Association between dietary vitamin E intake and constipation: NHANES 2005–2010

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Aug. 20, 2024

This investigation aimed to analyze the association between dietary vitamin E intake and constipation prevalence among United States adults.

Language: Английский

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Gut microbiome and plasma metabolome alterations in myopic mice

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 21, 2023

Background Myopia is one of the most common eye diseases leading to blurred distance vision. Inflammatory could trigger or exacerbate myopic changes. Although gut microbiota bacteria are associated with various inflammatory diseases, little known about its role in myopia. Materials and methods The mice were randomly divided into control model groups, group being attached-30D lens onto eyes for 3 weeks. Then, mouse cecal contents plasma collected analyze their intestinal metabolome. Results We identified that microbial composition differed considerably between non-myopic mice, relative abundance Firmicutes phylum decreased obviously while Actinobacteria was increased Furthermore, Bifidobacterium positively correlated axial lengths (ALs) eyeballs negatively refractive diopters. Untargeted metabolomic analysis 141 differentially expressed metabolites, Kyoto Encyclopedia Genes Genomes pathway enrichment revealed considerable mainly amino acid metabolism pathways. Notably, pathways involved glutamate including “Glutamine D-glutamate metabolism” “Alanine, aspartate changed dramatically, which presented as concentrations L-Glutamate L-Glutamine Interestingly, microbiome dysbiosis metabolites alternations myopia have a disrupting barrier feature. further demonstrated function impaired manifesting expression Occludin, ZO-1 permeation FITC-dextran. Discussion Myopic had altered profiles compared mice. metabolomics shift an interrupting Our study provides new insights possible reinforces potential feasibility microbiome-based therapies

Language: Английский

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Associations between the oxidative balance score and constipation: a cross-sectional study of the NHANES, 2005–2010

BMC Public Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 16, 2024

Abstracts Objective The oxidative balance score (OBS) reflects the overall burden of stress in an individual, with a higher OBS indicating greater antioxidant exposure. This study aimed to explore association between constipation and OBS. Methods Variables were extracted from participants who completed questionnaire as part National Health Nutrition Examination Survey (NHANES) 2005 2010. was developed based on dietary lifestyle factors, encompassing 16 nutrients 4 variables. Weighted logistic regression restricted cubic spline (RCS) analyses employed evaluate constipation. Results After adjusting for all covariates, weighted multivariate analysis revealed 4% reduction incidence each additional unit (OR: 0.96, 95% CI: 0.95–0.97, p < 0.001). In subgroup, risk significantly decreased compared that lowest quartile (Q2: 0.72, P = 0.024; Q3: 0.59, 0.001; Q4: 0.54, Conclusions present demonstrated significant (OBS), particularly OBS, increase may reduce developing constipation, which play important role. finding suggested modification could be approach preventing

Language: Английский

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Study on the effects of Massa Medicata Fermentata with different formulations on the intestinal microbiota and enzyme activities in mice with spleen deficiency constipation

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 7, 2025

This study aims to explore the therapeutic mechanism of Massa Medicata Fermentata (MMF) with different formulations on spleen deficiency constipation in mice by analyzing gastrointestinal hormones, D-xylose, intestinal microbiota, and enzyme activities. A model was established using an oral administration Sennae Folium decoction combined controlled diet water intake. After successful establishment, were treated MMF S1, S2, S3. Following intervention, serum samples from each group collected measure VIP, 5-HT, D-xylose. Additionally, small intestine contents analyzed for activity subjected 16S rRNA high-throughput sequencing. Mice showed significant decreases body weight fecal content. In contrast, CS2 CS3 groups returned normal levels, content also normal. The S2 S3 significantly increased protease sucrase enzymes levels compared CM group. Serum D-xylose reduced VIP but decreased groups. 5-HT CS1 significantly, returning showing increases. sequencing analysis revealed that all three effectively restored microbiota composition mice. LEfSe identified characteristic linked intervention upregulated chloroalkane chloroalkene degradation vibrio cholerae pathogenic cycle pathways Candidatus_Arthromitus Psychrobacter positive negative correlations respectively. alleviated symptoms modulating activities, restoring balance. as potential biomarkers treatment constipation. Different have mechanisms regulating through microbiota.

