Progression in the study of protective effect of microRNAs against sevoflurane-induced postoperative cognitive dysfunction and potential mechanisms DOI Creative Commons
Jun Chen,

Lin-Yan Li,

Bo-Ya Zhang

et al.

Turkish Journal of Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

Abstract As one of the volatile anaesthetic drugs commonly used in clinical anaesthesia, sevoflurane is extensively general anaesthesia surgery because its rapid induction, hemodynamic stability, and metabolism. However, postoperative cognitive dysfunction (POCD) most prevalent complications application. Neuroinflammation neuronal apoptosis are currently believed to be primary mechanisms responsible for development dysfunction. MicroRNAs (miRNAs) can negatively regulate expression target genes, thereby influencing neurological processes. Therefore, effects potential miRNAs on function may provide new ideas prevention treatment sevoflurane-induced POCD, a topic intense research interest at moment. The involved regulation by sevoflurane, genes downstream signalling pathways were reviewed order obtain comprehensive understanding function.

Language: Английский

The role of epigenetic modification in postoperative cognitive dysfunction DOI

Wei-Feng Wu,

Jia-Tao Lin,

Yongkang Qiu

et al.

Ageing Research Reviews, Journal Year: 2023, Volume and Issue: 89, P. 101983 - 101983

Published: June 13, 2023

Language: Английский

Citations

16
A crosstalk between circular RNA, microRNA, and messenger RNA in the development of various brain cognitive disorders DOI Creative Commons
Liang He, Furong Zhang,

Yuling Zhu

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2022, Volume and Issue: 15

Published: Oct. 18, 2022

Patients with Alzheimer's disease (AD), Parkinson's (PD), traumatic brain injury (TBI), stroke, and postoperative neurocognitive disorder (POND) are commonly faced disorders limited therapeutic options. Some non-coding ribonucleic acids (ncRNAs) involved in the development of various cognitive disorders. Circular RNAs (circRNAs), a typical group ncRNAs, can function as competitive endogenous (ceRNAs) to dysregulate shared microRNAs (miRNAs) at post-transcription level, inhibiting regulation miRNAs on their targeted messenger (mRNAs). circRNAs abundant central nervous system (CNS) diseases cause disorders, but exact roles unclear. The crosstalk between circRNA, miRNA, mRNA plays an important role pathogenesis these dysfunction abnormal conditions including AD, PD, TBI, POND. In this review, we summarized participation circRNA neuroglial damage inflammation. Finally, aimed highlight regulatory mechanisms circRNA–miRNA–mRNA networks provide new insights into therapeutics diseases.

Language: Английский

Citations

20
Impaired hippocampal neurogenesis associated with regulatory ceRNA network in a mouse model of postoperative cognitive dysfunction DOI Creative Commons
Jingrun Lin,

Xiaoqiu Zhu,

Xuan Li

et al.

BMC Anesthesiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 6, 2025

Postoperative cognitive dysfunction (POCD) represents a post-surgical complication that features progressive impairment and memory loss, often occurring in elderly patients. This study aimed to investigate the potential biological mechanisms underlying POCD. Male C57BL/6 mice (2 17 months old) were randomly assigned surgery or control groups. The group underwent laparotomy under 1.5% isoflurane anesthesia, while controls received no intervention. Cognitive function was assessed 7–10 days post-surgery using open field, Y-maze, novel object recognition tests. Hippocampal mRNA expression analyzed Encyclopedia of Genes Genomes (KEGG) Gene Ontology (GO) enrichment. A competing endogenous RNA (ceRNA) network constructed microRNA (miRNA) target prediction databases (miRanda, miRTarbase, miRcode) sequencing results. Key findings validated by RT-qPCR immunofluorescence. Connectivity Map (CMap) database queried predict POCD treatments. Aging significantly affected mice's spontaneous activity field test (F1, 28 = 8.933, P < 0.01) proportion time spent center area 5.387, 0.05). Surgery reduced rate alternations Y-maze 16.94, 0.001) index 6.839, 0.05) aging mice, but had effect on young mice. Transcriptome analysis revealed downregulated multiple neurogenesis-related genes hippocampus. Doublecortin (DCX) immunofluorescence staining confirmed hippocampal neurogenesis which further decreased after surgery. We identified several key lncRNAs miRNAs implicated regulation. Additionally, drugs predicted as therapeutic candidates for treatment. Both have complex effects transcriptome significant decrease may be reason increased susceptibility regulatory lncRNAs, miRNAs, provide targets prevention

Language: Английский

Citations

0
CircAKT3 alleviates postoperative cognitive dysfunction by stabilizing the feedback cycle of miR-106a-5p/HDAC4/MEF2C axis in hippocampi of aged mice DOI Creative Commons
Xuan Wang,

