Methane emissions offset atmospheric carbon dioxide uptake in coastal macroalgae, mixed vegetation and sediment ecosystems

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Jan. 3, 2023

Coastal ecosystems can efficiently remove carbon dioxide (CO2) from the atmosphere and are thus promoted for nature-based climate change mitigation. Natural methane (CH4) emissions these may counterbalance atmospheric CO2 uptake. Still, knowledge of mechanisms sustaining such CH4 their contribution to net radiative forcing remains scarce globally prevalent macroalgae, mixed vegetation, surrounding depositional sediment habitats. Here we show that habitats emit in range 0.1 - 2.9 mg m-2 d-1 atmosphere, revealing situ macroalgae were sustained by divergent methanogenic archaea anoxic microsites. Over an annual cycle, CO2-equivalent offset 28 35% sink capacity attributed uptake vegetation habitats, respectively, augment release unvegetated sediments 57%. Accounting alongside sea-air fluxes identifying controlling is crucial constrain potential coastal as sinks develop informed mitigation strategies.

Language: Английский

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Mechanisms of extracellular electron transfer in anaerobic methanotrophic archaea

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 17, 2024

Abstract Anaerobic methanotrophic (ANME) archaea are environmentally important, uncultivated microorganisms that oxidize the potent greenhouse gas methane. During methane oxidation, ANME engage in extracellular electron transfer (EET) with other microbes, metal oxides, and electrodes through unclear mechanisms. Here, we cultivate ANME-2d ( ‘Ca . Methanoperedens’) bioelectrochemical systems observe strong methane-dependent current (91–93% of total current) associated high enrichment ‘ Ca Methanoperedens’ on anode (up to 82% community), as determined by metagenomics transmission microscopy. Electrochemical metatranscriptomic analyses suggest EET mechanism is similar at various electrode potentials, possible involvement an uncharacterized short-range transport protein complex OmcZ nanowires.

Language: Английский

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Sulfate concentrations affect sulfate reduction pathways and methane consumption in coastal wetlands

Water Research, Journal Year: 2022, Volume and Issue: 217, P. 118441 - 118441

Published: April 9, 2022

Language: Английский

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Borgs are giant genetic elements with potential to expand metabolic capacity

Nature, Journal Year: 2022, Volume and Issue: 610(7933), P. 731 - 736

Published: Oct. 19, 2022

Abstract Anaerobic methane oxidation exerts a key control on greenhouse gas emissions 1 , yet factors that modulate the activity of microorganisms performing this function remain poorly understood. Here we discovered extraordinarily large, diverse DNA sequences primarily encode hypothetical proteins through studying groundwater, sediments and wetland soil where production occur. Four curated, complete genomes are linear, up to approximately Mb in length share genome organization, including replichore structure, long inverted terminal repeats genome-wide unique perfect tandem direct intergenic or generate amino acid repeats. We infer these highly divergent archaeal extrachromosomal elements with distinct evolutionary origin. Gene sequence similarity, phylogeny local divergence composition indicate many their genes were assimilated from methane-oxidizing Methanoperedens archaea. refer as ‘Borgs’. identified at least 19 different Borg types coexisting spp. four ecosystems. Borgs provide archaea access encoding involved redox reactions energy conservation (for example, clusters multihaem cytochromes methyl coenzyme M reductase). These data suggest might have previously unrecognized roles metabolism group archaea, which known emissions, but further studies now needed establish functional relevance.

Language: Английский

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Organic carbon preservation in wetlands: Iron oxide protection vs. thermodynamic limitation

Water Research, Journal Year: 2023, Volume and Issue: 241, P. 120133 - 120133

Published: May 26, 2023

Language: Английский

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Pathways of methane removal in the sediment and water column of a seasonally anoxic eutrophic marine basin

