The causal relationship between gut microbiota and biliary tract cancer: comprehensive bidirectional Mendelian randomization analysis

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: March 15, 2024

Background Growing evidence has shown that gut microbiome composition is associated with Biliary tract cancer (BTC), but the causality remains unknown. This study aimed to explore causal relationship between microbiota and BTC, conduct an appraisal of microbiome’s utility in facilitating early diagnosis BTC. Methods We acquired summary data for Genome-wide Association Studies (GWAS) pertaining BTC (418 cases 159,201 controls) from Biobank Japan (BBJ) database. Additionally, GWAS relevant (N = 18,340) were sourced MiBioGen consortium. The primary methodology employed analysis consisted Inverse Variance Weighting (IVW). Evaluations sensitivity carried out through utilization multiple statistical techniques, encompassing Cochrane’s Q test, MR-Egger intercept evaluation, global test MR-PRESSO, a leave-one-out methodological analysis. Ultimately, reverse Mendelian Randomization was conducted assess potential reciprocal causality. Results outcomes derived IVW substantiated presence Family Streptococcaceae (OR 0.44, P 0.034), Veillonellaceae 0.46, 0.018), Genus Dorea 0.29, 0.041) exerted protective influence against Conversely, Class Lentisphaeria 2.21, 0.017), Lachnospiraceae FCS020 Group 2.30, 0.013), Order Victivallales 0.017) adverse impact. To any effect, we used as exposure outcome, this revealed associations five different types microbiota. disclosed absence empirical indicators either heterogeneity or pleiotropy. Conclusion investigation represents inaugural identification indicative supporting beneficial detrimental relationships risk determined MR methodologies. These could hold significance formulation individualized therapeutic strategies at prevention survival enhancement.

Language: Английский

---

Reducing the Risk of Pre-Eclampsia in Women with Polycystic Ovary Syndrome Using a Combination of Pregnancy Screening, Lifestyle, and Medical Management Strategies

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(6), P. 1774 - 1774

Published: March 20, 2024

Polycystic ovary syndrome (PCOS) is a multisystem disorder that presents with variety of phenotypes involving metabolic, endocrine, reproductive, and psychological symptoms signs. Women PCOS are at increased risk pregnancy complications including implantation failure, miscarriage, gestational diabetes, fetal growth restriction, preterm labor, pre-eclampsia (PE). This may be attributed to the presence specific susceptibility features associated before during pregnancy, such as chronic systemic inflammation, insulin resistance (IR), hyperandrogenism, all which have been an complications. Many reversible following lifestyle interventions diet exercise, pregnant women healthy found lower complications, PE. narrative synthesis summarizes evidence investigating PE role nutritional factors in PCOS. The findings suggest beneficial aspects management PCOS, recommended evidence-based international guidelines, extend improved outcomes. Identifying high-risk will allow targeted interventions, early-pregnancy screening, surveillance for should included assessment algorithms

Language: Английский

---

Investigating casual association among gut microbiome and esophageal cancer: A Mendelian randomization study

Medicine, Journal Year: 2025, Volume and Issue: 104(8), P. e41563 - e41563

Published: Feb. 21, 2025

The gut microbiota has been strongly linked to gastrointestinal cancer, but the relationship between and esophageal cancer (EC) is still not fully understood. We conducted a 2-sample Mendelian randomization (MR) study unveil potential impact of intestinal microorganisms on EC in East Asian populations. In order delve deeper into causal EC, we MR analysis, utilizing 211 single nucleotide polymorphisms associated with microbiota, sourced from largest genome-wide association for our analysis. To estimate relationship, employed inverse variance weighting method. addition, assess influence pleiotropy, used MR-Egger regression Among 10 specific bacterial taxa identified using as being risk observed positive family Bacteroidaceae ( P = .04), genus Bacteroides Bilophila .02), Candidatus Soleaferrea .02) while Victivallaceae .03), Eubacterium coprostanoligenes .01), Catenibacterium Coprococcus2 unknowngenus.id.959 unknowngenus.id.1868 .01) may be reduced EC. Our analysis indicate probable development advancement These findings offer novel perspectives possible application targeted bacteria prevention management

Language: Английский

---

All Properties of Infertility Microbiome in a Review Article

Journal of Clinical Laboratory Analysis, Journal Year: 2025, Volume and Issue: unknown