Xiaole Tang,

Pengfei Zhu

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: March 13, 2024

Abstract Circular RNAs (circRNAs) have garnered significant attention in the field of neurodegenerative diseases including Alzheimer’s due to their covalently closed loop structure. However, involvement circRNAs postoperative cognitive dysfunction (POCD) is still largely unexplored. To identify genes differentially expressed between non-POCD (NPOCD) and POCD mice, we conducted whole transcriptome sequencing initially this study. According expression profiles, observed that circAKT3 was associated with hippocampal neuronal apoptosis mice. Moreover, found overexpression reduced neurons alleviated POCD. Subsequently, through bioinformatics analysis, our data showed vitro vivo elevated abundance miR-106a-5p significantly, resulting a decrease HDAC4 protein an increase MEF2C protein. Additionally, effect blocked by inhibitor. Interestingly, could activate transcription promoter form positive feedback loop. Therefore, findings revealed more potential modulation ways circRNA-miRNA miRNA-mRNA, providing different directions targets for preclinical studies

Language: Английский

Citations

3
The NF-κB Pathway: Key Players in neurocognitive functions and related disorders DOI Creative Commons

Danfeng Yang,

Junwei Su,

Yeru Chen

et al.

European Journal of Pharmacology, Journal Year: 2024, Volume and Issue: 984, P. 177038 - 177038

Published: Oct. 5, 2024

Language: Английский

Citations

2
Decoding competitive endogenous RNA regulatory network in postoperative cognitive dysfunction DOI Creative Commons
Wei Wang, Pengwei Huo, Lei Zhang

et al.

Frontiers in Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: Sept. 20, 2022

Postoperative cognitive dysfunction (POCD) is a common postoperative neurological complication in elderly patients. Circular RNAs (circRNAs) are abundant the mammalian brain and can probably regulate function. However, competitive endogenous RNA (ceRNA) regulatory network POCD remains illiterate. Transcriptomic signatures hippocampus of mice derived from Gene Expression Omnibus (GEO) dataset GSE190880, GSE95070, GSE115440 were used to identify circRNA, miRNA, mRNA expression profiles compared with controls, respectively. A set differentially expressed RNAs, including 119 circRNAs, 33 miRNAs, 49 mRNAs identified. Transcript validation showed enhanced circ_0001634, circ_0001345, circ_0001493. ceRNA composed three six was established. The hub further found be involved hormone catabolic process regulation canonical Wnt signaling pathway, revealing their crucial role POCD. Finally, miRNAs four verified by qRT-PCR. These results based on bioinformatics PCR array suggest that circ_0001634/miR-490-5p/Rbm47, circ_0001634/miR-490-5p/Sostdc1, circ_0001634/miR-7001-5p/Sostdc1, circ_0001345/miR-7001-5p/Sostdc1, circ_0001493/miR-7001-5p/Sostdc1 may novel diagnostic biomarkers therapeutic targets for

Language: Английский

Citations

12
Forensic age estimation from human blood using age-related microRNAs and circular RNAs markers DOI Creative Commons
Junyan Wang, Haixia Zhang, Chunyan Wang

et al.

Frontiers in Genetics, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 22, 2022

Aging is a complicated process characterized by progressive and extensive changes in physiological homeostasis at the organismal, tissue, cellular levels. In modern society, age estimation essential large variety of legal rights duties. Accumulating evidence suggests roles for microRNAs (miRNAs) circular RNAs (circRNAs) regulating numerous processes during aging. Here, we performed circRNA sequencing two groups analyzed microarray data 171 healthy subjects (17-104 years old) downloaded from Gene Expression Omnibus (GEO) ArrayExpress databases with integrated bioinformatics methods. A total 1,403 were differentially expressed between young old groups, 141 exclusively elderly samples while 10 only subjects. Based on their expression pattern these categorized into three classes: age-related old, age-limited expression-young only, expression-old only. Top five among classes 18 screened online selected to validate using RT-qPCR tests. An independent set 200 blood (20-80 was used develop prediction models based 15 noncoding (11 4 RNAs). Different machine learning algorithms applied, including regression tree, bagging, support vector (SVR), random forest (RFR), XGBoost. Among them, model best both training (mean absolute error = 3.68 years, r 0.96) testing (MAE 6.840 0.77). Models one single type predictors, RNAs-only or microRNAs-only, result bigger errors. Smaller errors shown males than females when constructing according different-sex separately. Putative microRNA targets (430 genes) enriched senescence pathway cell differentiation regulation, indirectly indicating that our study correlated development This demonstrates RNA aging clock has potential predicting chronological will be an available biological marker routine forensic investigation predict samples.

