Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(7), P. 6535 - 6535

Published: March 31, 2023

Microbial contamination in the hospital environment is a major concern for public health, since it significantly contributes to onset of healthcare-associated infections (HAIs), which are further complicated by alarming level antimicrobial resistance (AMR) HAI-associated pathogens. Chemical disinfection control bioburden has temporary effect and can favor selection resistant pathogens, as observed during COVID-19 pandemic. Instead, probiotic-based sanitation (probiotic cleaning hygiene system, PCHS) was reported stably abate AMR, HAIs. PCHS action not rapid nor specific, being based on competitive exclusion, but addition lytic bacteriophages that quickly specifically kill selected bacteria shown improve effectiveness. This study aimed investigate such combined probiotic-phage (PCHSφ) two Italian hospitals, targeting staphylococcal contamination. The results showed PCHSφ could provide higher removal staphylococci, including strains, compared with disinfectants (-76%, p < 0.05) alone (-50%, 0.05). Extraordinary sporadic chlorine appeared compatible PCHSφ, while frequent routine usage inactivated probiotic/phage components, preventing action. collected data highlight potential biological better infectious risk healthcare facilities, without worsening pollution AMR concerns.

Language: Английский

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Estimation, Evaluation and Characterization of Carbapenem Resistance Burden from a Tertiary Care Hospital, Pakistan

Antibiotics, Journal Year: 2023, Volume and Issue: 12(3), P. 525 - 525

Published: March 6, 2023

Carbapenem resistance has become major concern in healthcare settings globally; therefore, its monitoring is crucial for intervention efforts to halt spread. During May 2019-April 2022, 2170 clinical strains were characterized antimicrobial susceptibility, genes, replicon and sequence types. Overall, 42.1% isolates carbapenem-resistant, significantly associated with Klebsiella pneumoniae (K. pneumoniae) (p = 0.008) Proteus species 0.043). Carbapenemases detected 82.2% of isolates, blaNDM-1 (41.1%) the ICU < 0.001), cardiology 0.042), pediatric medicine 0.013) wound samples 0.041); blaOXA-48 (32.6%) was 0.008), general surgery 0.005) nephrology 0.020); blaKPC-2 (5.5%) 0.029); blaNDM-1/blaOXA-48 (11.4%) 0.002), urine 0.003) blood 0.012) samples; blaOXA-48/blaVIM (3.1%) 0.001) 0.001). Other carbapenemases blaVIM (3.0%), blaIMP (2.7%), blaOXA-48/blaIMP (0.1%) blaVIM/blaIMP (0.3%). Sequence type (ST)147 (39.7%) represented most common identified among K. pneumoniae, along ST11 (23.0%), ST14 (15.4%), ST258 (10.9%) ST340 (9.6%) while ST405 comprised 34.5% Escherichia coli (E. coli) followed by ST131 (21.2%), ST101 (19.7%), ST10 (16.0%) ST69 (7.4%). Plasmid types IncFII, IncA/C, IncN, IncL/M, IncFIIA IncFIIK observed. This first report describing carbapenem-resistance burden emergence blaKPC-2-ST147, blaNDM-1-ST340 blaNDM-1-ST14 blaNDM-1-ST69 blaNDM-1/blaOXA-48-ST69 E. coharboring extended-spectrum beta-lactamases (ESBLs) from Pakistan.

Language: Английский

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Potential sources of contamination on textiles and hard surfaces identified as high-touch sites near the patient environment

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(7), P. e0287855 - e0287855

Published: July 7, 2023

The hospital environment represents an important mediator for the transmission of healthcare-associated infections through direct and indirect hand contact with hard surfaces textiles. In this study, bacteria on high-touch sites, including textiles in two care wards Sweden, were identified using microbiological culture methods 16S rDNA sequencing. During a cross-sectional 176 further analysed quantification total aerobic bacteria, Staphylococcus aureus, Clostridium difficile Enterobacteriacae. bacterial population structures 26 samples study showed higher frequency unique hand-textile contacts (36 per hour), compared to (2.2 hour). Hard met recommended standard ≤ 5 CFU/cm2 1 S. aureus (53% 35%, respectively) extent (19% 30%, (P = 0.0488). number genera was than surfaces. (30.4%) Corynebacterium (10.9%) most representative Streptococcus (13.3%) fact that big percentage did not fulfil criteria cleanliness, combined diversity, surfaces, are indicators reservoirs potential risk vectors transmission. However, since found belonged normal flora, it possible draw conclusions as sources healthcare associated infections.

Language: Английский

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Charge-specific and hesperidin loaded mesoporous silica nanoparticles: Enhanced antimicrobial activity against pathogenic bacteria

Nano-Structures & Nano-Objects, Journal Year: 2025, Volume and Issue: 41, P. 101441 - 101441

Published: Jan. 18, 2025

Language: Английский

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Comprehensive Investigation on Synthesis, Computational, Antioxidant, Antimicrobial, and Bio-imaging Studies of Salicylaldehyde-based Schiff Bases

Chemical Data Collections, Journal Year: 2025, Volume and Issue: unknown, P. 101184 - 101184

Published: Feb. 1, 2025

Language: Английский

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