Sesamin Alleviates Allergen-Induced Diarrhea by Restoring Gut Microbiota Composition and Intestinal Barrier Function

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Food allergens are the key triggers of allergic diarrhea, causing damage to immune-rich ileum. This weakens mucosal barrier and tight junctions, increases intestinal permeability, exacerbates allergen exposure, thereby worsening condition. Sesamin, a natural lignan isolated from sesame seed, has shown potential in regulating immune responses, but its effects on health remain unclear. In this study, we constructed an ovalbumin (OVA)-induced diarrhea mouse model, which demonstrated increased mast cell degranulation, reduced junction integrity, impaired function. Pro-inflammatory cytokines were significantly ileum, along with unbalanced cluster differentiation 4 (CD4

Language: Английский

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Recent progress in plant-derived polysaccharides with prebiotic potential for intestinal health by targeting gut microbiota: a review

Critical Reviews in Food Science and Nutrition, Journal Year: 2023, Volume and Issue: 64(33), P. 12242 - 12271

Published: Aug. 31, 2023

Natural products of plant origin are high interest and widely used, especially in the food industry, due to their low toxicity wide range bioactive properties. Compared other components, safety polysaccharides has been generally recognized. As dietary fibers, plant-derived mostly degraded intestine by polysaccharide-degrading enzymes secreted gut microbiota, have potential prebiotic activity both non-disease disease states, which should not be overlooked, terms involvement treatment intestinal diseases promotion health. This review elucidates regulatory effects on microbiota summarizes mechanisms involved targeting for diseases. Further, structure-activity relationships between different structural types occurrence further explored. Finally, practical applications production packaging summarized discussed, providing important references expanding application industry or developing functional supplements.

Language: Английский

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Lactiplantibacillus plantarum P9 for chronic diarrhea in young adults: a large double-blind, randomized, placebo-controlled trial

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 9, 2024

Current treatments for chronic diarrhea have limited efficacy and several side effects. Probiotics the potential to alleviate symptoms of diarrhea. This randomized, double-blind, placebo-controlled trial evaluates effects administering probiotic Lactiplantibacillus plantarum P9 (P9) strain in young adults with (Clinical Trial Registration Number: ChiCTR2000038410). The intervention period lasts 28 days, followed by a 14-day post-intervention period. Participants are randomized into (n = 93) placebo 96) groups, 170 individuals completing double-blind phase 85 per group). primary endpoint is symptom severity score. Both intention-to-treat 189) per-protocol 170) analyses reveal modest yet statistically significant reduction compared group (20.0%, P 0.050; 21.4%, 0.048, respectively). In conclusion, results this study support use probiotics managing adults. However, lack blood parameter assessment short represent limitations study.

Language: Английский

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Comparison of differentially expressed genes in longissimus dorsi muscle of Diannan small ears, Wujin and landrace pigs using RNA-seq

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 10

Published: Jan. 5, 2024

Introduction Pig growth is an important economic trait that involves the co-regulation of multiple genes and related signaling pathways. High-throughput sequencing has become a powerful technology for establishing transcriptome profiles can be used to screen genome-wide differentially expressed (DEGs). In order elucidate molecular mechanism underlying muscle growth, this study adopted RNA (RNA-seq) identify compare DEGs at genetic level in longissimus dorsi (LDM) between two indigenous Chinese pig breeds (Diannan small ears [DSE] Wujin [WJ]) one introduced breed (Landrace [LP]). Methods Animals under were from (DSE pig, n = 3; WJ 3) (LP, expression levels LDM. Then, functional annotation, Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia Genes Genomes (KEGG) pathway Protein–Protein Interaction (PPI) network analysis performed on these DEGs. Protein-Protein Results The results revealed DSE, WJ, LP libraries, more than 66, 65, 71 million clean reads generated by sequencing, respectively. A total 11,213 identified LDM tissue breeds, which 7,127 co-expressed three samples. total, 441 339 DSE vs. study, with 254, 193 up-regulated 187, down-regulated compared LP. GO KEGG showed are significantly contractile fiber, sarcolemma, dystrophin-associated glycoprotein complex, myofibril, myosin II Glycolysis/Gluconeogenesis, Propanoate metabolism, Pyruvate etc. combination annotation DEGs, key such as ENO3 JUN PPI analysis. Discussion conclusion, present including DES, FLNC, PSMD1, PSMD6, PSME4, PSMB4, RPL11, RPL13A, ROS23, RPS29, MYH1, MYL9, MYL12B, TPM1, TPM4, ENO3, PGK1, PKM2, GPI , unannotated new gene ENSSSCG00000020769 pathways influence difference could provide theoretical basis improving traits future.

Language: Английский

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Beneficial Effects of Synbiotics on the Gut Microbiome in Individuals with Low Fiber Intake: Secondary Analysis of a Double-Blind, Randomized Controlled Trial

Nutrients, Journal Year: 2024, Volume and Issue: 16(13), P. 2082 - 2082

Published: June 29, 2024

Insufficient dietary fiber intake can negatively affect the intestinal microbiome and, over time, may result in gut dysbiosis, thus potentially harming overall health. This randomized controlled trial aimed to improve of individuals with low (<25 g/day) during a 7-week synbiotic intervention. The metabolically healthy male participants (n = 117, 32 ± 10 y, BMI 25.66 3.1 kg/m2) were divided into two groups: one receiving supplement (Biotic Junior, MensSana AG, Forchtenberg, Germany) and other placebo, without altering their habits or physical activity. These groups further stratified by group (LFG) high (HFG). Stool samples for analysis collected before after Statistical was performed using linear mixed effects partial least squares models. At baseline, microbiomes LFG HFG partially separated. After seven weeks intervention, abundance SCFA-producing microbes significantly increased LFG, which is known health; however, this effect less pronounced HFG. Beneficial on be achieved synbiotics, demonstrating importance personalized synbiotics.

