Apmis,
Journal Year:
2024,
Volume and Issue:
132(12), P. 948 - 955
Published: Oct. 6, 2024
Endotoxemia
is
closely
related
to
many
diseases.
As
the
largest
endotoxin
reservoir
in
human
body,
gut
microbiota
should
be
a
key
target
for
alleviating
endotoxemia.
The
intestinal
believed
cause
endotoxemia
directly
or
indirectly
by
modifying
barrier
function
through
dysbiosis,
changing
mucosal
permeability
and
bacterial
translocation.
Diet
known
main
environmental
factor
affecting
microbiota,
different
diets
food
components
have
large
impact
on
microbiota.
Mediterranean
diet,
which
received
much
attention
recent
years,
able
regulate
thereby
maintaining
of
In
this
review,
we
focus
relationship
between
endotoxemia,
how
dietary
(MD)
pattern
can
interfere
with
Food & Function,
Journal Year:
2023,
Volume and Issue:
14(23), P. 10401 - 10417
Published: Jan. 1, 2023
Gut
microbiota
are
closely
related
to
lipopolysaccharide
(LPS)-induced
acute
lung
injury
(ALI).
Akkermansia
muciniphila
(A.
muciniphila)
maintains
the
intestinal
barrier
function
and
regulates
balance
of
reduced
glutathione/oxidized
glutathione.
However,
it
may
be
useful
as
a
treatment
strategy
for
LPS-induced
injury.
Our
study
aimed
explore
whether
A.
could
improve
by
affecting
gut
microbiota.
The
administration
effectively
attenuated
tissue
damage
significantly
decreased
oxidative
stress
inflammatory
reaction
induced
LPS,
with
lower
levels
myeloperoxidase
(MDA),
enhanced
superoxide
dismutase
(SOD)
activity,
pro-inflammatory
cytokine
levels,
macrophage
neutrophil
infiltration.
Moreover,
maintained
function,
reshaped
disordered
microbial
community,
promoted
secretion
short-chain
fatty
acids
(SCFAs).
downregulated
expression
TLR2,
MyD88
NF-kappa
B
(P
<
0.05).
Butyrate
supplementation
demonstrated
significant
improvement
in
response
0.05)
mitigation
histopathological
mice
ALI,
thereby
restoring
butyric
acid
concentration.
In
conclusion,
our
findings
indicate
that
inhibits
accumulation
cytokines
attenuates
activation
TLR2/Myd88/NF-κB
pathway
due
exerting
anti-inflammatory
effects
through
butyrate.
This
provides
an
experimental
foundation
potential
application
butyrate
prevention
ALI.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: Feb. 6, 2024
Akkermansia
muciniphila
is
a
gram-negative
bacterium
that
colonizes
the
human
gut,
making
up
3–5%
of
microbiome.
A.
promising
next-generation
probiotic
with
clinical
application
prospects.
Emerging
studies
have
reported
various
beneficial
effects
including
anti-cancer,
delaying
aging,
reducing
inflammation,
improving
immune
function,
regulating
nervous
system
whereas
knowledge
on
its
roles
and
mechanism
in
infectious
disease
currently
unclear.
In
this
review,
we
summarized
basic
characteristics,
genome
phenotype
diversity,
influence
derived
components
diseases,
such
as
sepsis,
virus
infection,
enteric
periodontitis
foodborne
pathogen
induced
infections.
We
also
provided
updates
mechanisms
how
protects
intestinal
barrier
integrity
modulate
host
response.
summary,
believe
therapeutic
may
be
applied
for
treatment
variety
diseases.
Biomedicine & Pharmacotherapy,
Journal Year:
2024,
Volume and Issue:
173, P. 116416 - 116416
Published: March 11, 2024
Colorectal
cancer
(CRC)
is
the
second
most
cancer-related
death
worldwide.
In
recent
years,
probiotics
have
been
used
to
reduce
potential
risks
of
CRC
and
tumors
with
various
mechanisms.
Different
bacteria
suggested
play
different
roles
in
progression,
prevention,
or
treatment
CRC.
Akkermansia
muciniphila
considered
a
next-generation
probiotic
for
preventing
treating
some
diseases.
Therefore,
this
review
article,
we
aimed
describe
discuss
mechanisms
A.
as
an
intestinal
microbiota
Some
studies
that
abundance
was
higher
increased
patients
compared
healthy
individuals.
However,
decreased
associated
severe
symptoms
CRC,
indicating
did
not
role
development
addition,
administration
elevates
gene
expression
proliferation-associated
molecules
such
S100A9,
Dbf4,
Snrpd1,
markers
cell
proliferation.
other
inflammation
tumorigenesis
intestine
might
promoted
by
muciniphila.
Overall,
inhibition
still
unclear
controversial.
Various
methods
bacterial
supplementation,
viability,
number,
abundance,
could
all
influence
colonization
effect
progression.
mucinipila
has
revealed
modulate
therapeutic
immune
checkpoint
inhibitors.
