Emergence of highly virulent multidrug and extensively drug resistant Escherichia coli and Klebsiella pneumoniae in buffalo subclinical mastitis cases

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 5, 2025

This study aimed to characterize virulence and antibiotic resistance genes in multidrug-resistant (MDR) extensively drug-resistant (XDR) Escherichia coli Klebsiella pneumoniae isolated from cases of subclinical mastitis (SCM) buffaloes. A cross-sectional was conducted on 1540 quarter milk samples collected 385 Milk were screened using the California Mastitis Test Modified Whiteside Test. Positive underwent bacterial culture, biochemical tests, biofilm detection molecular analysis for pathogen identification virulence, resistance, extended-spectrum beta-lactamase (ESBL) genes. The prevalence SCM 67.9% (1046/1540) at level 80.8% (311/385) animal level. E. identified 9.5% (146/1540) samples, while K. detected 9.09% (140/1540). Virulence genes, such as stx1 (27.4%), including aac(3)-iv (77.4%) tetA (76.7%), exhibited higher prevalence. Additionally, β-lactamase notably blaTEM (67.1%), ESBL blaCTX-M1, detected. Biofilm formation 83.6% (122/146) isolates 75.7% (106/140) isolates. Antimicrobial susceptibility testing revealed significant ampicillin, amoxicillin-clavulanic acid, aminoglycosides. MDR observed 31.5% 39.3% isolates, with XDR rates 8.9% 12.9%, respectively. These findings underscore alarming spread resistant pathogens SCM-affected buffaloes, emphasizing urgent need ongoing surveillance targeted intervention strategies.

Language: Английский

Antibiotic resistance and virulence patterns of O25 and O16 serogroups in uropathogenic Escherichia coli

BMC Research Notes, Journal Year: 2025, Volume and Issue: 18(1)

Published: April 10, 2025

Language: Английский