Published: Jan. 1, 2023
Language: Английский
Journal of Radioanalytical and Nuclear Chemistry, Journal Year: 2024, Volume and Issue: 333(4), P. 1779 - 1791
Published: March 11, 2024
Language: Английский
Citations
3
The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 912, P. 169242 - 169242
Published: Dec. 9, 2023
Research on eco-friendly bioremediation strategies for mitigating the environmental impact of toxic metals has gained attention in last years. Among all promising solutions, bentonite clays, to be used as artificial barriers isolate radioactive wastes within deep geological repository (DGR) concept, have emerged effective reservoir microorganisms with remarkable potential. The present study aims investigate fungi speciation and mobility selenium (Se) tellurium (Te), natural analogues 79Se 132Te waste, screen those strains potential context DGR. For this purpose, a multidisciplinary approach combining microbiology, biochemistry, microscopy was performed. Notably, Aspergillus sp. 3A demonstrated high tolerance Te(IV) Se(IV), evidenced by minimal inhibitory concentrations >16 >32 mM, respectively, along indexes. metalloid is mediated its capability reduce these mobile elements their elemental less soluble forms [Te(0) Se(0)], forming nanostructures various morphologies. Advanced electron techniques revealed intracellular Te(0) manifesting amorphous needle-like nanoparticles extracellular substantial microspheres irregular accumulations, characterized trigonal crystalline phase. Similarly, Se(0) exhibited diverse array morphologies, including hexagonal, irregular, needle-shaped structures, accompanied monoclinic formation through novel environmentally friendly processes suggests it would an excellent candidate contaminated environments, such vicinity repositories. It moreover holds immense recovery synthesis Te Se use numerous biotechnological biomedical applications.
Language: Английский
Citations
8
Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 15, 2024
Language: Английский
Citations
3
Journal of Hazardous Toxic and Radioactive Waste, Journal Year: 2024, Volume and Issue: 28(2)
Published: Jan. 24, 2024
In the long-term operation of any deep geological repository (DGR), microbial species can grow within and biological activity may take place. The presence microbes result in production small organic acids siderophores that change properties buffer material corrode metal canisters. current study, bacterial population Bikaner Barmer bentonites was investigated through plate culture technique. For detailed analysis, 16S ribosomal RNA (rRNA) gene sequencing technique carried out. results showed Bacillus tequilensis KTCT 13622(T) Lysinibacillus fusiformis NBRC 15717(T) were present bentonite, whereas Burkholderia contaminans LMG 23361(T) Staphylococcus pasteuri ATCC 51129(T) bentonite. are rod-shaped, gram-positive, nonmoveable bacteria phylum Firmicutes. is a gram-negative, moveable bacterium Proteobacteria. genus gram-positive Bacteria belonging to Firmicutes restrict mobility toxic radionuclides by process biosorption; Actinobacteria have ability their shape extending out branches, or filaments, destabilizing compactness backfill material. identified would be helpful for designing DGRs, considering safety aspects.
Language: Английский
Citations
2
Journal of Solid State Chemistry, Journal Year: 2024, Volume and Issue: 335, P. 124708 - 124708
Published: April 8, 2024
Language: Английский
Citations
2
Microorganisms, Journal Year: 2024, Volume and Issue: 12(5), P. 1025 - 1025
Published: May 19, 2024
Currently, the production of radioactive waste from nuclear industries is increasing, leading to development reliable containment strategies. The deep geological repository (DGR) concept has emerged as a suitable storage solution, involving underground emplacement within stable formations. Bentonite clay, known for its exceptional properties, serves critical artificial barrier in DGR system. Recent studies have suggested stability bentonite relevant conditions, indicating potential enhance long-term safety performance repository. On other hand, due high resistance corrosion, copper one most studied reference materials canisters. This review provides comprehensive perspective on influence conditions characteristics and properties engineered barriers. paper outlines how evolving physico-chemical parameters (e.g., temperature, radiation) may impact these barriers over lifespan emphasizes significance understanding microbial processes, especially event radionuclide leakage U, Se) or canister corrosion. Therefore, this aims address future DGRs, which given complexity such systems.
