Circbank: a comprehensive database for circRNA with standard nomenclature

RNA Biology, Journal Year: 2019, Volume and Issue: 16(7), P. 899 - 905

Published: April 25, 2019

Circular RNAs (circRNAs) represent a new type of regulatory RNA which forms covalently closed continuous loop from back-splicing events, process in the downstream 5' splice site and 3' are linked. Emerging evidence indicates that circRNAs exert layer transcriptional post-transcriptional regulation gene expression. However, there is no standard nomenclature circRNA, although study has exploded past few years. Here we present circbank ( www.circbank.cn ), comprehensive database for human circRNAs, where novel naming system based on host genes was implemented. In addition to system, collected other five features including miRNA binding site, conservation m6A modification mutation protein-coding potential circRNAs. Circbank publicly available allows users query, browse download with all six provided, different search criteria. The may serve as resource facilitate research function

Language: Английский

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Molecular roles and function of circular RNAs in eukaryotic cells

Cellular and Molecular Life Sciences, Journal Year: 2017, Volume and Issue: 75(6), P. 1071 - 1098

Published: Nov. 7, 2017

Protein-coding and noncoding genes in eukaryotes are typically expressed as linear messenger RNAs, with exons arranged colinearly to their genomic order. Recent advances sequencing mapping RNA reads reference genomes have revealed that thousands of express also covalently closed circular RNAs. Many these circRNAs stable contain exons, but not translated into proteins. Here, we review the emerging understanding both, produced by co- posttranscriptional head-to-tail "backsplicing" a downstream splice donor more upstream acceptor, well generated from intronic lariats during colinear splicing, may exhibit physiologically relevant regulatory functions eukaryotes. We describe how impact gene expression host locus affecting transcriptional initiation elongation or they partake controlling function other molecules, for example interacting microRNAs conclude an outlook circRNA dysregulation affects disease, stability might be exploited biomedical applications.

Language: Английский

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Cisplatin nephrotoxicity: new insights and therapeutic implications

Nature Reviews Nephrology, Journal Year: 2022, Volume and Issue: 19(1), P. 53 - 72

Published: Oct. 13, 2022

Language: Английский

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CircRNA hsa_circRNA_104348 promotes hepatocellular carcinoma progression through modulating miR-187-3p/RTKN2 axis and activating Wnt/β-catenin pathway

Cell Death and Disease, Journal Year: 2020, Volume and Issue: 11(12)

Published: Dec. 14, 2020

Abstract Circular RNAs (circRNAs) have confirmed to participate in diverse biological functions cancer. However, the expression patterns of circRNAs on hepatocellular carcinoma (HCC) remains unclear. In present study, we clarified that hsa_circRNA_104348 was dramatically upregulated HCC tissues and cells. Patients with displaying high level possessed poor prognosis. Has_circ_104348 facilitated proliferation, migration, invasion, meanwhile suppressed apoptosis cell. Furthermore, directly targeted miR-187–3p, could regulate miR-187-3p affect cells, may effect Wnt/β-catenin signaling pathway. Moreover, RTKN2 be a direct target miR-187-3p. addition, knockdown attenuated tumorigenesis lung metastasis vivo. Taken together, these findings indicated circular RNA might function as competing endogenous (ceRNA) promotes progression by targeting miR-187–3p/RTKN2 axis activating

Language: Английский

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Three isoforms of exosomal circPTGR1 promote hepatocellular carcinoma metastasis via the miR449a–MET pathway

EBioMedicine, Journal Year: 2019, Volume and Issue: 40, P. 432 - 445

Published: Jan. 8, 2019

BackgroundThe role of exosomal circular RNAs (circRNAs) in Hepatocellular carcinoma (HCC) cells with high metastatic potential has been little studied.MethodsExosomal circRNA from non-metastatic (HepG2), low (97L), and (LM3) were sequencing. Metastatic-related circRNAs serum HCC patients measured their association clinical prognosis was evaluated. Furthermore, candidate functional LM3-derived exosomes assessed.FindingsLM3 enhanced the cell migration invasion HepG2 97 L cells. CircPTGR1, a three isoforms, specifically expressed LM3 cells, upregulated associated stage prognosis. Knockdown circPTGR1 expression suppressed 97L induced by co-culturing exosomes. Bioinformatics, co-expression analysis, luciferase assay indicated that competed MET to target miR449a.InterpretationHigher can confer this on those lower or no via circPTGR1, resulting increased migratory invasive abilities cells.FundNational Natural Science Foundation China (No. 81470870, 81670601, 81570593), Guangdong 2015A030312013, 2015A030313038), Sci-tech Research Development Program Province (2014B020228003), Guangzhou City 201508020262, 201400000001-3, 201604020001, 201607010024), Innovative Funds for Small Medium-Sized Enterprises (2016A010119103), Pearl River S&T Nova (201710010178), National 13th Five-Year Technology Plan Major Projects 2017ZX10203205-006-001).