Language: Английский

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Intrauterine growth restriction, defined by an elevated brain-to-liver weight ratio, affects faecal microbiota composition and, to a lesser extent, plasma metabolome profile at different ages in pigs

Animal Microbiome, Journal Year: 2025, Volume and Issue: 7(1)

Published: Feb. 19, 2025

Intrauterine growth restriction (IUGR) affects up to 30% of piglets in a litter. Piglets exposed IUGR prioritize brain development during gestation, resulting higher brain-to-liver weight ratio (BrW/LW) at birth. is associated with increased mortality, compromised metabolism, and gut health. However, the dynamic metabolic microbial shifts IUGR-affected pigs remain poorly understood. This study aimed investigate longitudinal effects IUGR, defined by high BrW/LW, on composition faecal microbiota plasma metabolome from birth slaughter. One day (± 1) after birth, computed tomography was performed each piglet assess their liver weights. The highest (IUGR = 12) lowest (NORM BrW/LW were selected collect faeces blood lactation (day 16 ± 0.6, T1) end starter period 63 8.6, T2) beginning 119 11.4, T3) finisher 162 14.3, T4). Faecal Alpha diversity remained unaffected across all time points. Beta influenced T1 (P 0.002), T2 0.08), T3 0.03). Specifically, displayed abundances Clostridium sensu stricto 1 (Padj 0.03) Romboutsia 0.05) T1, Prevotellaceae NK3B31 group 0.02), Rikenellaceae RC9 0.03), Alloprevotella T2, p-2534-18B5 T3. Conversely, NORM exhibited Ruminococcus 0.01) HT002 Prevotella_9 < 0.001) None metabolites showed significant differences between pigs. asparagine lower compared 0.05). These findings show that uterus has impact pigs, period, but minimally profile.

Language: Английский

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Dietary Trends, Microbiome, and Cardiovascular Health: A Narrative Review

Heart and Mind, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Abstract Cardiovascular (CV) disease (CVD) remains a leading cause of morbidity and mortality worldwide, with growing evidence highlighting the role diet gut microbiome in cardiovascular health (CVH). This review synthesizes current knowledge on interactions between dietary patterns, microbiome, CV outcomes. High-fiber diets, such as Mediterranean Dietary Approaches to Stop Hypertension, promote beneficial microbial species, enhancing production short-chain fatty acids that exhibit anti-inflammatory cardioprotective effects. Conversely, Western diets high fat low fiber are associated dysbiosis elevated trimethylamine-N-oxide (TMAO) levels, metabolite linked increased atherosclerosis CVD risk. TMAO has been shown amplify systemic inflammation by upregulating pro-inflammatory cytokines, interleukin-6 tumor necrosis factor-alpha, exacerbating damage. explores how composition influences metabolic immune functions, contributing endothelial dysfunction, which critical progression. Personalized nutrition, informed profiling, represents promising avenue for optimizing CVH. Emerging therapeutic strategies, including probiotics, prebiotics, fecal microbiota transplantation, also offer potential modulating improve

Language: Английский

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Improvement of antioxidant capacity and gut microbiota balance in adult zebrafish (Danio rerio) by addition of fermented distillers’ dried grains with solubles (DDGS)

Fish Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 51(3)

Published: May 7, 2025

Language: Английский

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Different Diet Energy Levels Alter Body Condition, Glucolipid Metabolism, Fecal Microbiota and Metabolites in Adult Beagle Dogs

Metabolites, Journal Year: 2023, Volume and Issue: 13(4), P. 554 - 554

Published: April 13, 2023

Diet energy is a key component of pet food, but it usually ignored during food development and owners also have limited knowledge its importance. This study aimed to explore the effect diet on body condition, glucolipid metabolism, fecal microbiota metabolites adult beagles analyze relation between host gut microbiota. Eighteen healthy neutered male were selected randomly divided into three groups. Diets formulated with metabolizable (ME) levels: low-energy (Le) group consumed 13.88 MJ/kg ME; medium-energy (Me) 15.04 high-energy (He) 17.05 ME. Moreover, protein content all these diets was 29%. The experiment lasted 10 weeks, two-week acclimation period an eight-week test phase. Body weight, condition score (BCS), muscle (MCS) fat index (BFI) decreased in Le group, changes factors significantly higher than other groups (p < 0.05). serum glucose lipid levels He changed over time 0.05), those Me stable > pH at end trial 0.05) we found that profiles short-chain fatty acids (SCFAs) bile (BAs) greatly, especially secondary BAs As SCFAs are microbiota, measured. Fecal 16S rRNA gene sequencing had α-diversity indices notably probiotics, such as Faecalibacterium prausnitzii, Bacteroides plebeius Blautia producta diet-host-fecal interactions determined by network analysis, may help determine best physical dogs, assisting development. Overall, feeding dogs low- or harmful for glucostasis promoted relative abundance pathogenic bacteria gut, while maintained ideal condition. We concluded fed extended become lean lose mass, low 29% not supply enough losing weight.

Language: Английский

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Microbial signatures and enterotype clusters in fattening pigs: implications for nitrogen utilization efficiency

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 10, 2024

As global demand for pork continues to rise, strategies enhance nitrogen utilization efficiency (NUE) in pig farming have become vital environmental sustainability. This study explored the relationship between fecal microbiota, their metabolites, and NUE crossbreed fattening pigs with a defined family structure. Pigs were kept under standardized conditions fed two-phase feeding regime. In each phase, one sample was collected from pig. DNA extracted total of 892 samples subjected target amplicon sequencing. The results indicated an influence sire, sampling period (SP), sex on microbiota.

Language: Английский

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