Bacillus VOCs in the Context of Biological Control

Antibiotics, Journal Year: 2023, Volume and Issue: 12(3), P. 581 - 581

Published: March 15, 2023

A contemporary agricultural production system relying on heavy usage of agrochemicals represents a questionable outlook for sustainable food supply in the future. The visible negative environmental impacts and unforeseen consequences to human animal health have been requiring shift towards novel eco-friendly alternatives chemical pesticides while now. Microbial-based biocontrol agents shown promising potential plant disease management. bacteria genus Bacillus among most exploited microbial active components due several highly efficient mechanisms action against pathogens, as well palette additional plant-beneficial mechanisms, together with their suitable properties biopesticide formulations. Among other bioactive metabolites, volatile organic compounds (VOCs) investigated applications, exhibiting main advantage long-distance effect without necessity direct contact plants or pathogens. aim this study is give an overview state-of-the-art field Bacillus-based VOCs, especially terms antibacterial, antifungal, nematicidal segments determining applications agriculture.

Language: Английский

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Novel Approaches for Sustainable Horticultural Crop Production: Advances and Prospects

Horticulturae, Journal Year: 2022, Volume and Issue: 8(10), P. 910 - 910

Published: Oct. 5, 2022

Reduction of plant growth, yield and quality due to diverse environmental constrains along with climate change significantly limit the sustainable production horticultural crops. In this review, we highlight prospective impacts that are positive challenges for application beneficial microbial endophytes, nanomaterials (NMs), exogenous phytohormones strigolactones (SLs) new breeding techniques (CRISPR), as well controlled environment horticulture (CEH) using artificial light in The benefits such applications often evaluated by measuring their impact on metabolic, morphological biochemical parameters a variety cultures, which typically results higher yields efficient use resources when applied greenhouse or field conditions. Endophytic microbes promote growth play key role adapting plants habitat, thereby improving prolonging protection from biotic abiotic stresses. Focusing control, considered effects novel class SLs, NMs CEH commodities. addition, genomic editing CRISPR, including its modulating gene expression/transcription factors crop tolerance, was also reviewed.

Language: Английский

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Unleashing Bacillus species as versatile antagonists: Harnessing the biocontrol potentials of the plant growth-promoting rhizobacteria to combat Macrophomina phaseolina infection in Gloriosa superba

Microbiological Research, Journal Year: 2024, Volume and Issue: 283, P. 127678 - 127678

Published: March 6, 2024

Charcoal rot caused by Macrophomina phaseolina is one of the most devastating diseases that cause severe yield loss in Gloriosa superba cultivation. Plant growth-promoting rhizobacteria (PGPR) are extensively harnessed as biocontrol agents due to their effectiveness combating a wide array plant pathogens through multifaceted approach. The present study delved into mechanisms underlying its ability inhibit root pathogen and capacity promote growth G. superba, commonly known glory lily. PGPR isolated from rhizosphere lily were subjected vitro assessments using dual plate technique. Bacillus subtilis BGS-10 B. velezensis BGS-21 showed higher mycelial inhibition (61%) against M. phaseolina. These strains also producing indole-3-acetic acid, siderophore, ammonia, amylase, cellulase, pectinase, xylanase, lipase chemicals. Genome screening revealed presence antimicrobial peptide genes such Iturin (ituD gene), surfactin (srfA sfp genes) along with mycolytic enzyme β-1,3-glucanase. Further, secondary metabolites bacterial secretome was identified gas chromatography-mass spectrometry (GC/MS) analysis. Notably, pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl), 9 H-pyrido[3,4-b] indole L-leucyl-D-leucine exhibited highest docking score enzymes responsible for cell wall degradation. Under glasshouse conditions, tuber treatment soil application talc-based formulation suppress incidence minimal disease 27.78% over untreated control. Concurrently, there notable induction defense-related enzymes, including peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), Therefore, it can be concluded play significant role fortifying plant's defense pathogen.

Language: Английский

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Research progress of volatile organic compounds produced by plant endophytic bacteria in control of postharvest diseases of fruits and vegetables

World Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 39(6)

Published: April 6, 2023

Language: Английский

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Current insights into the biocontrol and biotechnological approaches for postharvest disease management of Botrytis cinerea

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 216, P. 113055 - 113055

Published: June 18, 2024

Language: Английский

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Endophyte mediated biocontrol mechanisms of phytopathogens in agriculture

Research in Microbiology, Journal Year: 2024, Volume and Issue: 175(8), P. 104229 - 104229

Published: July 9, 2024

Language: Английский

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Bacillus velezensis R22 inhibits the growth of multiple fungal phytopathogens by producing surfactin and four fengycin homologues

Biotechnology & Biotechnological Equipment, Journal Year: 2024, Volume and Issue: 38(1)

