Aquaculture Reports,
Journal Year:
2024,
Volume and Issue:
35, P. 102004 - 102004
Published: Feb. 28, 2024
Nanoplastics
(NPs,
<
1
μm)
have
attracted
widespread
attention
due
to
their
potential
threats
organisms,
ecosystem,
and
public
health.
However,
there
is
limited
research
on
the
effects
of
NPs
cephalopods.
Sepia
esculenta
(Mollusks,
Cephalopoda,
Sepioidea),
an
economically
major
species
Sepiidea
family
in
world,
well
received
by
consumers
for
its
delicious
quality
rich
nutrition.
In
present
study,
S.
larvae
were
treated
with
polystyrene
nanoplastics
(PS-NPs)
at
50
μg/mL
4
h,
then
physiological
biomarkers
(SOD,
GSTs
MDA)
RNA-Seq
whole
body
performed.
Research
results
suggested
that
PS-NPs
exposure
had
induced
oxidative
stress
larvae.
addition,
several
key
genes
signaling
pathways
related
obtained
based
DEGs,
GO
KEGG
enrichment
analyses,
as
PPI
construction
analysis
transcriptome
data.
This
study
enriches
different
organisms
provides
insights
references
revealing
mechanisms
caused
molecular
level.
Ecotoxicology and Environmental Safety,
Journal Year:
2024,
Volume and Issue:
272, P. 116028 - 116028
Published: Feb. 3, 2024
Extensive
application
of
lead
(Pb)
brought
about
environmental
pollution
and
toxic
reactions
organisms.
Selenium
(Se)
has
the
effect
antagonizing
Pb
poisoning
in
humans
animals.
However,
it
is
still
unclear
how
causes
brainstem
toxicity.
In
present
study,
we
wanted
to
investigate
whether
Se
can
alleviate
toxicity
chicken
brainstems
by
reducing
apoptosis.
One
hundred
eighty
chickens
were
randomly
divided
into
four
groups,
namely
control
group,
Se/Pb
group.
Morphological
examination,
ultrastructural
observation,
relative
mRNA
expressions
genes
on
heat
shock
proteins
(HSPs);
selenoproteins;
inflammatory
cytokines;
apoptosis-related
factors
investigated.
The
results
showed
that
exposure
led
tissue
damage
apoptosis
brainstems.
Furthermore,
an
atypical
expression
HSPs
(HSP27,
HSP40,
HSP60,
HSP70,
HSP90);
selenoprotein
family
glutathione
peroxidase
(GPx)
1,
GPx2,
GPx3,
GPx4),
thioredoxin
reductases
(Txnrd)
(Txnrd1,
Txnrd2,
Txnrd3),
dio
famliy
(diodothyronine
deiodinases
(Dio)1,
Dio2,
Dio3),
as
well
other
selenoproteins
(selenoprotein
(Sel)T,
SelK,
SelS,
SelH,
SelM,
SelU,
SelI,
SelO,
Selpb,
n1
(Sepn1),
Sepp1,
Sepx1,
Sepw1,
15-kDa
(Sep15),
selenophosphate
synthetases
2
(SPS2));
cytokines
(Interleukin
(IL-2),
IL-4,
IL-6,
IL-12β,
IL-17,
Interferon-γ
(IFN-γ));
(B-cell
lymphoma-2
(Bcl-2),
tumor
protein
53
(p53),
Bcl-2
Associated
X
(Bax),
Cytochrome
c
(Cyt
c),
Caspase-3)
identified.
An
reaction
induced
after
Pb.
alleviated
abnormal
HSPs,
selenoproteins,
cytokines,
tissues
treated
with
indicated
inflammatory,
involved
Se-resisted
poisoning.
Overall,
had
resistance
against
poisoning,
be
act
antidote
for
Poultry Science,
Journal Year:
2024,
Volume and Issue:
103(4), P. 103483 - 103483
Published: Jan. 21, 2024
Salmonella
infection
is
a
major
concern
in
poultry
production
which
poses
potential
risks
to
food
safety.
