Growth balance analysis models of cyanobacteria for understanding resource allocation strategies DOI Creative Commons
Sajjad Ghaffarinasab, Martin J. Lercher, Hugo Dourado

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 18, 2023

Abstract Cyanobacteria have emerged as attractive microbial cell factories, they can convert atmospheric CO 2 and sunlight into valuable chemicals. To increase their growth productivity, one should aim to optimize the allocation of limited cellular resources across different metabolic processes. Here, we developed two balance analysis (GBA) models for cyanobacterium Synechocystis sp. PCC 6803. In its biological assumptions, closely related an existing coarse-grained model, while mathematical formulation is heavily streamlined. We show that GBA provide virtually identical predictions about resource among photosynthesis, carbon metabolism, protein translation machinery under environmental conditions previous, mathematically more involved model. Our model also captures effects photodamage on proteome resulting rates. further how be easily extended include reactions, leading a second capable new allocation. Balanced type presented here expanded details, providing useful toolbox understanding physiological capabilities cyanobacteria, resources, potential bioengineering optimized biomass production.

Language: Английский

Population-level heterogeneity complicates utilization of DOI
Lisa Yun, Jonathan K. Sakkos, Daniel C. Ducat

et al.

PubMed, Journal Year: 2024, Volume and Issue: 2024

Published: Jan. 1, 2024

Surface display technologies have been primarily developed for heterotrophic microbes, leaving photosynthetic counterparts like cyanobacteria with limited molecular tools. Here, we expanded upon surface systems in

Language: Английский

Citations

0
Advancing plastics bio-upcycling with photosynthetic microorganisms using bioengineering and bioconversion strategies DOI

Hazlam Shamin Ahmad Shaberi,

Hamidun Bunawan,

Sean Craig

et al.

Algal Research, Journal Year: 2024, Volume and Issue: unknown, P. 103755 - 103755

Published: Oct. 1, 2024

Language: Английский

Citations

0
Growth balance analysis models of cyanobacteria for understanding resource allocation strategies DOI Creative Commons
Sajjad Ghaffarinasab, Martin J. Lercher, Hugo Dourado

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 18, 2023

Abstract Cyanobacteria have emerged as attractive microbial cell factories, they can convert atmospheric CO 2 and sunlight into valuable chemicals. To increase their growth productivity, one should aim to optimize the allocation of limited cellular resources across different metabolic processes. Here, we developed two balance analysis (GBA) models for cyanobacterium Synechocystis sp. PCC 6803. In its biological assumptions, closely related an existing coarse-grained model, while mathematical formulation is heavily streamlined. We show that GBA provide virtually identical predictions about resource among photosynthesis, carbon metabolism, protein translation machinery under environmental conditions previous, mathematically more involved model. Our model also captures effects photodamage on proteome resulting rates. further how be easily extended include reactions, leading a second capable new allocation. Balanced type presented here expanded details, providing useful toolbox understanding physiological capabilities cyanobacteria, resources, potential bioengineering optimized biomass production.

Language: Английский

Citations

0