Biocontrol of Agroathelia rolfsii associated with stem rot disease in tomato (Solanum lycopersicum L.) and growth promotion using compost-associated actinobacteria

Brazilian Journal of Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Language: Английский

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Optimizing the batch fermentation process (agitation and aeration) of the biocontrol agent, Bacillus velezensis strain KSAM1, and its influence on soilborne fungus, Macrophomina phaseolina

Journal of King Saud University - Science, Journal Year: 2025, Volume and Issue: 0, P. 1 - 12

Published: Feb. 28, 2025

Language: Английский

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Bioactivities evaluation of an endophytic bacterial strain Bacillus velezensis JRX-YG39 inhabiting wild grape

BMC Microbiology, Journal Year: 2022, Volume and Issue: 22(1)

Published: July 2, 2022

Abstract Background Botrytis cinerea can cause serious disease on lots of plant hosts during growth and postharvest storage. Biocontrol is known to be eco-friendly methods control pathogens. Plant endophytic bacteria are generally considered as beneficial organisms, since they promote enhance immune system. Thus, screening biological agents very important for sustainable protection. Results Fifty-six were obtained from wild grape. Sixteen isolates their extracts exhibited significant antifungal activity against B. . Particularly, strain JRX-YG39 with the strongest inhibition ability had a broad-spectrum activity. Combining 16S rDNA analysis phylogenetic results based gyr A B genes, was assigned Bacillus velezensis could produce bioactive VOCs obviously depressed mycelia It confirmed that released by significantly induce defense Arabidopsis thaliana Thirty-one secondary metabolites further identified culture gas chromatography–mass spectrometry analysis. Dibutyl phthalate, potential substance, major compound accounting 78.65%. Conclusions has wide antagonistic promotion Hence, will provide valuable constituent modern agricultural practice biofertilizers biocontrol agents.

Language: Английский

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Marine actinomycetes for biocontrol of Fusarium solani in tomato plants: In vitro and in vivo studies

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Journal Year: 2024, Volume and Issue: 52(2), P. 13562 - 13562

Published: June 27, 2024

Using microorganisms as biocontrol agents of phytopathogens has been an alternative to synthetic fungicides. Actinomycetes isolated from soil and plants have reduced diseases caused by phytopathogens; however, marine environments may be option agents. The tomato crop possesses important economic impact worldwide, being Mexico the main exporter. Several species Fusarium cause damage crops are controlled with objective this work was determine effect actinomycetes on solani in plants. Four strains (A20, A19, A18, A15) one terrestrial actinomycete (ED48) were used. used, produced siderophores. greatest inhibition mycelial growth F. due iron competition obtained strain A19 74.28%. Only two showed antifungal activity VOCs (A19 A18), highest antagonistic PICR 76.75%. treatments significant differences fungicide application growth, disease severity (SE), incidence (DI) variables. (A19) infested increased levels enzyme (SOD, POD, CAT, PAL) versus that only distilled water (control) applied. origin for solani.

Language: Английский

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Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: July 22, 2022

Gray mold caused by Botrytis cinerea is detrimental to plants and fruits. Endophytes have been shown modify plant disease severity in functional assays. We conducted this study investigate the endophytic strain Bacillus K1 with excellently antagonistic B. from wild grape endosphere. identified a high antifungal activity against both vitro vivo. Combining phylogenetic results based on 16S rDNA genome sequencing, was assigned as subtilis. The displayed that its volatile substances could significantly inhibit mycelia growth of cinerea. Grape fruit inoculated showed lower gray during treatment. higher levels defense-related enzymes, including peroxidase, polyphenol oxidase, phenylalanine ammonia lyase, were induced grapes after inoculation. Scanning electron microscopy (SEM) suggested inhibited mycelial via bacterial colonization antibiosis grapes. gas chromatography-mass spectrometry analysis 33 volatiles which dibutyl phthalate major compound accounting for 74.28%. Dibutyl demonstrated strong suppressing Genome bioinformatics revealed chromosome harbored many known biosynthesis gene clusters encoding subtilosin, bacillaene, bacillibactin, bacilysin, fengycin. This provides potential biological agent control diseases post-harvest improves our understanding possible biocontrol mechanisms strain.

