Chemical Communications,
Journal Year:
2023,
Volume and Issue:
59(90), P. 13434 - 13437
Published: Jan. 1, 2023
The
improper
disposal
of
hospital
waste
products
containing
genetic
materials
poses
a
serious
safety
threat.
We
present
herein
an
environmentally
friendly
technology
using
graphene-based
novel
carbon-allotropic
surface
to
remediate
such
wastes.
used
carbon-allotrope
is
decorated
with
enediyne
(EDE-1)
enriched
aromatic
pi-conjugated
structure
create
efficient
and
active
for
cleaving
DNA
strands.
Under
controlled
exposure
ultraviolet
(UV)
radiation
heat,
the
developed
influences
degradation
without
disturbing
bacterial
populations
downstream
water
treatment
system.
designed
material
has
been
extensively
characterized
physicochemical
biological
tools.
Our
results
indicate
that
this
approach
can
possibly
be
introduced
in
large
scale
streams
remediating
hazards
thereby
developing
portable
self-contained
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: March 15, 2024
Abstract
Wastewater
treatment
plants
(WWTPs)
are
considered
to
be
hotspots
for
the
spread
of
antibiotic
resistance
genes
(ARGs).
We
performed
a
metagenomic
analysis
raw
wastewater,
activated
sludge
and
treated
wastewater
from
two
large
WWTPs
responsible
urban
in
Moscow,
Russia.
In
untreated
several
hundred
ARGs
that
could
confer
most
commonly
used
classes
antibiotics
were
found.
employed
nitrification/denitrification
or
an
anaerobic/anoxic/oxic
process
enabled
efficient
removal
organic
matter,
nitrogen
phosphorus,
as
well
fecal
microbiota.
The
resistome
constituted
about
0.05%
whole
metagenome,
after
water
its
share
decreased
by
3–4
times.
resistomes
dominated
encoding
beta-lactams,
macrolides,
aminoglycosides,
tetracyclines,
quaternary
ammonium
compounds,
sulfonamides.
macrolides
tetracyclines
removed
more
efficiently
than
beta-lactamases,
especially
ampC
,
abundant
ARG
effluent.
efficiency
particular
was
impacted
technology.
Metagenome-assembled
genomes
multidrug-resistant
strains
assembled
both
influent
Ccomparison
Moscow
around
world
suggested
abundance
content
depend
on
social,
economic,
medical,
environmental
factors.
Frontiers in Synthetic Biology,
Journal Year:
2025,
Volume and Issue:
2
Published: Jan. 7, 2025
Developing
strategies
for
the
prevention
of
antimicrobial
resistance
genes
(ARG)
in
wastewater
treatment
facilities,
such
as
municipal
sewage
lagoons,
is
important
to
prevent
ARG
introduction
and
proliferation
environment.
While
previous
studies
have
shown
promising
results
reducing
ARGs
through
biological
phytoremediation
systems
like
Constructed
Wetlands,
underlying
mechanisms
impact
water
chemistry
on
reduction
remain
poorly
understood.
This
study
used
metagenomics
investigate
effect
three
different
treatments
microbial
community
resistome.
The
indicated
that
each
significantly
reduced
metal
(MRGs).
A
network
analysis
identified
eight
bacterial
genera
strongly
associated
with
genes,
six
which
belonged
phylum
Pseudomonadota
two
Campylobacteria.
Of
Pseudomonadota,
four
were
facultative
anaerobes.
strong
correlation
was
observed
between
iron
(Fe)
concentration
total
abundance
(
r
=
0.95),
well
(across
measured
metals)
0.93).
Additionally,
there
a
significant
Fe
0.86).
implies
high
concentrations
may
favor
ARG-harboring
bacteria
by
providing
an
electron
donor
anaerobic
respiration.
suggests
phytoextraction
metals,
including
Fe,
play
role
treated
columns.
These
findings
offer
valuable
insights
into
how
removal
can
effectively
reduce
levels
wastewater.
Environmental Microbiome,
Journal Year:
2024,
Volume and Issue:
19(1)
Published: April 29, 2024
Abstract
Background
Plastics
pollution
and
antimicrobial
resistance
(AMR)
are
two
major
environmental
threats,
but
potential
connections
between
plastic
associated
biofilms,
the
‘plastisphere’,
dissemination
of
AMR
genes
not
well
explored.
Results
We
conducted
mesocosm
experiments
tracking
microbial
community
changes
on
surfaces
transitioning
from
wastewater
effluent
to
marine
environments
over
16
weeks.
Commonly
used
plastics,
polypropylene
(PP),
high
density
polyethylene
(HDPE),
low
(LDPE)
terephthalate
(PET)
incubated
in
effluent,
river
water,
estuarine
seawater
for
weeks,
were
analysed
via
16S
rRNA
gene
amplicon
shotgun
metagenome
sequencing.
Within
one
week,
plastic-colonizing
communities
shifted
effluent-associated
microorganisms
taxa,
some
members
which
(e.g.
Oleibacter-Thalassolituus
Sphingomonas
spp.,
PET,
Alcanivoracaceae
PET
PP,
or
Oleiphilaceae,
all
polymers),
selectively
enriched
levels
undetectable
starting
communities.
Remarkably,
biofilms
also
susceptible
parasitism,
with
Saprospiraceae
feeding
at
late
colonisation
stages
(from
week
6
onwards),
while
Bdellovibrionaceae
prominently
present
HDPE
2
LDPE
day
1.
Relative
abundance
declined
time,
plastics
did
become
key
after
exposure.
