Current Microbiology, Journal Year: 2024, Volume and Issue: 81(12)
Published: Nov. 7, 2024
Language: Английский
Published: Jan. 1, 2025
Language: Английский
Citations
1
Animals, Journal Year: 2025, Volume and Issue: 15(6), P. 798 - 798
Published: March 11, 2025
Bacillus spp. have emerged as pivotal sources of probiotic preparations, garnering considerable attention in recent years owing to their vigorous bacteriostatic activity and antimicrobial resistance. This study aimed investigate these characteristics depth verify the safety velezensis K12, a strain isolated from broiler intestine. The K12 was identified based on its morphology 16S rDNA sequence homology analysis. Subsequently, B. evaluated for acid resistance, bile salt gastrointestinal tolerance, drug sensitivity, activity. Additionally, whole-genome sequencing technology employed dissect genomic components further, aiming explore potential applications strain. sensitive six antibiotics had tolerance. Furthermore, it showed potent against wide range pathogenic bacteria, including Escherichia coli (E. coli), Staphylococcus aureus, Salmonella, Clostridium perfringens, cereus, Vibrio parahaemolyticus. complete genome revealed length 3,973,105 base pairs containing 4123 coding genes, among which 3973 genes were functionally annotated. analysis associated with adhesion, antioxidants, secondary metabolite production, whereas no functional related enterotoxins or transferable antibiotic resistance detected, thereby confirming properties K12. exhibits broad-spectrum vitro safety, positioning candidate developing preparations.
Language: Английский
Citations
0
Ars Pharmaceutica (Internet), Journal Year: 2025, Volume and Issue: 66(2), P. 152 - 173
Published: March 19, 2025
Introduction: The current investigation assessed a novel strain of Bacillus velezensis SNR14-4, isolated from the gills Nile tilapia intending to consider it as promising probiotic contender. Methods: Initially, an extensive analysis genome particular isolate was carried out employing bioinformatics tools anticipate its characteristics and potential attributes. total recognized B. via 16S rRNA whole-genome sequencing phylogenetic analysis, is composed singular circular chromosome with size 4.1 Mb, length 4183910 bp, average guanine-cytosine (GC) content 46.52 %. Valuable insights were acquired utilizing AntiSMASH detect secondary metabolite biosynthetic gene clusters, functional annotation relevant traits accomplished by RASTtk PROKKA. absence virulence elements, ascertained genomic facilitated targeted in vitro exploration. Results: SNR14-4 displayed notable exhibited antimicrobial efficacy against common fish pathogens. HR-LCMS QTOF microbial extract unveiled several potent compounds synthesized strain. Conclusions: showcases promise candidate, either single point source or part consortia made similar strains.
Language: Английский
Citations
0
Horticulturae, Journal Year: 2025, Volume and Issue: 11(4), P. 384 - 384
Published: April 4, 2025
The endophytic strain Amfr20 was isolated from roots of the olive tree var. Amfissa. Based on core-genome phylogenomic analyses, it classified as Bacillus velezensis. isolate showed positive results in numerous plant growth promoting traits, well abiotic stress tolerance and colonization related traits vitro. Furthermore, exhibited antifungal activity vitro through diffusible volatile compounds. Whole genome analysis revealed that possesses large various arsenals secondary metabolite biosynthetic gene clusters involved bioagent’s functional properties, including promotion, colonization, defense elicitation, having genomic potential for mediation. TLC-bioautography, ethyl acetate extracts secreted agar-diffusible compounds single dual cultures were found to be bioactive independently fungal pathogen’s interaction. bacterial endophyte also proved efficient suppressing severity anthracnose rot gray mold post-harvest diseases fruits table grape berries, respectively. Lastly, beneficially affected Arabidopsis thaliana under normal saline conditions, while boosting development Solanum lycopersicum seed biopriming root irrigation methods. this multilevel study indicate novel velezensis is a promising bioagent should exploited future an ecological biopesticide and/or biostimulant.