Language: Английский

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Cadmium-tolerant Bacillus cereus 2–7 alleviates the phytotoxicity of cadmium exposure in banana plantlets

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 903, P. 166645 - 166645

Published: Aug. 30, 2023

Language: Английский

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Combatting Sugar Beet Root Rot: Streptomyces Strains’ Efficacy against Fusarium oxysporum

Plants, Journal Year: 2024, Volume and Issue: 13(2), P. 311 - 311

Published: Jan. 20, 2024

Sugar beet root rot disease triggered by Fusarium oxysporum f. sp. radicis-betae is a destructive and dramatically affects the production quality of sugar industry. Employing beneficial microorganisms as biocontrol strategy represents an eco-friendly sustainable approach to combat various plant diseases. The distinct aspect this study was assess antifungal growth-promoting capabilities recently isolated Streptomyces treat plants against infection with phytopathogen F. oxysporum. Thirty-seven actinobacterial isolates were recovered from rhizosphere healthy screened for their potential antagonize in vitro. Two SB3-15 SB2-23 that displayed higher antagonistic effects morphologically molecularly identified spp. Seed treatment fermentation broth selected strains significantly reduced severity compared infected control greenhouse experiment. exhibited highest protective activity high efficacy ranging 91.06 94.77% chemical fungicide (86.44 92.36%). Furthermore, strain increased weight, length, diameter. Likewise, it improves sucrose percentage juice purity. As consequence, SB2-23's intriguing capability growth stimulation present promising prospects its utilization both protection enhancement strategies.

Language: Английский

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Rhizosphere actinobacteria isolated from Pometia pinnata and its antimicrobial activity

Biodiversitas Journal of Biological Diversity, Journal Year: 2024, Volume and Issue: 25(3)

Published: March 23, 2024

Abstract. Janatiningrum I, Zahra A, Fitriyanti, Anggia V. 2024. Rhizosphere actinobacteria isolated from Pometia pinnata and its antimicrobial activity. Biodiversitas 25: 1007-1014. Actinobacteria are currently the largest antibiotic-producing bacteria due to their extreme environments such as soil, roots rhizosphere. The diversity of rhizosphere is affected by plant exudates. a that has long been used for traditional medicine in Indonesia. This study was aimed at isolating evaluating isolate evaluate P. pinnata, screen antibacterial antifungal properties. Isolation done using serial dilution method grown on media. About 34 were successfully 50% them showed index activity Staphylococcus aureus ATCC 6538 (0.52), Streptococcus pneumoniae 1705 (2.87), Escherichia coli 25922 (1.28). Several microbial microbes (20.8%) found capable inhibiting fungal growth. highest against all shown RM12 isolate. aqueous fraction demonstrated significant inhibitory S. E. concentrations 250 ppm, 500 ppm 1000 ppm. TLC bioautography RM 12 do not belong polyphenol or phenol compound group. characterized 16S ribosomal RNA sequence genera Streptomyces bungoensis.

Language: Английский

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Antarctic Streptomyces: Promising biocontrol agents for combating Fusarium oxysporum f. sp. cubense

Biotechnology Reports, Journal Year: 2024, Volume and Issue: 43, P. e00852 - e00852

Published: Aug. 23, 2024

Fusarium wilt of Banana (FWB) caused by oxysporum f. sp. cubense (Foc) poses a significant threat to the banana industry, with current inadequate control measures. This study evaluated antifungal potential nine Streptomyces strains isolated from Antarctic soil samples, using Casein-Starch media stimulate production compounds. The inhibition spectrum against Foc was assessed under laboratory conditions well diffusion on Mueller-Hinton agar, activity measured in arbitrary units (AU/mL) and minimum inhibitory concentration (MIC) tested ethyl acetate extracts. Among isolates, K6 E7 were closely related polyrhachis fildesensis, exhibited activity, showing 320 80 AU/mL, MIC values 250 >500 ppm, respectively. These findings highlight as biocontrol agents Foc, offering new avenues for sustainable management cultivation.

Language: Английский

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Antimicrobial Potential of Streptomyces spp. Isolated from the Rift Valley Regions of Ethiopia

Advances in Pharmacological and Pharmaceutical Sciences, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 10

Published: June 13, 2022

The study was undertaken to isolate, screen, and identify actinomycetes with antimicrobial metabolites. Twenty-one composite soil samples were randomly collected from various unique agroecological niches in the Rift Valley of Ethiopia. serially diluted spread on starch casein agar medium supplemented 50 μg/ml cycloheximide 25 nalidixic acid. Two hundred forty-nine (249) cultures isolated screened by cross streaking against human pathogens. Twenty-four isolates pronounced activity selected for identification further screening. Among isolates, 172 (69.1%) showed activities tested inhibition zone ranged 5 ± 0.31 >40 mm during primary crude extracts promising a statistically significant difference $\left(P<0.05\right)$ between them control. RVE129 RVE217 maximum at 27 0.6 26 mm, respectively, S. aureus, results higher than standard drug streptomycin (25 0.58 mm). 22 0.0 id="M2"> . aeruginosa, almost comparable (24 Crude extract RVE187 zones 16 0.33 A. niger ATCC10535, which, however, smaller those obtained amphotericin B (29 actinomycete strains remarkable bioactivity characterized using cultural, morphological, physiological, biochemical characteristics assigned under genus Streptomycetes. finding current indicates that Streptomyces sp. Ethiopia found possess broad spectrum range

Language: Английский

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