Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 212, P. 112914 - 112914
Published: March 30, 2024
Language: Английский
Saudi Pharmaceutical Journal, Journal Year: 2023, Volume and Issue: 32(1), P. 101918 - 101918
Published: Dec. 13, 2023
Chitosan is known to exert antimicrobial activity without the need for any chemical modification; however, new derivatives of chitosan can be created target multi-drug resistant bacteria. In this study, (CS) was cross-linked with sodium tripolyphosphate form nanoparticles, which were then coated polyacrylic acid (PAA). The SEM images revealed that CS-PAA nanoparticles had spherical shapes smooth surfaces and size dried approximately 222 nm. Biofilm formation significantly inhibited by 0.5 mg/mL CS-PAA. In-situ optical microscopy showed bacterial biofilm in Campylobacter jejuni, Pseudomonas aeruginosa, Escherichia coli after a single treatment 40 μg. Additionally, 20 µg demonstrated antibacterial against growth C. P. E. notable inhibitory zones 9, 12, 13 mm, respectively (P<0.01). development novel ecofriendly method preparation through an interaction PAA shows promise tool combat infections validates effective antibiofilm properties antibiotic pathogens.
Language: Английский
Citations
18
Chemistry & Biodiversity, Journal Year: 2024, Volume and Issue: 21(3)
Published: Feb. 9, 2024
Abstract Fungi are microorganisms of significant biotechnological importance due to their ability provide food and produce several value‐added secondary metabolites enzymes. Its products move billions dollars in the pharmaceutical, cosmetics, additives sectors. These also play a notable role bionanotechnology, leading production hybrid biological‐inorganic materials (such as cyborg cells) use enzyme complex biosynthesis nanoparticles. In this sense, optimizing fungal growth process is necessary, with selecting cultivation medium one essential factors for microorganism reach its maximum metabolic expression. The culture medium‘s composition can impact nanomaterial's stability prevent incorporation nanoparticles into cells. Therefore, our main objectives following: (1) compile discuss most commonly employed media formation cells, accompanied by preparation methods; (2) six‐step guide investigating metabolomic profile (3) procedures microbial
Language: Английский
Citations
6
Water Research, Journal Year: 2024, Volume and Issue: 256, P. 121646 - 121646
Published: April 19, 2024
Language: Английский
Citations
6
Biomass and Bioenergy, Journal Year: 2023, Volume and Issue: 180, P. 106996 - 106996
Published: Nov. 28, 2023
Language: Английский
Citations
13
ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(9), P. 2684 - 2692
Published: Aug. 20, 2024
Advances in genome engineering and associated technologies have reinvigorated natural products research. Here we highlight the latest developments field across discover–design–build–test–learn cycle of bioengineering, from recent progress computational tools for AI-supported mining, enzyme pathway engineering, compound identification to novel host systems new techniques improving production levels, place these trends context responsible research innovation, emphasizing importance anticipatory analysis at early stages process development.
Language: Английский
Citations
4
Applied Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 109(1)
Published: Jan. 20, 2025
Language: Английский
Citations
0
Fitoterapia, Journal Year: 2025, Volume and Issue: unknown, P. 106416 - 106416
Published: Feb. 1, 2025
Language: Английский
Citations
0
Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108528 - 108528
Published: Feb. 1, 2025
Cellular heterogeneity plays a crucial role in biotechnological processes, significantly influencing metabolic activity, product yield, and process consistency. This review explores the different dimensions of cellular heterogeneity, focusing on its manifestation at both single-cell population levels. The study examines how factors such as asymmetric cell division, age, environmental conditions contribute to functional diversity within populations, with an emphasis microorganisms like yeast. Age-related particular, is highlighted for impact pathways, mitochondrial function, secondary metabolite production, which directly affect bioprocess outcomes. Furthermore, discusses advanced techniques detecting managing including surface marker-based approaches, utilize proteins, polysaccharides, lipids, label-free methods that leverage volume physical properties separation. Understanding controlling essential optimizing industrial bioprocesses, improving ensuring quality. also underscores potential emerging tools, real-time analysis microfluidic devices, enhancing separation better efficiency robustness.
Language: Английский
Citations
0
Marine Drugs, Journal Year: 2025, Volume and Issue: 23(2), P. 69 - 69
Published: Feb. 7, 2025
Marine organisms represent a source of unique chemical entities with valuable biomedical potentialities, broad diversity, and complexity. It is essential to ensure reliable sustainable supply marine natural products (MNPs) for their translation into commercial drugs other products. From structural point view few exceptions, MNPs pharmaceutical importance derive from the so-called secondary metabolism organisms. When production strategies rely on macroorganisms, harvesting or culturing coupled extraction procedures frequently remain only alternative producing these compounds an industrial scale. Their can often be implemented laboratory scale cultures bacterial, fungal, microalgal sources. However, diverse approach, combining traditional methods modern synthetic biology biosynthesis strategies, must considered invertebrate MNPs, as they are usually naturally accumulated in very small quantities. This review offers comprehensive examination various addressing challenges related supply, synthesis, scalability. also underscores recent biotechnological advancements that likely transform current industrial-scale manufacturing pharmaceuticals derived
Language: Английский
Citations
0
Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15
Published: Feb. 25, 2025
Co-cultivation of microorganisms has emerged as a promising methodology for deciphering the intricate molecular interactions between species. This approach facilitates replication natural niches ecological or clinical relevance where microbes consistently interact. In this context, increasing attention been addressed toward elucidating crosstalk within fungal co-cultures. However, major challenge in area research is determining origin metabolites induced co-cultivation systems. Molecules elicited co-cultures may not be detectable individual cultures, making it challenging to establish which microorganism responsible their induction. For agar-diffused metabolites, imaging mass spectrometry can help overcome obstacle by localizing molecules during confrontations. volatile however, remains an open problem. To address issue, study, three-head-to-head co-culture strategy was developed, specifically focusing on exploration fungi via headspace solid-phase microextraction combined with gas chromatography spectrometry. applied study three species: Fusarium culmorum, Aspergillus amstelodami, and Cladosporium cladosporioides. The adopted revealed Fusarium-specific induction molecules: γ-terpinene two unidentified sesquiterpene compounds. Interestingly, showed antifungal activity bioassay against other amstelodami proposed could investigate highlight metabolite specific particular fungus involved vitro relevant better understanding complex biosynthetic responses consortia identifying activity.
Language: Английский
Citations
0