Seeds Act as Vectors for Antibiotic Resistance Gene Dissemination in a Soil-Plant Continuum

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(50), P. 21358 - 21369

Published: Dec. 11, 2023

Though the evidence for antibiotic resistance spread via plant microbiome is mounting, studies regarding resistome in seed, a reproductive organ and important food resource, are still their infancy. This study investigated effects of long-term organic fertilization on seed bacterial endophytes, resistome, intergenerational transfer microcosm. A total 99 genes (ARGs) 26 mobile genetic elements (MGEs) were detected by high-throughput quantitative PCR. The amount fertilizer applied was positively correlated to number relative abundance seed-associated ARGs MGEs. Moreover, transmission from rhizosphere mainly mediated shared bacteria Notably, progeny seedlings derived seeds harboring abundant found have higher ARGs. Using structural equation models, we further revealed that MGEs key factors affecting rhizosphere, implying potential reservoir soil. highlights overlooked role endophytes dissemination soil-plant continuum, more attention should be paid as vectors within "One-Health" framework.

Language: Английский

---

Erythromycin fermentation residue exposure induces a short-term wave of antibiotic resistance in a soil-lettuce system

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 902, P. 166081 - 166081

Published: Aug. 5, 2023

Language: Английский

---

Draft genome sequence of a halotolerant plant growth-promoting bacterium Pseudarthrobacter oxydans NCCP-2145 isolated from rhizospheric soil of mangrove plant Avicennia marina

Electronic Journal of Biotechnology, Journal Year: 2023, Volume and Issue: 66, P. 52 - 59

Published: Sept. 14, 2023

Limited knowledge exists regarding the diversity of mangrove plant-associated Pseudarthrobacter species. This study presents first draft genome and phylogenetic analysis a oxydans NCCP-2145, isolated from rhizospheric soil Aviciana marina, native plant found in Miani Hor, Lasbela-Baluchistan, Pakistan. The P. NCCP-2145 comprises 4,495,869 base pairs, with G+C content 65.9% 4,207 coding sequences. Genome annotation revealed presence multiple biosynthesis pathways. also identified genes responsible for growth-promoting traits, such as synthesis indole acetic acid, nitrogen fixation, phosphorus solubilization. Experimental evaluations confirmed strain positive reactions solubilization, indole-3-acetic acid production, ammonia production. Furthermore, exhibited tolerance to heavy metals (nickel, copper, cadmium) salinity levels up 10% NaCl. Antibiotic susceptibility testing indicated resistance only ceftazidime combination amoxicillin/clavulanic acid. For phylogenomic analysis, was analyzed compared closely related validly published type species DSM 20119T, revealing similarity score 98.64% based on 16S rRNA gene sequences 89.1% DNA-DNA hybridization, confirming its classification member species, oxydans. significantly contributes our understanding genomic characteristics, functional capabilities, potential attributes NCCP-2145. Future research should focus unraveling precise mechanisms underlying abilities exploring practical applications sustainable agriculture environmental restoration.

Language: Английский

---

The soil-microbe-plant resistome: A focus on the source-pathway-receptor continuum

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(9), P. 12666 - 12682

Published: Jan. 23, 2024

Language: Английский

---

Characterization of salt-tolerant diazotrophs with plant growth-promoting potential isolated from soda industry-affected technosoils

Plant and Soil, Journal Year: 2024, Volume and Issue: 503(1-2), P. 645 - 658

Published: March 8, 2024

Language: Английский

---