Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 10, 2025

Abstract Oleaginous yeasts are considered promising sources for lipid production due to their ability accumulate high levels of lipids under appropriate growth conditions. The current study aimed isolate and identify oleaginous having superior quantities lipids; enhancing using response surface methodology repeated-batch fermentation. Results revealed that, twenty marine were isolated, the most potent producer was Candida parapsilosis Y19 according qualitative screening test Nile-red dye. Orange peels used as substrate where C. produced 1.14 g/l at 23.0% in batch To enhance production, statistical optimization Taguchi design through Response carried out. Total increased 2.46 content 30.7% optimal conditions of: orange peel 75 g/l, peptone 7 yeast extract 5 inoculum size 2% ( v/v ), pH incubation period 6 d. Furthermore, fermentation enhanced total 4.19 folds (4.78 g/l) compared culture (before optimization). Also, 1.7 (39.1%) Fatty acid profile includes unsaturated fatty acids (USFAs) 74.8% saturated (SFAs) 25.1%. Additionally, fermentation, major oleic 45.0%; followed by linoleic 26.0%. In conclusion, is a strain production. both optimizations RSM efficient methods from Y19.

Language: Английский

---

One-pot bioconversion of fungal lipid to mycodiesel: a sustainable approach

Antonie van Leeuwenhoek, Journal Year: 2025, Volume and Issue: 118(4)

Published: March 15, 2025

Language: Английский

---

Untreated spent coffee grounds as an optimal feedstock for enhanced bioethanol production via simultaneous saccharification and fermentation

Biofuels Bioproducts and Biorefining, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Abstract This study investigated the potential of spent coffee grounds (SCG) as a sustainable feedstock for bioethanol production. Using simultaneous saccharification and fermentation (SSF), effects different SCG pretreatments on ethanol yield, reducing sugars consumption, yeast growth were compared. Surprisingly, untreated outperformed pretreated in all these aspects. Untreated achieved an yield 4.91 g L –1 , efficiency 81.11%, 1.86 × 10 7 cells/mL. These values significantly higher than by various methods. contained levels inhibitory compounds (polyphenols, furfurals, hydroxymethylfurfural) but also exhibited enhanced growth. unexpected finding suggests positive interaction between components metabolism. Polyphenols free fatty acids had effect correlated strongly with (FFA), suggesting protective response to oxidative stress caused certain polyphenols, thereby enhancing Our findings demonstrate untapped economically viable approach offers valuable alternative traditional feedstocks, contributing circular economy reliance fossil fuels.

Language: Английский

---

Transcriptome analysis revealed the molecular mechanism of increasing the yield of medium-chain fatty acid ethyl esters through redox potential regulation driven by hydrogen peroxide during simulated wine alcoholic fermentation

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106550 - 106550

Published: April 1, 2025

Language: Английский

---

Reprogramming protein stability in Escherichia coli to improve four-carbon dicarboxylic acids production

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 493, P. 152893 - 152893

Published: June 6, 2024

Language: Английский

---

Transcriptional and Post-Translational Roles of Calcineurin in Cationic Stress and Glycerol Biosynthesis in Cryptococcus neoformans

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(8), P. 531 - 531

Published: July 30, 2024

Stress management is an adaptive advantage for survival in adverse environments. Pathogens face this challenge during host colonization, requiring appropriate stress response to establish infection. The fungal pathogen Cryptococcus neoformans undergoes thermal, oxidative, and osmotic stresses the environment animal host. Signaling systems controlled by Ras1, Hog1, calcineurin respond high temperatures stress. Cationic caused Na+, K+, Li+ can be overcome with glycerol, preferred osmolyte. Deleting glycerol phosphate phosphatase gene (GPP2) prevents cells from accumulating due a block last step of its biosynthetic pathway. Gpp2 accumulates phosphorylated form cna1Δ strain, physical interaction between Cna1 was found; moreover, gpp2Δ strain slow growth has attenuated virulence models We provide biochemical evidence that 1 M NaCl increases content wild type, whereas gpp2Δ, cna1Δ, cnb1Δ mutants fail accumulate it. deletion or renders yeast sensitive cationic stress, Gfp-Gpp2 protein assumes abnormal localization. suggest mechanism which controls at post-translational level, affecting localization activity, leading biosynthesis. Also, we showed transcriptional profile glycerol-deficient established mediated calcineurin; among biological processes differentially expressed are carbon utilization, translation, transmembrane transport, glutathione metabolism, oxidative response, transcription regulation. To our knowledge, first time been described. These results have implications adaptability.

Language: Английский

---

A Rewired NADPH-Dependent Redox Shuttle for Testing Peroxisomal Compartmentalization of Synthetic Metabolic Pathways in Komagataella phaffii

Microorganisms, Journal Year: 2024, Volume and Issue: 13(1), P. 46 - 46

Published: Dec. 30, 2024

The introduction of heterologous pathways into microbial cell compartments offers several potential advantages, including increasing enzyme concentrations and reducing competition with native pathways, making this approach attractive for producing complex metabolites like fatty acids alcohols. However, measuring subcellular these remains technically challenging. Here, we explored 3-hydroxypropionic acid (3-HP), readily quantifiable sharing the same precursors—acetyl-CoA, NADPH, ATP—with above-mentioned products, as a reporter metabolite peroxisomal engineering in yeast Komagataella phaffii. To end, malonyl-CoA reductase pathway 3-HP production was targeted peroxisome K. phaffii using PTS1-tagging system, further tested different carbon sources. Thereafter, used compartmentalized system to showcase impact strategies aimed at enhancing NADPH pool. Co-overexpression genes encoding NADPH-dependent redox shuttle from Saccharomyces cerevisiae (IDP2/IDP3) significantly increased yields across all substrates, whereas targeting S. NADH kinase Pos5 failed improve production. This study highlights biosensor evaluating acetyl-CoA NAPDH availability by simply quantifying 3-HP, demonstrating its peroxisome-based metabolic yeast.

Language: Английский

---

Comparative Genomic Analysis Reveals Key Changes in the Genome of Acremonium chrysogenum That Occurred During Classical Strain Improvement for Production of Antibiotic Cephalosporin C

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 181 - 181

Published: Dec. 28, 2024

From the 1950s to present, main tool for obtaining fungal industrial producers of secondary metabolites remains so-called classical strain improvement (CSI) methods associated with multi-round random mutagenesis and screening level target products. As a result application such techniques, yield in high-yielding (HY) strains was increased hundreds times compared wild-type (WT) parental strains. However, events that occur at molecular during CSI programs are still unknown. In this paper, an attempt made identify characteristic changes genome occurred

Language: Английский

---