Conclusion
Although
occurred
during
transition
substrata,
those
originated
organisms.
Overall,
act
as
hotspots
proliferation
simulated
environments.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: Jan. 25, 2024
Introduction
In
modern
times,
bacterial
infections
have
become
a
growing
problem
in
the
medical
community
due
to
emergence
of
antibiotic-resistant
bacteria.
fact,
overuse
and
improper
disposal
antibiotics
led
resistance
presence
such
bacteria
wastewater.
Therefore,
it
is
critical
develop
effective
strategies
for
dealing
with
Electroporation
has
been
found
be
one
most
promising
complementary
techniques
inactivation
because
against
wide
range
bacteria,
non-chemical
highly
optimizable.
Many
studies
demonstrated
electroporation-assisted
but
rarely
clinical
or
resistant
these
used
study.
motivation
our
study
was
use
treatment
regimen
that
combines
electroporation
inactivate
Methods
We
separately
combined
two
(tetracycline
chloramphenicol)
which
are
(with
different
mode)
electric
pulses.
three
concentrations
(40,
80
150
µg/ml
tetracycline
100,
500
2000
chloramphenicol,
respectively)
four
field
strengths
(5,
10,
15
20
kV/cm)
electroporation.
Results
discussion
Our
results
show
effectively
enhances
effect
inactivates
The
rate
chloramphenicol
increase
strength
pulsed
and/or
concentration
antibiotic.
addition,
we
longer
lasting
(up
24
hours),
making
vulnerable
considerable
time.
present
work
provides
new
insights
into
aquatic
environment.
Plasma,
Journal Year:
2024,
Volume and Issue:
7(2), P. 284 - 299
Published: April 10, 2024
A
detailed
review
of
the
scientific
literature
was
undertaken
to
examine
most
recent
developments
in
plasma
processing
field
medicine.
The
first
part
includes
a
breakdown
different
types
coatings
that
can
be
applied
onto
medical
devices
using
plasma,
with
specific
focus
on
antimicrobial
surfaces.
plasma-deposited
biocompatibles,
drug
delivery
and
adhesive
2023
are
described,
applications
additive
manufacturing
highlighted.
use
plasma-activated
liquids
as
standalone
therapeutics
continues
evolve,
pertinent
advances
this
described.
In
addition,
combination
medicine
conventional
pharmaceutical
interventions
is
reviewed,
key
emerging
trends
highlighted,
including
enhance
directly
into
tissue.
potential
synergies
between
chemotherapeutics
for
oncology
infection
treatment
growing
area,
advancements
noted.
Finally,
control
excess
antibiotics
intentionally
degrade
such
materials
waste
streams
In
recent
decades,
antibiotic
resistance
has
become
a
major
global
health
threat.
This
study
evaluates
the
efficiency
of
two
wastewater
treatments
conventional
activated
sludge
and
advanced
filtration-based
Enviro-Septic
system
for
removing
genes
(ARGs)
their
environmental
prevalence
in
an
Algerian
watershed.
A
total
35
samples
122
watershed
were
collected
from
six
sites
along
Oued
Boussellam
River,
heavily
affected
by
sewage
effluent.
Sampling
covered
50
km
transect,
low-pollution
site
to
water
reservoir
used
production.
The
analyzed
fecal
bacterial
indicators
(E.
coli
spores
sulfite-reducing
clostridia),
viral
(somatic
coliphages
F-specific
RNA
phages),
CrAssphage,
three
ARGs
(blaTEM,
tetW,
sul1).
treatment
plant
(WWTP)
achieved
reductions
~4
log10
E.
somatic
coliphages,
3.5
1
spores.
showed
similar
efficacy
but
was
less
effective
(2.8
log10)
CrAssphage
(2.5
log10).
reduced
~3
both
systems.
All
river
tested
positive
ARGs,
with
lower
concentrations
at
fecally
polluted
(1,
5,
6),
showing
reduction
up
4
units
highest
(2)
lowest
(6).
Strong
correlations
observed
between
culturable
indicators,
(ρ
0.58–0.96),
indicating
that
detected
predominantly
linked
human
pollution.
offers
insights
into
ARG
dynamics
supports
development
strategies
mitigate
spread,
protecting
public
efficacy.
Biomedicines,
Journal Year:
2025,
Volume and Issue:
13(3), P. 534 - 534
Published: Feb. 20, 2025
Background/Objective:
Anti-tumor
effects
of
plasma-activated
medium
(PAM)
were
demonstrated
using
various
malignant
tumors.
However,
the
anti-tumor
effect
PAM
on
synovial
sarcoma
remains
unclear.
Therefore,
we
aimed
to
investigate
and
its
underlying
mechanisms,
focusing
quantitative
analyses
both
intracellular
reactive
oxygen
species
(ROS)
cell
apoptosis.
Methods:
The
human
line
HS-SY-II
was
used
viability
after
treatment.
We
investigated
side
local
injection
in
a
xenograft
murine
model.
Moreover,
observed
PAM-induced
ROS
accumulation
apoptosis
assessed
involvement
an
scavenger.
Results:
significantly
decreased
cells
compared
with
untreated
Dulbecco’s
modified
Eagle
medium.
Local
into
model
suppressed
tumor
growth,
including
volume
(p
<
0.001)
weight
=
0.031),
without
effects.
Regarding
induced
significant
0.001).
scavenger
inhibited
Conclusions:
confirmed
vitro
vivo,
as
well
absence
mechanism
suggested
involve
by
accumulation.
Considering
clinical
issues
associated
existing
treatments
sarcoma,
is
promising
new
option.
