Postbiotic mediators derived from Lactobacillus species enhance riboflavin-mediated antimicrobial photodynamic therapy for eradication of Streptococcus mutans planktonic and biofilm growth

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 24, 2024

Streptococcus mutans has been implicated as a primary causative agent of dental caries and one its important virulence properties is an ability to form biofilm on tooth surfaces. Thus, strategies prevent control S. biofilms are requested. The present study aimed examine the eradication planktonic cells using riboflavin (Rib)-mediated antimicrobial photodynamic therapy (aPDT) enhanced by postbiotic mediators derived from Lactobacillus species.

Language: Английский

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In vitro antibacterial activity of photoactivated flavonoid glycosides against Acinetobacter baumannii

AMB Express, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 4, 2024

Acinetobacter baumannii's extensive antibiotic resistance makes its infections difficult to treat, so effective strategies fight this bacterium are urgently needed. This study aims evaluate the effectiveness of antimicrobial photodynamic therapy (aPDT) mediated by Rutin-Gal(III) complex and Quercetin against A. baumannii. Absorbance spectra, fluorescence minimum inhibitory concentration (MIC) were determined. The intracellular reactive oxygen species (ROS), extracellular polymeric substances (EPS), cell membrane permeability, expression ompA blaOXA−23, anti-biofilm activity, anti-metabolic activity complex- Quercetin-mediated aPDT measured. revealed absorption peaks in visible spectra. displayed at 524 nm 540 nm, respectively. MIC values for 64 µg/mL 256 µg/mL, Quercetin- complex-mediated significantly reduced colony forming units/mL (58.4% 67.5%), EPS synthesis (47.4% 56.5%), metabolic (30.5% 36.3%), (5.5- 10.5-fold), blaOXA−23 (4.1-fold 7.8-fold) genes (respectively; all P < 0.05). enhanced notable biofilm degradation (36.2% 40.6%), ROS production (2.55- 2.90-folds), permeability (10.8– 9.6-folds) findings indicate that exhibits antibacterial properties could serve as a valuable adjunctive strategy conventional treatments baumannii infections. One limitation is it was conducted solely on standard strain; testing clinical isolates would allow more reliable interpretation results.

Language: Английский

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Antibacterial efficacy of Doxycycline as a Photosensitizer in Photodynamic Therapy against Subgingival Plaque Bacteria

Research Journal of Pharmacy and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 4929 - 4933

Published: Oct. 22, 2024

Background: Periodontitis is an inflammation that occurs in the periodontium and caused by host immune responses subgingival plaque bacteria. Subgingival bacteria can initiate periodontal disease because they have potential to induce release of proinflammatory cytokines. Mechanical debridement alone not always possible completely remove pathogenic cause infections due accessibility location. New treatments, such as photodynamic therapy, which uses lasers, offer alternative option. Photodynamic therapy a non-invasive photons light energy for medical purposes. The mechanism based on triad consisting photosensitizer, source, molecular oxygen. Microorganisms first absorb then are activated specific wavelength. Photosensitizer transfer received oxygen convert into ROS, death microorganisms affecting their membranes, proteins, nucleic acids. Doxycycline widely used known act exogenous photosensitizer. Objective: aim research was evaluate doxycycline photosensitizer against Method: diffusion method test bacterial inhibition with 4 replications. This divided 6 groups: (1) control, (2) (3) 0,0125% (4) 0,025% (5) 0,05% (6) 0,1% therapy. were collected from stock put reaction tube contained liquid BHIB. After that, culture incubated 48hours at 37ºC, planted Mueller Hinton agar spreading technique. Filter paper inserted each concentrations placed petridish has been wavelength 405nm 30 seconds exposed paper. Then, plates 48 hours 37ºC. Result: avarage zone obtained following 0mm, 13.375mm, 14.6125mm, 15.450mm, 17.325mm, 19.2875mm. Conclusion: 0.1% concentration group combined biggest

Language: Английский

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Anti-biofilm effect of clove oil against Candida albicans: A systematic review

Journal of Oral and Maxillofacial Pathology, Journal Year: 2024, Volume and Issue: 28(4), P. 665 - 671

Published: Oct. 1, 2024

In recent years, Candida albicans has emerged as a significant opportunistic pathogen, causing various infections, particularly in immunocompromised geriatric individuals. One of the major hindrances managing infections lies its potential to develop and survive biofilms, which demonstrate resistance conventional antifungal therapies, thereby complicating treatment strategies. While clove oil shown promising results agent, specific anti-biofilm effects against biofilms remain relatively underexplored. Therefore, there is need for comprehensive review evaluate existing literature on properties mechanisms action biofilms. Such will provide valuable insights into therapeutic applications combating biofilm-associated infections. This enable us infer understand albicans. systematic aims determine efficacy was registered 'PROSPERO' (CRD42024524134) followed Preferred Reporting Items Systematic Reviews Meta-Analyses guidelines. The searches used Google Scholar, PubMed, Science Direct databases. All original studies published English from 2004 2023 were included. From 37 found online, only four reviewed. Results showed that had activity when assessed Our provides oil's agent Clove also represents avenue future research development

Language: Английский

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