Developmental Dynamics of the Gut Virome in Tibetan Pigs at High Altitude: A Metagenomic Perspective across Age Groups DOI Creative Commons
Runbo Luo,

Aohan Guan,

Bin Ma

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(4), P. 606 - 606

Published: April 14, 2024

Tibetan pig is a geographically isolated breed that inhabits high-altitude areas of the Qinghai–Tibetan plateau. At present, there limited research on viral diseases in pigs. This study provides novel metagenomic exploration gut virome pigs (altitude ≈ 3000 m) across three critical developmental stages, including lactation, nursery, and fattening. The composition communities intestine, with dominant presence Microviridae phages observed all stages development, combination previous literature, suggest it may be associated geographical locations high altitude. Functional annotation operational taxonomic units (vOTUs) highlights that, among constantly increasing vOTUs groups, adaptability viruses to environmental stressors such as salt heat indicates an evolutionary response conditions. It shows lactation group has more abundant auxiliary metabolic genes (vAMGs) than nursery fattening groups. During this leaves only DNMT1 at level. which contributing factor promoting health. found preferentially adopt lytic lifestyles stages. These findings not elucidate dynamic interplay between host offering insights into ecology their adaptation environments, but also provide theoretical basis for further studies production epidemic prevention under extreme

Language: Английский

Influence of selected dosages of plastic microparticles on the porcine fecal microbiome DOI Creative Commons
Ismena Gałęcka, Andrzej Rychlik, Jarosław Całka

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 8, 2025

Abstract Studies conducted so far have shown that nano- and microplastic may disturb the intestinal microenvironment by interacting with epithelium gut microbiota. Depending on research model used, effect microbiome is different—an increase or decrease in selected taxa resulting development of dysbiosis. Dysbiosis be associated inflammation, mental disorders diabetes. The aim study was to analyze 15 gilts divided into 3 groups (n = 5; control group, receiving micropartices at a dose 0.1 g/day (LD) 1 (HD)). Feaces were collected before after 28 days exposure PET microplastics. analysis performed using next-generation sequencing. Alpha beta diversity indices compared, showing, repetition affected only abundance LD groups, but not HD group. relationships between number reads phylum, genus species level calculated statistical methods ( r -Pearson correlation, generalized regression model, variance). revealed, populations Family XIII AD3011 group , Coprococcus V9D2013 UCG-010 Sphaerochaeta increased increasing MP-PET dose. above-mentioned are mainly responsible for production short-chain fatty acids (SCFA). It assumed, SCFA one mechanisms involved response oral MP-PET.

Language: Английский

Citations

1
Key Taxa of the Gut Microbiome Associated with the Relationship Between Environmental Sensitivity and Inflammation-Related Biomarkers DOI Creative Commons
Satoshi Takasugi, Shuhei Iimura,

Miyabi Yasuda

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 185 - 185

Published: Jan. 16, 2025

Individual differences in environmental sensitivity are linked to stress-related psychiatric symptoms. In previous research, we found that high can be a risk factor for increased inflammation and gut permeability, particularly when microbiome diversity is low. However, the specific bacterial taxa involved this interaction remain unclear. As preliminary study, research aimed identify key associated with relationship. Environmental sensitivity, composition, permeability (lipopolysaccharide-binding protein, LBP), (C-reactive CRP) biomarkers were evaluated 88 participants. The between relative abundance of family Marinifilaceae (genus Butyricimonas) was predictor CRP levels. Similarly, Barnesiellaceae Coprobacter), Akkermansiaceae Akkermansia), genus Family XIII AD3011 group, GCA-900066225, or Ruminiclostridium 1 predicted LBP Individuals exhibited elevated levels these Conversely, highly sensitive individuals had lower high. This study suggests serve as one protective factors against sensitivity. Further in-depth studies needed confirm associations understand underlying mechanisms.

Language: Английский

Citations

1
The Effect of an Early-Life Lactiplantibacillus plantarum LPJZ-658 Intervention on Performance and Gut Microbiota in Suckling Piglets DOI Creative Commons
Zhuo Chen, Ziqi Liu, Chen Chen

et al.

