Metarhizium rileyi with broad‐spectrum insecticidal ability confers resistance against phytopathogens and insect pests as a phytoendophyte

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(7), P. 3246 - 3257

Published: Feb. 15, 2024

Abstract BACKGROUND Entomophagous fungi (EPF) not only directly kill insect pests, but also colonize plants and improve their resistance against pests. However, most previous research has focused on Beauveria bassiana Metarhizium anisopliae , there are few reports whether other EPF can enhance pests via endogenous colonization. Herein, an strain was isolated from diseased larvae of Spodoptera litura in a soybean field, subjected to genome‐wide sequencing at the chromosomal level. The pathogenicity isolate toward various pest insects evaluated, ability induce phytopathogens tested. RESULTS purified identified as M. rileyi designated MrS1Gz1–1. Biological assays revealed its strong five belonging Lepidoptera Hemiptera. Furthermore, inhibited growth soil‐borne plant disease caused by Sclerotinia sclerotiorum vitro . It colonized endophyte soil application, thereby inducing resistance‐related genes phytopathogen infection, it disrupted feeding selectivity S. larvae. CONCLUSION MrS1Gz1–1 potential broad‐spectrum microbial control agent that endotype. complete genome provides valuable resource for exploring host interactions. © 2024 Society Chemical Industry.

Language: Английский

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The Antagonistic Activity of Beneficial Fungi and Mechanisms Underlying Their Protective Effects on Plants Against Phytopathogens

Sustainability, Journal Year: 2025, Volume and Issue: 17(2), P. 450 - 450

Published: Jan. 9, 2025

Among the different strategies of plant protection from phytopathogens, use beneficial fungi has been described as a sustainable, eco-friendly approach. The aim present work was to evaluate antagonistic activity fungal strains in vitro and vivo. studied (Beauveria bassiana T7, Beauveria T15, Metarhizium robertsii An1, Talaromyces pinophilus T14) had pronounced against three phytopathogens (the growth inhibition 18.2–51%). In pot experiments, significantly reduced level stress barley plants caused by phytopathogenic load. effect consisted an increase morphometric parameters positive on photosynthetic pigments proline levels. 1-aminocyclopropane-1-carboxylate deaminase varied 0.95 2.73 µM α-KB mg protein−1 h−1. most significant mechanisms action M. An1 strain were following: production hydrolytic enzymes (chitinase glucanase amounted 0.23 U mL−1 3.42 mL−1, respectively) synthesis soluble volatile non-volatile compounds with antifungal properties, including destruxin E, A, hydroxyanthraquinones. results obtained revealed potential for their integration into sustainable agricultural system.

Language: Английский

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Bioinoculant-induced plant resistance is modulated by interactions with resident soil microbes

Environmental Microbiome, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 18, 2025

Entomopathogenic fungi are increasingly used as bio-inoculants to enhance crop growth and resistance. When applied rhizosphere soil, they interact with resident soil microbes, which can affect their ability colonize induce resistance in plants well modify the structure of microbiome, either directly through interactions or indirectly, mediated by plant. The extent such direct versus indirect between microbes impact microbe-induced crops remains unclear. This study uses a split-root system examine effects (plant-mediated) an entomopathogenic fungus, Metarhizium brunneum, on induced tomato against two-spotted spider mites. Additionally, explores how these influence composition diversity fungal bacterial communities. Resident reduced efficacy M. brunneum reduction occurred not only when interacted bio-inoculant but also were spatially separated within root system, indicating plant-mediated effects. inoculation did microbial led changes community composition, even communities contact inoculant. research highlights both bio-inoculant-induced pest underscores importance assessing potential adverse native

Language: Английский

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A strain of Talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plants

Biological Control, Journal Year: 2025, Volume and Issue: unknown, P. 105703 - 105703

Published: Jan. 1, 2025

Language: Английский

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Harnessing Fungal Bioagents Rich in Volatile Metabolites for Sustainable Crop Protection: A Critical Review

Journal of Basic Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

ABSTRACT Pests and diseases have a significant impact on crop health yields, posing serious threat to global agriculture. Effective management strategies, such as integrated pest (IPM), including rotation, use of synthetic pesticides, biological control, resistant/tolerant varieties, are essential mitigate these risks ensure sustainable agricultural practices. Fungal bioagents play an important role in managing phytopathogens insect pests by acting agents. They promote healthy plant growth enhancing the uptake nutrients combating systemic resistance plants. Furthermore, fungal environmentally friendly, reducing application fungicides insecticides minimizing their negative crops environment. Their IPM promotes agriculture ensures high‐quality while maintaining soil microbial biodiversity. These rich sources volatile organic compounds (VOCs), which communication during interaction with phytopathogens. In management, VOC production beneficial fungi is accountable for efficacy against pathogens. Thus, this review discusses producing VOCs, extraction methods VOC, VOC‐producing disease knowledge gaps, future research areas.