Published: March 9, 2025

The microbiome is crucial for many physiological processes, including immunity, metabolism, and reproduction. This review aims to contribute a detailed understanding of the genital tract, which can lead better management dysbiosis reproductive disorders. Data from four international information databases Medline, Scopus, Embase, Google Scholar. search strategy was based on combination following terms: "microbiota," "microbiome," "microfilm," "microflora," "fertility," or "infertility." advent next-generation sequencing-based technologies during last decade has revealed presence microbial communities in nearly every part human body, system. Several studies have shown significant differences between microbiota vagina endometrium, as well other parts upper tract. plays critical role determining person's health state, tract may impact fertility potential before after assisted treatments (ARTs). To completely understand microbiome, future research should focus not only description but also interaction bacteria, production biofilms, microorganisms with cells.

Language: Английский

---

Telomere Length as Both Cause and Consequence in Type 1 Diabetes: Evidence from Bidirectional Mendelian Randomization

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 774 - 774

Published: March 22, 2025

Background/Objectives: Diabetes is the most prevalent metabolic disease globally, characterized by dysregulated glucose control and accompanied multiple refractory complications. As a critical marker of cellular homeostasis, telomere length (TL) may be associated with progression diabetes. However, causal relationship between diabetes TL remains unclear, particularly whether homeostasis imbalance acts as consequence diabetic complications or precipitating factor in development. Methods: We performed bidirectional Mendelian randomization (MR) analysis using genome-wide association study (GWAS) data. Following three core assumptions MR analysis, we conducted quality on all instrumental variables to ensure methodological rigor. The inverse variance weighted (IVW) method served primary analytical method, supplemented additional methods evaluate significance results. Furthermore, sensitivity analyses reliability robustness findings. Results: Forward revealed that shortened significantly increases risk broadly defined Type 1 (T1D) unspecified types (p < 0.05). Additionally, identified positive several diabetes-related complications, including co-morbidities, nephropathy, ketoacidosis Interestingly, reverse demonstrated effect T1D its 0.05); however, this disappeared after adjusting for insulin use > Conclusions: Bidirectional complex T1D, where telomeres increase while itself trigger compensatory mechanisms affecting maintenance, playing crucial regulatory role relationship. These findings suggest biology fundamentally involved pathogenesis could inform future therapeutic approaches.

Language: Английский

---

Analyzing the causal relationship between gut microbiotas, blood metabolites, and COVID-19 susceptibility: A Mendelian randomization study

Medicine, Journal Year: 2025, Volume and Issue: 104(14), P. e41445 - e41445

Published: April 4, 2025

Gut microbiota and blood metabolites play crucial roles in the progression outcomes of COVID-19, but causal relationships mechanisms remain unclear. Our aim is to use two-sample Mendelian randomization (MR) explore between gut microbiota, COVID-19 susceptibility, potential mediating metabolites. We utilized summary statistics from largest genome-wide association studies (GWAS) date on (n = 18,340), 115,078), susceptibility (cases n 60,176 controls 1310,725 Host Genetics Initiative meta-analysis). conducted bidirectional MR analyses performed two-step identify Five analytical methods were used assess relationships, with inverse variance weighted (IVW) being primary method. Sensitivity also ensure robustness main results. Using IVW method, we found 3 types 34 susceptibility. In MR, non-oxidative branch Pentose phosphate pathway was shown reduce Sebacate (C10-DC) levels, species Parabacteroides goldsteinii negatively correlated Acetoacetate levels. levels associated while positively Furthermore, these remained significant after correcting for false discovery rates (all q-values < 0.05). Heterogeneity pleiotropy tests showed no statistical significance ( P > .05). Mediation analysis indicated that abundance mediated by (mediation proportion 15.8%), 31.7%). The current study provides evidence supporting several specific microbiotas as well findings warrant further validation through larger epidemiological studies.