Language: Английский
Citations
0
Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16
Published: April 9, 2025
Crested ibis (Nipponia nippon) is a rare bird whose intestinal tract rich in lactic acid bacteria (LAB), but there less research on LAB isolated from crested ibises. From the fecal samples, Twenty isolates were obtained samples and subjected to series of tests, including biochemical identification, bile tolerance assays, vitro pathogen inhibition, cell surface hydrophobicity assessment, antibiotic susceptibility testing, hemolytic activity evaluation determine their probiotic potential. We fed L. plantarum E7 mice evaluate safety. Nanopore PromethION48 Illumina Novaseq sequencing platforms used sequence genome E7. Five (D1, D2, D6, D8) able survive under low high salt conditions. Except for D8, other four D6 E7) exhibited inhibitory against tested pathogens. Strain displayed least resistance antibiotics, only showed medium hydrophobicity. Further characterization identified strain as Lactiplantibacillus (L. plantarum) through 16S rDNA sequencing. did not observe adverse effects growth performance, blood composition mice. consists circular chromosome two plasmids. The encodes 3024 genes that associated with adhesion, tolerance, antioxidant enzymes, well production secondary metabolites. In contrast, plasmids contain fewer coding genes. Functional annotation via KEGG GO database analysis indicated are primarily involved carbohydrate metabolism, amino vitamin cofactor biological process, molecular function. This study provides theoretical foundation developing new
Language: Английский
Citations
0
BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)
Published: April 18, 2025
Abstract Background Healthcare-associated infections (HAIs) caused by microbes that acquire antimicrobial resistance (AMR) represent an increasing threat to human health worldwide. The high use of chemical disinfectants aimed at reducing the presence pathogens in hospital environment can simultaneously favor selection resistant strains, potentially worsening AMR concerns. In search for sustainable ways control bioburden without affecting this aspect, probiotic-based sanitation (PBS) using Bacillus spp. was proposed achieve stable reduction pathogens, AMR, and associated HAIs. Although probiotics are classified as nonpathogenic, comprehensive data about potential genetic alterations these following prolonged contact with surrounding not yet available. This study assess depth content PBS- isolates evaluate any eventual variations occurred during their usage. Results WGS analysis used precise identification species detailed profiling SNPs, resistome, virulome, mobilome. Analyses were conducted on both original PBS detergent 172 environmental from eight hospitals sanitized over a 30-month period. two B. subtilis velezensis identified product environment, SNP revealed clusters each species. No virulence/resistance genes or mobile conjugative plasmids detected either strain analyzed isolates, confirming stability low/no tendency be involved horizontal gene transfer events. Conclusions obtained metagenomic absence sequences lack all analyzed, despite continuous pathogens. These results support safety analyzed. Further studies whole genomes other , possibly longer periods under stress conditions, would interest since they may provide further confirmation safety.
Language: Английский
Citations
0
PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0319345 - e0319345
Published: April 24, 2025
Extreme environments like arid and semi-arid deserts harbor unique microbial diversity, offering rich sources of specialized metabolites. This study explores Bacillus velezensis RVMD2, a strain isolated from rock varnish in the Ma’an Desert, Jordan. The genome was sequenced using Illumina NextSeq 2000 platform, resulting 4,212,579 bp assembly with GC content 45.94%. assembled comprises 112 contigs encodes 4,250 proteins, 77 tRNA genes, 4 rRNA genes. Phylogenetic analysis 16S gene indicated 99.84% similarity to previously identified B. strains. Whole-genome phylogeny EzBiome, MiGA, TYGS confirmed its classification as . Functional annotation genes involved carbohydrate metabolism, including 324 carbohydrate-active enzyme (CAZyme) stress response, secondary metabolite biosynthesis. also contains 50 associated heavy metal resistance plant growth promotion. Analysis AntiSMASH 12 biosynthetic clusters production metabolites, fengycin, surfactin, polyketides, terpenes, bacteriocins. Notably, several did not match any known sequences, suggesting presence potentially novel antimicrobial compounds. genomic features RVMD2 highlight adaptability extreme potential for biotechnological applications, bioremediation discovery bioactive
Language: Английский
Citations
0
Microbial Ecology, Journal Year: 2025, Volume and Issue: 88(1)
Published: May 1, 2025
Language: Английский
Citations
0
Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15
Published: May 9, 2024
The white-spotted flower chafer (
Language: Английский
Citations
3
Marine Pollution Bulletin, Journal Year: 2024, Volume and Issue: 209, P. 117115 - 117115
Published: Oct. 22, 2024
Language: Английский
Citations
3