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(2), P. 41 - 41

Published: Feb. 7, 2025

This study aimed to investigate the effects of early-life supplementation with Lactiplantibacillus plantarum LPJZ-658 on growth performance and gut microbiota newborn piglets. Twelve one-day-old suckling piglets were randomly divided into two groups: control group (C) was orally administered saline (LP) gavaged 1.0 × 1010 cfu LPJZ-658. The carried out once daily for 28 days. Fresh fecal samples collected at 7 days, respectively. composition (16S rRNA gene amplicon sequencing) its predicted functions (PICRUSt2) analyzed. body weight average gain significantly increased in LP group. Statistically significant differences observed bacterial diversity microbial community between C groups. predominant phylum changed from Firmicutes, Bacteroidetes, Proteobacteria day Spirochaetota both We found that suppressed a decrease relative abundance Bacteroidota At genus level, reduced pathogenic genera such as Clostridium sensu stricto 1, beneficial Ruminococcus, Christensenellaceae_R-7_group, Turicibacter, UCG-002. KEGG metabolic pathway analysis showed may improve amino acid metabolism by regulating intestinal In summary, interventions modulation microbiota.

Language: Английский

Citations

1
Association of gut microbiota and SCFAs with finishing weight of Diannan small ear pigs DOI Creative Commons

Qun Lan,

Yuju Lian,

Peiya Peng

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 27, 2023

Finishing weight is a key economic trait in the domestic pig industry. Evidence has linked gut microbiota and SCFAs to health production performance pigs. Nevertheless, for Diannan small ear (DSE) pigs, specific breed China, potential effect of on their finishing remains unclear. Herein, based data 16S ribosomal RNA gene metagenomic sequencing analysis, we found that 13 OTUs could be biomarkers 19 microbial species were associated with weight. Among these, carbohydrate-decomposing bacteria families Streptococcaceae, Lactobacillaceae, Prevotellaceae positively related weight, whereas taxa intestinal inflammation damage exhibited opposite effects. In addition, interactions these first time. Gut functional annotation analysis indicated CAZymes, such as glucosidase glucanase significantly affect given roles increasing nutrient absorption efficiency. Kyoto Encyclopedia Genes Genomes (KEGG) Orthologies (KOs) KEGG pathways glycolysis/gluconeogenesis, phosphotransferase system (PTS), secondary bile acid biosynthesis, ABC transporters, sulfur metabolism, one carbon pool by folate act factors regulating Additionally, SCFA levels, especially acetate butyrate, had pivotal impacts weight-associated Prevotella sp. RS2, Ruminococcus AF31-14BH Lactobacillus pontis showed positive associations butyrate concentration, Paraprevotella xylaniphila Bacteroides OF04-15BH level. Taken together, our study provides essential knowledge manipulating microbiomes improve The underlying mechanisms how microbiome modulate pigs' required further elucidation.

Language: Английский

Citations

19
Panax notoginseng stems and leaves affect microbial community and function in cecum of duzang pigs DOI Creative Commons

Lanlan Yi,

Junhong Zhu, Qiuyan Li

et al.

Translational Animal Science, Journal Year: 2024, Volume and Issue: 8

Published: Jan. 1, 2024

is a Chinese medicine with long history in which stems and leaves are the wastes of processing

Language: Английский

Citations

5
Comparative Analysis of Intestinal Inflammation and Microbiota Dysbiosis of LPS-Challenged Piglets between Different Breeds DOI Creative Commons
Chao Li, Yanping Wang, Xueyan Zhao

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(5), P. 665 - 665

Published: Feb. 20, 2024

Post-weaning diarrhea is common in piglets, causing huge economic losses worldwide. Associations between LPS challenge, intestinal inflammation, and microbiota have been reported Duroc × Landrace Yorkshire (DLY) crossbred pigs. However, the effects of challenge other breeds remain unclear. In current study, we performed a comprehensive comparative analysis on jejunal mucosal morphology, microbial composition, serum indexes two pig breeds: DLY Heigai, an indigenous Chinese breed. The results showed that caused considerable damage to enhanced levels inflammatory cytokines permeability index, lowered antioxidant capacity index. also changed composition structure jejunum, significantly increased abundances Escherichia-Shigella pigs, decreased those Gemella Saccharimonadales Heigai Furthermore, triggered functional changes energy metabolism activities related stress response bacterial community, alleviating This study revealed pigs had weaker immune than identified several genera breed-specific phenotypes including Gemella, Saccharimonadales, Clostridia_UCG_014, Terrisporobacter, Dielma. Our collective findings uncovered differences inflammation dysbiosis induced by providing theoretical basis for unraveling mechanism swine proposing candidates involved resistance piglets.