Language: Английский

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Circular RNA hsa_circ_0003575 regulates oxLDL induced vascular endothelial cells proliferation and angiogenesis

Biomedicine & Pharmacotherapy, Journal Year: 2017, Volume and Issue: 95, P. 1514 - 1519

Published: Sept. 21, 2017

Language: Английский

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Circular RNAs: a new frontier for cancer diagnosis and therapy

Journal of Hematology & Oncology, Journal Year: 2018, Volume and Issue: 11(1)

Published: Feb. 13, 2018

In recent years, circular RNAs (circRNAs) have attracted considerable attention because they play a significant role in many fields of cancer biology. Additionally, it has become increasingly clear that circRNAs the potential to make contributions successful application individualized medicine. This brief review introduces by describing their as biomarker and therapeutic target discussing possible strategies them. also presents challenges are encountered for definitive entry into clinical practice. Clearly, our understanding helps add new dimension molecular structure will provide opportunities treatment.

Language: Английский

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The RNA Methyltransferase NSUN2 and Its Potential Roles in Cancer

Cells, Journal Year: 2020, Volume and Issue: 9(8), P. 1758 - 1758

Published: July 22, 2020

5-methylcytosine is often associated as an epigenetic modifier in DNA. However, it also found increasingly a plethora of RNA species, predominantly transfer RNAs, but cytoplasmic and mitochondrial ribosomal enhancer number long noncoding RNAs. Moreover, this modification can be messenger RNAs has led to increasing appreciation that methylation functionally regulate gene expression cellular activities. In mammalian cells, the addition m5C cytosines carried out by enzymes NOL1/NOP2/SUN domain (NSUN) family well DNA methyltransferase homologue DNMT2. regard, NSUN2 critical for adding mRNA. review, using non-small cell lung cancer other cancers primary examples, we discuss recent developments known functions its potential role cancer.

Language: Английский

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Functions and clinical significance of circular RNAs in glioma

Molecular Cancer, Journal Year: 2020, Volume and Issue: 19(1)

Published: Feb. 15, 2020

Abstract CircRNAs are a class of single-stranded RNA molecules with covalently closed loop structure and have been characterized by high stability, abundance, conservation, display tissue/developmental stage-specific expression, furthermore, based on the abundance in distinct body fluids or exosomes, circRNAs present novel biomarkers targets for diagnosis prognosis cancers. Recently, regulatory mechanisms biogenesis molecular functions, including miRNAs RBPs sponge, translation as well transcriptional splicing regulation, gradually uncovered, although various aspects remained to be elucidated combination deep-sequence bioinformatics. Accumulating studies indicated that more enriched neuronal tissues partly due specific genes promoting circularization, suggesting dysregulation is closely related diseases nervous system, glioma. In this review, we elaborate biogenesis, databases advances especially involved pathways, highlight its great value diagnostic therapeutic

Language: Английский

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circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells

Nucleic Acids Research, Journal Year: 2019, Volume and Issue: 47(7), P. 3580 - 3593

Published: Feb. 20, 2019

NF-κB-mediated inflammatory phenotypic switching of vascular smooth muscle cells (VSMCs) plays a central role in atherosclerosis and neointimal formation. However, little is known about the roles circRNAs regulation NF-κB signaling. Here, we identify involvement circ-Sirt1 that was one transcripts SIRT1 host gene VSMC response hyperplasia. First, cytoplasm, directly interacts with sequesters p65 from nuclear translocation induced by TNF-α sequence-dependent manner. The inhibitory complex circ-Sirt1-NF-κB not dependent on IκBα. Second, binds to miR-132/212 interferes mRNA, facilitates expression SIRT1. Increased results deacetylation inactivation p65. These findings illustrate novel non-coding RNA regulator phenotype.

Language: Английский

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