Language: Английский

---

Gut microbiota and autism spectrum disorders: a bidirectional Mendelian randomization study

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Dec. 14, 2023

Background In recent years, observational studies have provided evidence supporting a potential association between autism spectrum disorder (ASD) and gut microbiota. However, the causal effect of microbiota on ASD remains unknown. Methods We identified summary statistics 206 from MiBioGen study, data were obtained latest Psychiatric Genomics Consortium Genome-Wide Association Study (GWAS). then performed Mendelian randomization (MR) to determine relationship using inverse variance weighted (IVW) method, simple mode, MR-Egger, median, model. Furthermore, we used Cochran’s Q test, MR-Egger intercept Randomization Pleiotropy RESidual Sum Outlier (MR-PRESSO), leave-one-out analysis identify heterogeneity pleiotropy. Moreover, Benjamin-Hochberg approach (FDR) was employed assess strength connection exposure outcome. reverse MR that found be causally associated with in forward examine relationships. The enrichment analyses analyze biological function at last. Results Based results IVW results, genetically predicted family Prevotellaceae genus Turicibacter had possible positive (IVW OR=1.14, 95% CI: 1.00-1.29, P =3.7×10 −2 ), four protective ASD: Dorea (OR=0.81, 0.69-0.96, =1.4×10 Ruminiclostridium5 =1.5×10 Ruminococcus1 (OR=0.83, 0.70-0.98, =2.8×10 Sutterella (OR=0.82, 0.68-0.99, =3.6×10 ). After FDR multiple-testing correction further observed there two still significant OR=1.24; 1.09-1.40, =9.2×10 -4 ) strongly positively correlated RuminococcaceaeUCG005 OR=0.78, 0.67-0.89, =6.9×10 −4 negatively ASD. sensitivity excluded influence horizontal Conclusion Our findings reveal several microbiomes These deepen our comprehension role ASD’s pathology, providing foothold for novel ideas theoretical frameworks prevent treat this patient population future.

Language: Английский

---

Gut microbiota and polycystic ovary syndrome, focus on genetic associations: a bidirectional Mendelian randomization study

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 22, 2024

Background The contribution of gut microbiota to the pathogenesis polycystic ovary syndrome (PCOS) is controversial. causal relationship this question worth an in-depth comprehensive known single nucleotide polymorphisms associated with microbiota. Methods We conducted bidirectional Mendelian randomization (MR) utilizing instrumental variables (N = 18,340) from MiBioGen GWAS assess their impact on PCOS risk in FinnGen (27,943 cases and 162,936 controls). Two-sample MR using inverse variance weighting (IVW) was undertaken, followed by weighted median, mode, MR-Egger regression. In a subsample, we replicated our findings meta-analysis consortium (10,074 103,164 controls) European ancestry. Results IVWMR results suggested that six were causally features. After adjusting BMI, SHBG, fasting insulin, testosterone, alcohol intake frequency, effect sizes significantly reduced. Reverse analysis revealed effects features 13 no longer remained significant after sensitivity Bonferroni corrections. replication consistent suggest likely not independent cause PCOS. Conclusion Our did support relationships between at genetic level. More genome-wide association studies are warranted confirm relationship. Declaration This study contains 3533 words, 0 tables, figures text as well night supplementary files Supplementary material.

Language: Английский

---

The modern pathogenetic challenges of polycystic ovary syndrome

Reproductive health of woman, Journal Year: 2024, Volume and Issue: 2, P. 75 - 80

Published: March 9, 2024

Polycystic ovary syndrome (PCOS) is one of the neuroendocrine syndromes in women reproductive age. In pathogenesis its development a great importance paid to hormonal disbalance, metabolic changes, overweight and obesity, diabetes mellitus, insulin resistance, genetic factors, etc. Very often, PCOS accompanied by infertility, cardiovascular risks, mellitus.The article devoted review modern scientific literature about last researches mechanisms disorders which are connected with also, psychological disturbance stress. The describes specific conditions patients. On basis analyzed publications connection between disorders, overweight, obesity lipidogram parameters presented.Also, gut vaginal microbiota described this paper. studies demonstrate significant changes human microbiome course polycystic syndrome. presents concepts difference composition vagina syndrome.The pathogenetic role processes pathogenesis, namely, regulation immune response inflammation pathology, described. It has been shown that there differences microflora intestines, manifested decreased concentration Lactobacillus presence variety other microorganisms compared healthy women.Thus, main links closely each other. That why multimodal approaches for study management patients extremely important.

Language: Английский

---

Gut microbiota in women with polycystic ovary syndrome: an individual based analysis of publicly available data

EClinicalMedicine, Journal Year: 2024, Volume and Issue: 77, P. 102884 - 102884

Published: Oct. 18, 2024

Language: Английский

---