Language: Английский

Citations

5
Mendelian randomization distinguished the changes in gut microbiota caused by IBD from those that lead to IBD DOI Creative Commons
Fan Li, Xiaoping Han,

Tongyu Tang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 7, 2024

Abstract Observational studies have reported that dysbiosis of gut microbiota may be a contributing factor in the development IBD. However, causal relationship between and IBD remains unclear due to coexistence patients. The aim this study is investigate reverse relationships inflammatory bowel disease (IBD).We aimed explore using two-sample Mendelian randomization (MR) approach. Specifically, genetic variants associated with were selected from International Genetics Consortium (IIBDGC) trans-ancestry genome-wide association (GWAS), while data related obtained MiBioGen consortium. We employed various MR methods, including inverse variance-weighted (IVW), MR-Egger regression, weighted median, mode, MR-RAPS. Sensitivity analyses intercept test, Cochran's Q leave-one-out test conducted for quality control.As results, Bifidobacteriaceae, Bifidobacterium, Lactobacillaceae, Lachnospiraceae UCG008 Paraprevotella reduce risk CD. Family XIII AD3011 reduces CD UC. Clostridiales vadin BB60 group UCG010 genus, Oxalobacteraceae, Oxalobacter Intestinimonas promotes development. Paraprevotella, Eubacterium ruminantium Prevotellaceae genus UC coprostanoligenes, Ruminococcaceae UCG005 development.Based on intercept, there no evidence potential heterogeneity or pleiotropy bias our findings. In conclusion, suggests use novel probiotics adjunctive therapy present new avenue future treatment.

Language: Английский

Citations

4
Identification of Selection Signatures and Candidate Genes Related to Environmental Adaptation and Economic Traits in Tibetan Pigs DOI Creative Commons
Pengliang Liu, Yan Liang, Li Li

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(4), P. 654 - 654

Published: Feb. 19, 2024

Tibetan pigs are indigenous to the Qinghai–Tibet Plateau and have been subject of extensive genomic research primarily focused on their adaptation high altitudes. However, genetic modifications associated with response low-altitude acclimation not thoroughly explored. To investigate basis underlying pigs, we generated analyzed genotyping data that inhabit high-altitude regions (average altitude 4000 m) inhabited nearby 500 for approximately 20 generations. We found highland lowland distinguishable genotype phenotype variations. identified 46 126 potentially selected SNPs 29 56 candidate genes in respectively. Candidate were involved immune (NFYC STAT1) radiation (NABP1), whereas related reproduction (ESR2, DMRTA1, ZNF366), growth development (NTRK3, FGF18, MAP1B), blood pressure regulation (CARTPT). These findings will help understand mechanisms environmental offer valuable information into improvement pertaining economic traits.

Language: Английский

Citations

4
Metagenome-assembled microbial genomes (n = 3,448) of the oral microbiomes of Tibetan and Duroc pigs DOI Creative Commons

Hong Hu,

Ying Huang,

Fuyan Yang

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: Jan. 24, 2025

Compared with leaner breeds, local Chinese pig breeds have distinct intestinal microbial, as determined by metagenomic techniques, and the interactions between oral microorganisms their hosts are also gradually being clarified. However, high host genome content means that few metagenome-based microbiomes been reported. Here, we combined dilution-based sequencing binning approaches to extract microbial genomes from of Tibetan Duroc pigs. The contamination rates were reduced 13.64%, a quarter normal level (65.25% on average). Medium–high-quality metagenome-assembled (MAGs; n = 3,448) spanning nine phyla retrieved 70.79% novel species. Of nonredundant MAGs, only 13.37% shared, revealing strong disparities diversity was greater than pig. We present first large-scale dilute-based data microbiome, which should facilitate further investigation functions in

Language: Английский

Citations

0
Fecal microbiota transplantation modulates jejunal host-microbiota interface in weanling piglets DOI Creative Commons
Shankumar Mooyottu, Muhammed Shafeekh Muyyarikkandy,

Farnaz Yousefi

et al.

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: Feb. 7, 2025

Language: Английский

Citations

0