive

Language: Английский

Citations

0
Multiwalled Carbon Nanotubes Promoted Biofilm Formation and Rhizosphere Colonization of Bacillus subtilis Tpb55 DOI
Xiaolin Lv,

Shanshan Liu,

Yi Cao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Plant growth-promoting bacteria (PGPB) achieve effective colonization by forming a biofilm on the root surface. However, promoting effects and mechanisms of nanomaterials PGPB formation rhizosphere are rarely studied. This study investigated potential mechanism multiwalled carbon nanotubes (MWCNTs) Bacillus subtilis. 10 100 mg/L MWCNTs increased biomass, extracellular polymeric substance components, live/dead cell ratio, spores in biofilms. induced B. subtilis Tpb55 upregulated gene expressions malL, sacX, tasA-tapA, epsA-O correlated with carbohydrate metabolism formation. first stimulated flagellar motility then formation, thus tobacco rhizosphere. Greenhouse experiments showed that combination reduced occurrence black shank. Therefore, have broad application enhancing effectiveness agricultural disease control yield enhancement.

Language: Английский

Citations

0
Isolation of derivatives from the food-grade probiotic Lactobacillus johnsonii CNCM I-4884 with enhanced anti- Giardia activity DOI Creative Commons
Anne-Sophie Boucard, Saulius Kulakauskas,

Jana Alazzaz

et al.

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: March 27, 2025

Giardiasis, a widespread intestinal parasitosis affecting humans and animals, is growing concern due to the emergence of drug-resistant strains G. intestinalis. Probiotics offer promising alternative for preventing treating giardiasis. Recent studies have shown that probiotic Lactobacillus johnsonii CNCM I-4884 inhibits intestinalis growth both in vitro vivo. This protective effect largely mediated by bile salt hydrolase (BSH) enzymes, which convert conjugated acids (BAs) into free forms are toxic parasite. The objective this study was use adaptive evolution develop stress-resistant derivatives L. I-4884, with aim improving its anti-Giardia activity. Twelve enhanced resistance BAs reduced autolysis were generated. Among them, derivative M11 exhibited highest BSH Genomic proteomic analyses revealed two SNPs upregulation global stress response SigB, likely contributed increased overproduction. Finally, efficacy validated murine model In conclusion, our results demonstrate an effective strategy generate robust food-grade bacteria improved health benefits.

Language: Английский

Citations

0
Novel and diverse features identified in the genomes of bacteria isolated from a hydrothermal vent plume DOI Creative Commons
Samuel R. Major, Jennifer M. Polinski,

Kevin Penn

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

ABSTRACT Hydrothermal vent plumes (HVPs), formed by high-temperature emissions, are rich in compounds that support chemosynthesis and serve as reservoirs of microbial diversity genetic innovation. Through turbulence, mixing, interaction with subsea currents, communities thought to disperse across ocean basins. In this study, we focused on the plume Moytirra hydrothermal field, a relatively unexplored site, investigate its inhabitants. We cultured bacteria from HVP using 11 different media types performed complete genome sequencing 12 isolates. Our analyses revealed four putatively novel species Thalassobaculum , Sulfitobacter Idiomarina Christiangramia genera. Comparative genomics identified unique genomic islands containing biosynthetic gene clusters, including Non-Ribosomal Peptide Synthetase/Polyketide Synthase cluster, toxin-antitoxin systems, evidence horizontal transfer facilitated prophages. These findings underscore potential HVPs source biotechnologically relevant genes, contributing our understanding biodiversity complexity these extreme environments. IMPORTANCE vents dynamic environments offer nutrients for chemosynthetic organisms drive biology deep-sea. The dynamics ecosystems innovation resident populations. (HVPs) mix surrounding water, carrying local microbiota them dispersing hundreds kilometers. This study isolated capture snapshot community, revealing within three taxonomic classes. addition genomes public databases provides valuable insights into function, architecture, clusters found

Language: Английский

Citations

0