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 10

Published: Jan. 26, 2023

Methane (CH 4 ) is a key greenhouse gas. Coastal areas account for major proportion of marine CH emissions. Eutrophication and associated bottom water hypoxia enhance production in coastal sediments. Here, we assess the fate produced sediments at site seasonally anoxic eutrophic basin (Scharendijke, Lake Grevelingen, Netherlands) spring (March) late summer (September) 2020. Removal sediment through anaerobic oxidation with sulfate (SO42-) known to be incomplete this system, as confirmed here by only slightly higher values δ 13 C-CH δD-CH porewater shallow sulfate-methane-transition zone (~5-15 cm depth) when compared deeper layers. In March 2020, column was fully oxygenated, that escaped from least partially removed aerobic oxidation. September below ~35 m depth, accumulated high concentrations (up 73 µmol L -1 waters oxycline. The sharp counter gradient oxygen depth increase above oxycline indicate mostly removal . Water profiles particulate dissolved Fe Mn suggest redox cycling both metals oxycline, pointing towards potential role metal oxides removal. id="im2">NH4+ id="im3">NO3- solutes near Analyses 16S rRNA gene sequences retrieved reveal presence oxidizing bacteria ( Methylomonadaceae methanotrophic archaea Methanoperedenaceae ), latter potentially capable id="im4">NO3- and/or metal-oxide dependent oxidation, Overall, our results combination pathways, which vary seasonally. Some appears escape surface atmosphere, however. We conclude eutrophication may make more important source atmosphere than commonly assumed.

Language: Английский

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Anaerobic oxidation of methane driven by different electron acceptors: A review

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 946, P. 174287 - 174287

Published: June 28, 2024

Language: Английский

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Towards an integrated view on microbial CH4, N2O and N2 cycles in brackish coastal marsh soils: A comparative analysis of two sites

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 918, P. 170641 - 170641

Published: Feb. 6, 2024

Coastal ecosystems, facing threats from global change and human activities like excessive nutrients, undergo alterations impacting their function appearance. This study explores the intertwined microbial cycles of carbon (C) nitrogen (N), encompassing methane (CH

Language: Английский

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Sulfide Toxicity as Key Control on Anaerobic Oxidation of Methane in Eutrophic Coastal Sediments

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(26), P. 11421 - 11435

Published: June 18, 2024

Coastal zones account for 75% of marine methane emissions, despite covering only 15% the ocean surface area. In these ecosystems, tight balance between production and oxidation in sediments prevents most from escaping into seawater. However, anthropogenic activities could disrupt this balance, leading to an increased escape coastal sediments. To quantify unravel potential mechanisms underlying disruption, we used a suite biogeochemical microbiological analyses investigate impact anthropogenically induced redox shifts on cycling three sites with contrasting bottom water conditions (oxic-hypoxic-euxinic) eutrophic Stockholm Archipelago. Our results indicate that under hypoxia euxinia, while anaerobic was disrupted euxinia. Experimental, genomic, data suggest virtual disappearance methane-oxidizing archaea at euxinic site occurred due sulfide toxicity. This explain near 7-fold increase extent benthic relative hypoxic one. conclusion, insights reveal how development euxinia biofilter, potentially emissions zones.

Language: Английский

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Active anaerobic methane oxidation and sulfur disproportionation in the deep terrestrial subsurface

The ISME Journal, Journal Year: 2022, Volume and Issue: 16(6), P. 1583 - 1593

Published: Feb. 16, 2022

Abstract Microbial life is widespread in the terrestrial subsurface and present down to several kilometers depth, but energy sources that fuel metabolism deep oligotrophic anoxic environments remain unclear. In crystalline bedrock of Fennoscandian Shield at Olkiluoto, Finland, opposing gradients abiotic methane ancient seawater-derived sulfate create a sulfate-methane transition zone (SMTZ). We used chemical isotopic data coupled genome-resolved metaproteogenomics demonstrate active and, for first time, provide direct evidence anaerobic oxidation (AOM) bedrock. Proteins from Methanoperedens (formerly ANME-2d) are readily identifiable despite low abundance (≤1%) this genus confirm occurrence AOM. This finding supported by 13C-depleted dissolved inorganic carbon. Desulfocapsaceae Desulfurivibrionaceae, addition 34S-enriched sulfate, suggest these organisms use sulfur compounds as both electron donor acceptor. Zerovalent groundwater may derive rock interactions, or non-obligate syntrophy with Methanoperedens, potentially linking cycles Olkiluoto groundwater. Finally, putative episymbionts candidate phyla radiation (CPR) DPANN archaea represented significant diversity (26/84 genomes) roles carbon cycling. Our results highlight AOM disproportionation metabolisms show microbial activity subsurface.

Language: Английский

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