Our
previous
study
confirmed
that
Lactiplantibacillus
plantarum
(LP)
postbiotic
exhibited
strong
antibacterial
capacity
on
vitro.
This
aimed
investigate
the
beneficial
effects
and
underlying
mechanism
of
LP
Salmonella-challenged
broilers.
A
total
240
one-day-old
male
yellow-feathered
broilers
were
pretreated
with
0.8%
MRS
medium
or
(LP
cell-free
culture
supernatant,
LPC)
drinking
water
for
28
days,
then
challenged
109
CFU
enterica
serotype
Enteritidis
(SE).
Birds
sacrificed
three
days
post
infection.
Results
showed
LPC
maintained
growth
performance
by
increasing
BW,
ADG
ADFI
under
SE
challenge.
significantly
attenuated
SE-induced
intestinal
mucosal
damages.
Specifically,
it
decreased
injury
score,
increased
villus
length
villus/crypt,
regulated
expression
injury-related
genes
(Villin,
MMP3,
I-FABP),
enhanced
tight
junctions
(ZO-1
Claudin-1).
caused
dramatic
inflammatory
response,
as
indicated
up-regulated
concentrations
IL-1β,
IL-6,
TNF-α,
downregulation
IL-10,
while
pretreatment
markedly
reversed
this
trend.
We
found
inhibited
activation
NLRP3
inflammasome
decreasing
gene
NLRP3,
Caspase‐1,
IL-lβ
IL-18.
Furthermore,
suppressed
inhibiting
NF-κB
signaling
pathway
(the
reduced
levels
TLR4,
MyD88,
IRAK4
NF-κB).
Finally,
our
results
gut
microbiota
enhancing
percentage
Ligilactobacillus
Alistipes
Barnesiella.
In
summary,
we
was
effective
protect
against
infection,
possibly
through
suppressing
optimizing
microbiota.
provides
postbiotics
prevention
poultry.
Toxics,
Journal Year:
2023,
Volume and Issue:
11(9), P. 734 - 734
Published: Aug. 25, 2023
Lead
(Pb),
a
hazardous
heavy
metal,
can
damage
the
health
of
organisms.
However,
it
is
not
clear
whether
Pb
chicken
cerebellums
and
thalami.
Selenium
(Se),
an
essential
nutrient
for
organisms,
has
palliative
effect
on
poisoning
in
chickens.
In
our
experiment,
model
chickens
treated
with
Se
alone
combination
was
established
to
investigate
molecular
mechanism
alleviating
Pb-caused
both
Our
morphological
results
indicated
that
caused
apoptotic
lesions,
such
as
mitochondrial
nuclear
damage.
Further,
anti-apoptotic
gene
Bcl-2
decreased;
contrary,
four
pro-apoptotic
genes
(p53,
Bax,
Cyt
c,
Caspase-3)
increased
under
treatment,
meaning
apoptosis
via
p53-Cyt
c-Caspase-3
pathway.
Furthermore,
we
further
demonstrated
elevated
HSPs
(HSP27,
HSP40,
HSP70,
HSP90),
well
HSP70
took
part
apoptosis.
addition,
found
exposure
led
oxidative
stress
up-regulating
oxidant
H2O2
down-regulating
antioxidants
(CAT,
SOD,
GST,
GPx).
Moreover,
decreased
three
Se-containing
factors
(Txnrd1,
Txnrd2,
Txnrd3),
confirming
stress.
Interestingly,
supplementation
reversed
above
changes
by
alleviated
Pb-induced
A
time
dependency
Bcl-2,
c
cerebellums,
CAT,
GPx,
p53
thalami
Pb-exposed
HSP27
were
more
sensitive
than
those
respectively,
exposure.
severe
cerebellums.
conclusion,
after
Txnrds
mediated
stress,
up-regulated
HSPs,
finally,
triggered
antagonized
pathway
selenoproteins
This
study
provides
new
information
environmental
pollutant
detoxification
abiotic