Preliminary
human
data
propose
oral
consumption
safe,
but
its
efficacy
needs
be
confirmed
more
clinical
studies.
Microbiome,
Journal Year:
2024,
Volume and Issue:
12(1)
Published: July 1, 2024
Abstract
Background
Fecal
microbiota
transplantation
(FMT)
and
fecal
virome
(FVT,
sterile
filtrated
donor
feces)
have
been
effective
in
treating
recurrent
Clostridioides
difficile
infections,
possibly
through
bacteriophage-mediated
modulation
of
the
gut
microbiome.
However,
challenges
like
variability,
costly
screening,
coupled
with
concerns
over
pathogen
transfer
(incl.
eukaryotic
viruses)
FMT
or
FVT
hinder
their
wider
clinical
application
less
acute
diseases.
Methods
To
overcome
these
challenges,
we
developed
methods
to
broaden
FVT’s
while
maintaining
efficacy
increasing
safety.
Specifically,
employed
following
approaches:
(1)
chemostat-fermentation
reproduce
bacteriophage
component
remove
viruses
(FVT-ChP),
(2)
solvent-detergent
treatment
inactivate
enveloped
(FVT-SDT),
(3)
pyronin-Y
inhibit
RNA
virus
replication
(FVT-PyT).
We
assessed
processed
FVTs
a
C.
infection
mouse
model
compared
them
untreated
(FVT-UnT),
FMT,
saline.
Results
FVT-SDT,
FVT-UnT,
FVT-ChP
reduced
incidence
mice
reaching
humane
endpoint
(0/8,
2/7,
3/8,
respectively)
FVT-PyT,
saline
(5/8,
7/8,
5/7,
significantly
load
colonizing
cells
associated
toxin
A/B
levels.
There
was
potential
elimination
colonization,
seven
out
eight
treated
FVT-SDT
testing
negative
qPCR.
In
contrast,
all
other
treatments
exhibited
continued
presence
.
Moreover,
results
were
supported
by
changes
microbiome
profiles,
cecal
cytokine
levels,
histopathological
findings.
Assessment
viral
engraftment
FMT/FVT
host-phage
correlations
analysis
suggested
that
phages
likely
an
important
contributing
factor
efficacy.
Conclusions
This
proof-of-concept
study
shows
specific
modifications
hold
promise
addressing
related
variability
risks.
Two
strategies
lead
limiting
colonization
mice,
solvent/detergent
chemostat
propagation
emerging
as
promising
approaches.
Gut Microbes,
Journal Year:
2024,
Volume and Issue:
16(1)
Published: Jan. 2, 2024
Hyperbaric
oxygen
(HBO)
therapy
is
a
well-established
method
for
improving
tissue
oxygenation
and
typically
used
the
treatment
of
various
inflammatory
conditions,
including
infectious
diseases.
However,
its
effect
on
intestinal
mucosa,
microenvironment
known
to
be
physiologically
hypoxic,
remains
unclear.
Here,
we
demonstrated
that
daily
with
hyperbaric
affects
gut
microbiome
composition,
worsening
antibiotic-induced
dysbiosis.
Accordingly,
HBO-treated
mice
were
more
susceptible
Clostridioides
difficile
infection
(CDI),
an
enteric
pathogen
highly
associated
colitis.
These
observations
closely
linked
decline
in
level
microbiota-derived
short-chain
fatty
acids
(SCFAs).
Butyrate,
SCFA
produced
primarily
by
anaerobic
microbial
species,
mitigated
HBO-induced
susceptibility
CDI
increased
epithelial
barrier
integrity
group
3
innate
lymphoid
cell
(ILC3)
responses.
Mice
displaying
tissue-specific
deletion
HIF-1
RORγt-positive
cells
exhibited
no
protective
butyrate
during
CDI.
In
contrast,
reinforcement
signaling
through
conditional
VHL
disease
outcome,
even
after
HBO
therapy.
Taken
together,
conclude
induces
dysbiosis
impairs
production
SCFAs
affecting
HIF-1α-IL-22
axis
ILC3
response
subsequent
C.
infection.
Gut Microbes,
Journal Year:
2023,
Volume and Issue:
15(1)
Published: July 10, 2023
A
high-fat
(HF)
diet
reduces
resistance
to
the
foodborne
pathogen
Listeria
monocytogenes.
We
demonstrate
that
short-term
gavage
with
A.
muciniphila
increases
oral
and
systemic
L.
monocytogenes
infection
in
mice
fed
a
HF
diet.
reduced
inflammation
gut
liver
of
prior
inflammatory
cell
infiltration
ileum
levels
similar
low-fat
(LF)
Akkermansia
administration
had
minimal
impacts
upon
microbiota
microbial
metabolites
did
not
affect
individual
taxa
or
impact
Bacteroidetes
Firmicutes
ratio.
In
summary,
increased
by
moderating
immune/physiological
effects
through
specific
interaction
between
host
gut.