Language: Английский

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Gut-brain axis mediated by intestinal content microbiota was associated with Zhishi Daozhi decoction on constipation

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 3, 2025

Background Constipation is a common digestive system disorder, which closely related to the intestinal flora. Zhishi Daozhi decoction (ZDD) traditional Chinese medicine prescription used treat constipation caused by indigestion. This study evaluate efficacy of ZDD in treating and elucidate underlying mechanism. Methods In this study, Kunming mice were administered high-protein diet (HFHPD) loperamide hydrochloride injections induce constipation. The then received varying doses (2.4, 4.7, 9.4 mg/kg) for seven days. Following sampling process, we measured fecal microbial activity. levels 5-hydroxytryptamine (5-HT), vasoactive peptide (VIP), aquaporin-3 (AQP3) quantified using enzyme-linked immunosorbent assay. Changes gut microbiota evaluated through 16S rRNA gene sequencing. Additionally, investigated correlation between specific features 5-HT, VIP, AQP3. Results surface model group (CMM) was rough dry. stool low-dose (CLD), medium-dose (CMD), high-dose (CHD) exhibited smoother texture, resembling that normal (CNM). 5-HT CMM significantly lower than CNM, CLD, CHD. VIP CMD other four groups, AQP3 showed decreasing trend. activity higher groups. Diversity analysis indicated CHD treatments more effective restoring structure. Potential pathogenic bacteria, including Clostridium , Aerococcus Jeotgalicoccus Staphylococcus enriched CMM. contrast, beneficial bacteria such as Faecalibacterium Bacillaceae Bacillus prevalent CMD, Correlation revealed Streptococcus Enterococcus positively correlated with while Succinivibrio negative 5-HT. Conclusions induced HFHPD disrupts structure microbiota. appears alleviate constipation, potentially mechanisms linked brain-gut axis its interaction Among treatment medium dose demonstrated most results.

Language: Английский

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Causal Link between Gut Microbiota and Myopia—A Two-Sample Mendelian Randomization Analysis

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(03), P. 1063 - 1074

Published: Jan. 1, 2025

Language: Английский

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Lactitol Alleviates Loperamide-Induced Constipation in Sprague Dawley Rats by Regulating Serotonin, Short-Chain Fatty Acids, and Gut Microbiota

Foods, Journal Year: 2024, Volume and Issue: 13(13), P. 2128 - 2128

Published: July 3, 2024

The objective of this study was to examine the impact lactitol on constipation caused by loperamide in Sprague Dawley rats, with a particular emphasis its underlying mechanisms and potential health advantages. effectively improved fecal parameters, intestinal tissue structure, expression constipation-related gene proteins. Lactitol alleviated weight water content altered enhanced gastrointestinal transit. administration also restored mucosal muscular layer thickness. Mechanistically, upregulated mRNA and/or protein levels mucins (MUC2 MUC4), occludin, claudin-1, zonula occludens, indicating barrier function. positively regulated composition cecal microbiota, leading an increased relative abundance Bifidobacterium, Lactobacillus, Romboutsia. Conversely, decreased Prevotella, Aerococcus, Muribaculum, Blautia, Ruminococcus. This demonstrated relieve modulating gut microbiota. These findings suggest that is alternative traditional laxatives has as health-promoting food sweetener.

Language: Английский

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External damp environment aggravates diarrhea in spleen deficiency and dampness syndrome in mice: involvement of small intestinal contents microbiota, energy metabolism, gastrointestinal and fluid functions

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Oct. 31, 2024

Recent studies have increasingly demonstrated that a multiplatform water environment combined with lard gavage is an effective method for establishing mouse model of diarrhea. However, the interactions between intestinal microorganisms and diarrhea, as well relationships among energy metabolism, fluid balance, gastrointestinal function in this model, remain poorly understood.

Language: Английский

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