Language: Английский

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The PKS–NRPS Gene BBA_09856 Deletion Mutant of Beauveria bassiana Enhanced Its Virulence Against Ostrinia furnacalis Larvae and Strengthened the Host Plant’s Resistance to Botrytis cinerea as an Endotype

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(3), P. 197 - 197

Published: March 4, 2025

Nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) play crucial roles in the development pathogenicity of entomopathogenic fungus Beauveria bassiana. However, they are among few biosynthetic gene clusters with unknown functions B. To investigate role hybrid PKS–NRPS BBA_09856 bassiana, we constructed a mutant strain, ∆BBA09856-WT, by deleting through Agrobacterium-mediated transformation. We then analyzed biological characteristics strain virulence toward Ostrinia furnacalis larvae, as well its antagonistic effects against phytopathogen Botrytis cinerea. found that average growth rate three strains, was significantly higher compared to wild-type (WT) on 15th day culture potato dextrose agar (PDA) plates (7.01 cm vs. 6.30 cm, p < 0.01). Additionally, spore production(3.16 × 107/cm2 9.95 106/cm2, 0.001) germination (82.50% 54.72%, 12 h, were different between WT strain. The survival rates O. infected after 8 days 61.66%, 30.00%, respectively, indicating tested strains greater than results dual test indicated inhibitory ∆BBA09856-WT cinerea 40.25% 47.65%, respectively (p 0.001). Similarly, test, reduced 9.90%, while exhibited inhibition 28.29% 0.05). diameters disease spots, measured 6 d inoculation tomato treatment groups, revealed significant differences endophytic colonization WT+Bc ∆BBA09856-WT+Bc groups (15.26 mm 12.16 mm, Notably, enhanced larvae increased activity Our indicate may have negative correlation bassiana insect pest antagonism These findings suggest molecular techniques, such editing, could be employed develop superior for control plant diseases pests.

Language: Английский

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Evaluating Beauveria bassiana Strains for Insect Pest Control and Endophytic Colonization in Wheat

Insects, Journal Year: 2025, Volume and Issue: 16(3), P. 287 - 287

Published: March 10, 2025

Certain entomopathogenic fungi, such as Beauveria bassiana, are highly pathogenic to arthropod pests and able colonize plant tissues, thereby enhancing both growth disease resistance. This study assessed three B. bassiana strains (CBM1, CBM2, CBM3) for their pathogenicity toward insect larvae colonization potential in wheat. The insecticidal activity of the fungi against major lepidopteran Helicoverpa armigera, Spodoptera frugiperda, Mythimna separata, Plutella xylostella was determined. were then applied wheat plants using seed immersion soil drench methods; rates compared, impacts fungal on survival evaluated. results demonstrated that all effective reducing damage, with CBM1 exhibiting highest followed by CBM3 CBM2. particularly effective, a significantly higher rate achieved through drenching compared immersion. inoculation resulted increased height at 30 days after sowing (DAS) root length 15 DAS control group. CBM1-colonized mortality fall armyworm. research has enriched biological microbial resource pool highlights integrated pest management strategies.

Language: Английский

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Entomopathogenic Fungi Effectively Control Phorodon cannabis Aphid Population in Cannabis sativa Plants

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 931 - 931

Published: March 16, 2025

The rapid expansion of the cannabis industry in Canada post-legalization has heightened prevalence pests, particularly aphid Phorodon (P. cannabis), which poses significant threats to crop health. This study investigates immediate effects P. on Cannabis sativa (C. sativa) plants and explores biological control strategies utilizing entomopathogenic fungi. Fungal isolates Beauveria bassiana Metarhizium anisopliae were isolated from infected aphids, cultured, characterized. Infection tests aphids revealed that both fungi achieved 100% mortality at high conidial concentrations (1 × 107 conidia/mL) by 10th DAT, with demonstrating better efficacy. In greenhouse trials three varieties, B. effectively controlled populations, keeping levels low stable infested treated concentration 1 conidia mL−1 (I-B) insecticide (I-I). Both I-B I-I treatments maintained populations near zero for nine weeks. contrast, showed growth, Perseid variety being most susceptible, followed Congo Durban, while GCC exhibited lowest susceptibility. Cannabinoid terpene analyses treatment substantially decreased amount produced cannabinoids terpenes. bassiana-treated higher key metabolites, including delta-9-tetrahydrocannabinolic acid cannabidiolic acid, total terpenes, compared chemically plants, two out cultivars, these than control, untreated plants. findings highlight as an eco-friendly alternative pest management not only controls but also supports biochemical quality

Language: Английский

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Climate Change and Agroecosystem: How Entomopathogenic Fungi Influence Soil Microbial Diversity and Plant Growth?

Published: Jan. 1, 2025

Language: Английский

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Fusarium citri as an entomopathogenic fungus mediating plant resistance against insect pests and phytopathogens

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 15, 2025

Fusarium citri has been historically recognized as a phytopathogen but never an entomophagous fungus (EPF) with plant endogeneity. In the present study, F. strain, FcS1GZL-1, was isolated and identified from diseased Spodoptera litura larvae in soybean field. The pathogenicity antagonistic activity of FcS1GZL-1 against five insect pests were assessed, well its ability to colonize plants via root irrigation, induced resistance phytopathogens also measured. expression related genes analyzed using real-time RT-PCR. According results, strain could not only kill high inhibited growth vitro. Furthermore, repel pest feeding enhance through endophytic customization following which upregulated 12 jasmonic acid, salicylic ethylene, pathogen-related defense pathways roots. Herein, we first documented case naturally infecting insects, dual role controlling phytopathogens, promising biocontrol applications.

Language: Английский

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