Phages produce persisters
Laura Fernández‐García,
No information about this author
Joy Kirigo,
No information about this author
Daniel Huelgas‐Méndez
No information about this author
et al.
Microbial Biotechnology,
Journal Year:
2024,
Volume and Issue:
17(8)
Published: Aug. 1, 2024
Arguably,
the
greatest
threat
to
bacteria
is
phages.
It
often
assumed
that
those
escape
phage
infection
have
mutated
or
utilized
phage-defence
systems;
however,
another
possibility
a
subpopulation
forms
dormant
persister
state
in
manner
similar
demonstrated
for
bacterial
cells
undergoing
nutritive,
oxidative,
and
antibiotic
stress.
Persister
do
not
undergo
mutation
survive
lethal
conditions
by
ceasing
growth
transiently.
Slower
dormancy
play
key
physiological
role
as
they
allow
host
defence
systems
more
time
clear
infection.
Here,
we
investigated
how
lytic
isolating
surviving
from
plaques
of
T2,
T4,
lambda
(cI
mutant)
virulent
phages
sequencing
their
genomes.
We
found
can
attack
both
(i.e.
become
resistant)
without
persistent).
Specifically,
whereas
T4-resistant
lambda-resistant
with
over
100,000-fold
less
sensitivity
were
isolated
obvious
genetic
mutations
(e.g.
causing
mucoidy),
also
after
T2
no
significant
mutation,
retain
wild-type
sensitivity,
doses
antibiotics.
Corroborating
this,
adding
resulted
137,000-fold
survival
compared
addition
exponentially
growing
cells.
Furthermore,
our
results
seem
general
treatments
Klebsiella
pneumonia
Pseudomonas
aeruginosa
generated
Hence,
along
resistant
strains,
form
during
Language: Английский
Implications of lytic phage infections inducing persistence
Current Opinion in Microbiology,
Journal Year:
2024,
Volume and Issue:
79, P. 102482 - 102482
Published: May 6, 2024
Language: Английский
Mitomycin C as an Anti-Persister Strategy against Klebsiella pneumoniae: Toxicity and Synergy Studies
Antibiotics,
Journal Year:
2024,
Volume and Issue:
13(9), P. 815 - 815
Published: Aug. 28, 2024
The
combination
of
several
therapeutic
strategies
is
often
seen
as
a
good
way
to
decrease
resistance
rates,
since
bacteria
can
more
easily
overcome
single-drug
treatments
than
multi-drug
ones.
This
strategy
especially
attractive
when
targets
and
subpopulations
are
affected,
it
the
case
Klebsiella
pneumoniae
persister
cells,
subpopulation
able
transiently
survive
antibiotic
exposures.
work
aims
evaluate
potential
repurposed
anticancer
drug,
mitomycin
C,
combined
with
K.
lytic
phage
vB_KpnM-VAC13
in
vitro
its
safety
an
vivo
murine
model
against
two
clinical
isolates
this
pathogen,
one
them
exhibiting
imipenem-persister
phenotype.
At
same
time,
we
verified
absence
toxicity
C
at
concentration
using
human
chondrocyte
cell
line
T/C28a2.
viability
these
cells
was
checked
both
cytotoxicity
assays
flow
cytometry.
Language: Английский
Diverse physiological roles of the MqsR/MqsA toxin/antitoxin system
Deleted Journal,
Journal Year:
2024,
Volume and Issue:
1(1)
Published: Jan. 1, 2024
Abstract
There
is
tremendous
interest
in
the
use
of
bacteriophages
(phages)
to
combat
multi-drug
resistant
bacteria.
However,
implement
successfully
phage
therapy,
host
defense
systems
must
be
understood.
Toxin/antitoxins
(TAs)
are
most
prevalent
system,
and
MqsR/MqsA
TA
system
one
best-studied
systems.
This
phage-defense
was
discovered
a
whole-cell,
population-averaged,
transcriptome
study
designed
elucidate
biofilm-related
genes
Escherichia
coli
2004.
Biofilms
cells
cemented
themselves
or
surfaces.
Since
its
characterization
(as
April
2024),
has
been
utilized
over
1200
manuscripts,
although
role
cell
physiology
contested.
Here,
we
summarize
important
physiological
roles
this
including
(i)
general
stress
response
via
repression
rpoS,
(ii)
biofilm
formation
csgA,
(iii)
combating
bile
acid
gastrointestinal
tract
by
inhibiting
uptake
salt
deoxycholate,
(iv)
oxidative
based
on
single-cell
studies,
(v)
leading
persister
state.
Language: Английский
Phages Produce Persisters
Laura Fernández-García,
No information about this author
Joy Kirigo,
No information about this author
Daniel Huelgas‐Méndez
No information about this author
et al.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 17, 2023
SUMMARY
Bacteria
primarily
encounter
stress,
and,
arguably,
their
greatest
threats
are
phages.
It
is
often
assumed
that
those
bacteria
escape
phage
attack
have
mutated;
however,
another
possibility
a
subpopulation
forms
the
dormant
persister
state,
in
manner
similar
to
demonstrated
for
bacterial
cells
undergoing
nutritive,
oxidative,
and
antibiotic
stress.
Persister
do
not
undergo
mutation
survive
lethal
conditions
by
ceasing
growth
transiently.
Slower
dormancy
play
key
physiological
role
as
they
allow
host
defense
systems
more
time
clear
infection.
Here
we
investigated
how
lytic
infection
isolating
surviving
from
plaques
of
T2,
T4,
lambda
(cI
mutant)
virulent
We
found
can
both
(i.e.,
become
resistant)
without
persistent).
Specifically,
whereas
T4-resistant
lambda-resistant
with
over
100,000-fold
less
sensitivity
were
isolated
obvious
genetic
mutations
(e.g.,
causing
mucoidy),
also
after
T2
no
significant
mutation,
retain
wild-type
sensitivity,
doses
antibiotics.
Corroborating
this,
adding
resulted
137,000-fold
survival
compared
addition
exponentially-growing
cells.
Phage
treatments
Klebsiella
pneumonia
Pseudomonas
aeruginosa
generated
Hence,
along
resistant
strains,
form
during
Language: Английский
Mitomycin C as an Anti-persister Strategy Against Klebsiella Pneumoniae: Toxicity and Synergy Studies
Published: Aug. 6, 2024
The
combination
of
several
therapeutic
strategies
is
often
seen
as
a
good
way
to
decrease
the
resistance
rates,
since
bacteria
overcome
better
single-drug
treatments
than
multi-drug
ones.
This
strategy
especially
attractive
when
targets
and
subpopulations
are
affected,
it
case
Klebsiella
pneumoniae
persister
cells,
subpopulation
able
transiently
survive
antibiotic
exposures.
work
aims
evaluate
potential
repurposed
anticancer
drug,
mitomycin
C,
combined
with
K.
lytic
phage
vB_KpnM-VAC13
in
vitro
safety
an
vivo
murine
model
against
two
clinical
isolates
this
pathogen,
one
them
exhibiting
imipenem-persister
phenotype.
At
same
time,
we
verified
absence
toxicity
C
at
concentration
using
human
chondrocyte
cell
line
T/C28a2.
viability
these
cells
was
checked
both
by
cytotoxicity
assays
flow
cytometry.
Language: Английский