Biology,
Journal Year:
2024,
Volume and Issue:
13(6), P. 372 - 372
Published: May 23, 2024
The
gut
microbiota
constitutes
a
complex
ecosystem
that
has
an
important
impact
on
host
health.
In
this
study,
genetically
engineered
zebrafish
with
inducible
nitric
oxide
synthase
(iNOS
or
NOS2)
knockout
were
used
as
model
to
investigate
the
effects
of
nos2a/nos2b
gene
single
and
nos2
double
intestinal
microbiome
composition
function.
Extensive
16S
rRNA
sequencing
revealed
substantial
changes
in
microbial
diversity
specific
taxonomic
abundances,
yet
it
did
not
affect
functional
structure
tissues.
Notably,
iNOS-deficient
demonstrated
decrease
Vibrio
species
increase
Aeromonas
species,
more
pronounced
observed
knockouts.
Further
transcriptomic
analysis
iNOS
indicated
significant
alterations
immune-related
metabolic
pathways,
including
complement
PPAR
signaling
pathways.
These
findings
underscore
crucial
interplay
between
genetics
microbiota,
indicating
plays
key
role
modulating
ecology,
immune
system,
responses.
Antioxidants,
Journal Year:
2023,
Volume and Issue:
12(1), P. 192 - 192
Published: Jan. 13, 2023
Oxidative
stress
is
a
phenomenon
caused
by
an
imbalance
between
the
production
and
accumulation
of
reactive
oxygen
species
in
cells
tissues
that
eventually
leads
to
various
diseases.
Here,
we
investigated
antioxidant
effects
extract
from
Sonchus
brachyotus
DC.
(SBE)
based
on
0.2%
oxazolone-induced
intestinal
oxidative
model
zebrafish.
Compared
group,
treatment
group
alleviated
tissue
damage
reduced
contents
malondialdehyde,
species,
IL-1β,
TNF-α
then
increased
superoxide
dismutase,
glutathione
peroxidase,
IL-10.
The
16s
rDNA
gene
sequencing
findings
demonstrated
SBE
could
increase
relative
abundance
Fusobacteriota,
Actinobacteriota,
Firmicutes
decrease
Proteobacteria.
Based
correlation
analysis
biomarkers
flora,
found
trends
were
significantly
correlated
with
microorganisms,
especially
at
genus
level.
correlations
MDA,
negative
Shewanella,
while
SOD,
GSH-Px,
IL-10
positive
Cetobacterium,
Gemmobacter,
Flavobacterium.
Consequently,
concluded
effect
was
realized
through
interaction
gut
microbiota.
Frontiers in Physiology,
Journal Year:
2023,
Volume and Issue:
14
Published: June 1, 2023
Zebrafish
(
Danio
rerio
)
have
emerged
as
a
powerful
model
to
study
the
gut
microbiome
in
context
of
human
conditions,
including
hypertension,
cardiovascular
disease,
neurological
disorders,
and
immune
dysfunction.
Here,
we
highlight
zebrafish
tool
bridge
gap
knowledge
linking
physiological
homeostasis
cardiovascular,
neural,
systems,
both
independently
an
integrated
axis.
Drawing
on
studies
date,
discuss
challenges
microbiota
transplant
techniques
gnotobiotic
husbandry
practices.
We
present
advantages
current
limitations
research
use
identification
microbial
enterotypes
health
disease.
also
versatility
further
explore
function
conditions
relevant
dysbiosis
reveal
novel
therapeutic
targets.
Dietary
protein
absorption
in
neonatal
mammals
and
fishes
relies
on
the
function
of
a
specialized
conserved
population
highly
absorptive
lysosome-rich
enterocytes
(LREs).
The
gut
microbiome
has
been
shown
to
enhance
nutrients,
such
as
lipids,
by
intestinal
epithelial
cells.
However,
whether
is
also
affected
poorly
understood.
Here,
we
investigate
connections
between
microbes
zebrafish
gut.
Using
live
microscopy-based
quantitative
assays,
find
that
slow
pace
uptake
degradation
LREs.
While
do
not
affect
number
absorbing
LRE
cells,
lower
expression
endocytic
digestion
machinery
transgene-assisted
cell
isolation
single
RNA-sequencing,
characterize
all
cells
take
up
dietary
protein.
We
bacteria-sensing
metabolic
pathways
LREs,
some
secretory
types
share
components
with
custom-formulated
diets,
investigated
influence
diet
activity
microbiome.
Impaired
along
protein-deficient
diet,
alters
microbial
community
leads
an
increased
abundance
bacterial
genera
have
capacity
reduce
Together,
these
results
reveal
diet-dependent
reciprocal
interactions
LREs
regulate
absorption.
PeerJ,
Journal Year:
2024,
Volume and Issue:
12, P. e17051 - e17051
Published: March 27, 2024
Fishes
are
hosts
for
many
microorganisms
that
provide
them
with
beneficial
effects
on
growth,
immune
system
development,
nutrition
and
protection
against
pathogens.
In
order
to
avoid
spreading
of
infectious
diseases
in
aquaculture,
prevention
includes
vaccinations
routine
disinfection
eggs
equipment,
while
curative
treatments
consist
the
administration
antibiotics.
Vaccination
processes
can
stress
fish
require
substantial
farmer’s
investment.
Additionally,
antibiotics
not
specific,
they
may
be
effective
short
term,
have
major
drawbacks
long
term.
Indeed,
eliminate
bacteria
which
useful
host
promote
raising
antibiotic
resistance
beneficial,
commensal
but
also
pathogenic
bacterial
strains.
Numerous
publications
highlight
importance
plays
diversified
microbial
community
colonizing
(i.e.,
microbiota)
health
ultimately
survival
their
host.
This
review
targets
current
knowledge
bidirectional
communication
between
microbiota
during
development.
It
explores
extent
this
mutualistic
relationship:
one
hand,
effect
microbes
exert
ontogeny
fishes,
other
impact
critical
steps
development
recruitment
succession
throughout
life.
We
will
first
describe
its
gene
expression
fishes.
Secondly,
plurality
microbiotas
(depending
organism,
organ,
stage)
reviewed.
Then,
a
description
constant
interactions
fish’s
life
stages
discussed.
Healthy
allow
maturation
modulation
inflammation,
both
contribute
homeostasis.
Thus,
equilibrium
is
closely
linked
stability
examples
from
several
species
more
extensively
mechanisms
occurring
zebrafish
model
because
much
finely
described
species,
thanks
existing
mutants
precise
investigations.
conclude
how
conceptual
framework
associated
research
benefit
considering
relations
maturation.
More
precisely,
active
tolerance
earliest
enables
sustainable
establishment
complex
healthy
adult
Establishing
balanced
host-microbiota
interaction
avoids
triggering
deleterious
maintains
immunological
microbiological