Published: Feb. 1, 2024

Significant agricultural losses are caused by the phytopathogenic fungi Botrytis cinerea and Phytophthora infestans, as well bacteria of Ralstonia solanacearum species. The present work aimed to isolate rhizobacteria for simultaneous biocontrol these three species suggest mechanisms their antagonistic action. Among 120 Bacillus spp. isolated from soils, velezensis licheniformis strains displayed highest activity against all phytopathogens. A rapid, polymerase chain reaction-based method detecting nonribosomal peptide synthetase genes was developed elucidate genetic basis traits. presence fenA, srfAA, ppsA, lchAA genes, encoding fengycin/surfactin/plipastatin synthetases lichenysin synthase, revealed in strains’ genomes. whole genome sequencing (WGS) B. R22 showed that it contains 4,081,504 bp (with G + C content 46.35%), 4087 3935 proteins, 72 tRNAs, 14 rRNAs, 5 ncRNAs. WGS allowed prediction 10 complete clusters secondary metabolites with putative antimicrobial activity: difficidin, fengycin, bacillaene, butyrosin, bacillibactin, bacilysin, surfactin, macrolactin H, R22, velezensin. LC-MS high-sensitivity UHPLC-Q-TOF LC-MS/MS analysis were used search predicted cell-free supernatants R22. compounds strongest antifungal surfactin a C15 β-OH fatty acid chain; two homologous forms fengycin A; B homologues containing C16 C17 β-hydroxy chains. broad spectrum its molecular characterization provide good future development plant protection preparations.

Language: Английский

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In Vitro Evaluation of Some Endophytic Bacillus to Potentially Inhibit Grape and Grapevine Fungal Pathogens

Plants, Journal Year: 2023, Volume and Issue: 12(13), P. 2553 - 2553

Published: July 5, 2023

Romania has a long history of grapevine culturing and winemaking. However, like any agricultural sector, viticulture faces devastating biological threats. Fungi responsible for trunk diseases (GTDs) grape spoilage lead to considerable yield losses decline in quality. In the actual context, many countries, including Romania, have reoriented their approaches minimize chemical inputs, which been proven be toxic negative impacts on environment, replace them with sustainable biocontrol strategies wine-growing sector. Within strategies, Bacillus spp. is well-known plant-protective bacteria antifungal properties. this paper, six endophytic from various plant sources were studied. The bacterial strains identified as B. pumilus, subtilis, velezensis by sequencing 16S rDNA region. Regardless vitro test methods (using living cells, bacterial-cell-free supernatant (CFS), volatile active compounds (VOCs)), revealed strong broad activity against fungal pathogens such Aspergillus spp., Botrytis cinerea, Penicillium expansum, Diplodia seriata, Eutypa lata, Fusarium Clonostachys rosea, Neofusicoccum parvum, Stereum hirsutum. functional genes encoding difficidin, fengycin, iturins, macrolactin, mycosubtilin molecularly detected, could support strains. Lytic enzymes involved growth inhibition, chitinase, cellulase, proteases, also produced some these Various other tests, phosphate phytate solubilization, phytohormone synthesis, production polyamine biosynthetic pathway, pH well temperature tolerance tests carried out reveal plant-beneficial potential These results that strains, especially BAHs1, are most suitable endophytes biologic control, future development management strategies.

Language: Английский

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Screening, and Optimization of Fermentation Medium to Produce Secondary Metabolites from Bacillus amyloliquefaciens, for the Biocontrol of Early Leaf Spot Disease, and Growth Promoting Effects on Peanut (Arachis hypogaea L.)

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(11), P. 1223 - 1223

Published: Nov. 20, 2022

A novel Bacillus amyloliquefaciens BAM strain, with fermentation nutrient mediums and compositions, could produce potent antifungal secondary metabolites, as the existing strains face resistance from fungus pathogens. In current study, we introduced two for process, semolina peanut root extract, carbon nitrogen sources in order to maximize effects of B. against Cercaspora arachidichola control early leaf spot disease peanuts. Based on a single-factor test central composite design response surface methodology, optimum medium antagonistic substance was determined, containing 15 gm/L flour, 12.5 beef 0.5 magnesium sulfate, which inhibited fungal growth by 91%. vitro, activity showed that broth optimized formulation had an inhibition rate (92.62 ± 2.07)% C. arachidichola. Disease pot experiments show pre-infection spray significant efficiency (92.00 3.79)% comparison post-infection spray. significantly promoted plant physiological parameters reduced biotic stress archidechola. Studies revealed be ideal biocontrol biofertilizer agent help management spots

Language: Английский

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Effect of volatile compounds produced by Weissella cibaria BWL4 on Botrytis cinerea infection in fruit and complete genome sequence analysis of BWL4

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 213, P. 112917 - 112917

Published: April 1, 2024

Language: Английский

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