Language: Английский
Citations
2
Environmental Pollution, Journal Year: 2024, Volume and Issue: 358, P. 124491 - 124491
Published: July 2, 2024
The deep geological repository (DGR) concept consists of storing radioactive waste in metal canisters, surrounded by compacted bentonite, and placed deeply into a formation. Here, bentonite slurry microcosms with copper inoculated bacterial consortium amended acetate, lactate sulfate were set up to investigate their geochemical evolution over year under anoxic conditions. impact microbial communities on the corrosion canisters an early-stage (45 days) was also assessed. electron donors/acceptor accelerated activity, while heat-shocked process had retarding effect. partially oxidize which is subsequently consumed when depleted. Early-stage showed that reduced diversity Pseudomonas Stenotrophomonas dominating community. However, sulfate-reducing bacteria such as Desulfocurvibacter, Anaerosolibacter, Desulfosporosinus enriched coupling oxidation lactate/acetate reduction sulfates. generated biogenic sulfides, could mediate conversion oxides (possibly formed trapped oxygen molecules or driven H2O) sulfide (Cu2S), identified X-ray photoelectron spectroscopy (XPS). Overall, these findings shed light ideal conditions would affect stability DGR barriers, emphasizing SRB gas generation, interaction components bentonite.
Language: Английский
Citations
2
The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 908, P. 168284 - 168284
Published: Nov. 2, 2023
Uranium (U) represents the preeminent hazardous radionuclide within context of nuclear waste repositories. Indigenous microorganisms in bentonite can influence speciation and migration Deep Geological Repositories (DGRs) for storage. While bacterial communities samples have been extensively studied, impact fungi has somewhat overlooked. Here, we investigate geomicrobiological processes microcosms amended with uranyl nitrate glycerol-2-phosphate (G2P) six-month incubation. ITS sequencing revealed that fungal community was mainly composed Ascomycota (96.6 %). The presence U enriched specific taxa, such as Penicillium Fusarium, potentially associated uranium immobilization mechanisms. Conversely, amendment into G2P-suplemented exhibited minimal impact, resulting a akin to control group. Several strains were isolated from explore their potential biomineralization, including Fusarium oxysporum, Aspergillus sp., spp., among others. High Annular Angle Dark-Field Scanning Transmission Electron Microscopy (HAADF) analyses showed capacity F. oxysporum B1 form U-phosphate mineral phases, likely mediated by phosphatase activity. Therefore, our study emphasizes need take account indigenous overall assessment microbial DGRs environments.
Language: Английский
Citations
6
Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 476, P. 135044 - 135044
Published: June 26, 2024
Deep geological repositories (DGRs) stand out as one of the optimal options for managing high-level radioactive waste (HLW) such uranium (U) in near future. Here, we provide novel insights into microbial behavior DGR bentonite barrier, addressing potential worst-case scenarios leakage (e.g., U) and groundwater infiltration electron rich donors bentonite. After a three-year anaerobic incubation, Illumina sequencing results revealed bacterial diversity dominated by spore-forming microorganisms mainly from phylum Firmicutes. Highly U tolerant viable isolates genera Peribacillus, Bacillus, some SRB Desulfovibrio Desulfosporosinus, were enriched U-amended The obtained XPS XRD showed that was present U(VI) U(IV) species. Regarding U(VI), have identified biogenic phosphates, U(UO2)·(PO4)2, located inner part cell membranes addition to U(VI)-adsorbed clays montmorillonite. Biogenic species uraninite may be produced result enzymatic reduction. These findings suggest under donor-rich water-saturation conditions, community can control speciation, immobilizing it, thus enhancing future safety if container rupture occurs.
Language: Английский
Citations
1
Geosystem Engineering, Journal Year: 2024, Volume and Issue: 27(5), P. 235 - 251
Published: July 10, 2024
Language: Английский
Citations
1