Language: Английский

Citations

12
Obese mice induced by high-fat diet have differential expression of circular RNAs involved in endoplasmic reticulum stress and neuronal synaptic plasticity of hippocampus leading to obesity-associated cognitive impairment DOI Creative Commons

Yan Niu,

Pan Chang,

Tian Liu

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2022, Volume and Issue: 15

Published: Oct. 3, 2022

Obesity induced by a high-fat diet (HFD) is an important cause of impaired memory and cognitive function, but the underlying mechanisms are not clear. In present study, we analyzed levels circRNAs in hippocampus C57BL/6J mice evaluated cognition ability with HFD using Morris water maze Y-maze approaches to explore potential linking obesity-associated impairment. Learning performance showed that HFD-induced obesity have cognition. The Arraystar analysis displayed leads differential expression (DE-circRNAs) mice. total, 46 circular RNAs elevated 10 decreased were identified. Among them, mmu_circRNA_004797 was identified be significantly downregulated mmu_circRNA_21040 upregulated HFD-fed mice, compared control PCR test. Bioinformatics also related neuronal function behavior, material transport process, while participated process cell response external stimuli, such as cellular nutrient levels. Furthermore, KEGG pathway mainly involved Axon guidance, calcium signaling pathway, ErbB pathway. Only single significant is, "protein processing endoplasmic reticulum", observed circRNAs. Finally, examined deficits hippocampal synaptic plasticity detected ER stress-related protein. results stress activated hippocampus, displayed. Our demonstrated most likely implicated predisposition

Language: Английский

Citations

10
Construction and analysis of circular RNA-associated competing endogenous RNA network in the hippocampus of aged mice for the occurrence of postoperative cognitive dysfunction DOI Creative Commons
Mingzhu Zhang, Zizheng Suo, Yinyin Qu

et al.

Frontiers in Aging Neuroscience, Journal Year: 2023, Volume and Issue: 15

Published: March 27, 2023

Circular RNAs are highly stable single-stranded circular and enriched in the brain. Previous studies showed that circRNAs, as part of competing endogenous (ceRNAs) network, play an important role neurodegenerative psychiatric diseases. However, mechanism circRNA-related ceRNA networks postoperative cognitive dysfunction (POCD) has not been elucidated yet. POCD usually occurs elderly patients is characterized by hippocampal dysfunction. Here, aged C57BL/6 mice were subjected to exploratory laparotomy under sevoflurane anesthesia, this model was verified Morris water maze test. Whole-transcriptome sequencing performed on hippocampus control group (Con) surgery group. One hundred seventy-seven DEcircRNAs, 221 DEmiRNAs 2,052 DEmRNAs identified between two groups. A network established with 92 DEcircRNAs having binding sites 76 549 target DEmRNAs. In functional enrichment analysis, a pathological pattern highlighted network: Abnormal metabolic process neural cells, including oxygen metabolism, could promote apoptosis then affect synaptic function, which may undermine plasticity eventually lead changes function other behavioral patterns. conclusion, specific circRNAs-miRNAs-mRNAs provided novel insights into regulatory mechanisms revealed potential therapeutic gene targets.

Language: Английский

Citations

5
Integration of ATAC-Seq and RNA-Seq identifies key genes and pathways involved in the neuroprotection of S-adenosylmethionine against perioperative neurocognitive disorder DOI Creative Commons
Feifei Xu, Peilin Cong, Zhihong Lu

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2023, Volume and Issue: 21, P. 1942 - 1954

Published: Jan. 1, 2023

The gene-regulatory landscape is highly dynamic in healthy and diseased brains. DNA methylation a well-known epigenetic modification that regulates gene expression, our previous study demonstrated S-adenosylmethionine (SAM), methylome modulator, was neuroprotectant against perioperative neurocognitive disorder (PND). However, the underlying mechanism remains to be elucidated. Here, we integrated an assay for transposase-accessible chromatin by sequencing (ATAC-seq) RNA (RNA-seq) identify key genes pathways involved neuroprotection of SAM PND. Our RNA-seq data biological processes such as Wnt signaling, inflammatory response, transcription long-term potentiation likely mediate SAM. ATAC-seq provided comprehensive maps accessibility changes induced laparotomy + treatment, functional annotation regions with high variations highlighted role signaling pathway PND pathogenesis treatment. Further motif analysis identified factors (e.g., CTCF, TFDP1, TCFL5, KLF15, ZBTB14, TFAP2E) may participate In conclusion, current provides epigenomic perspective understand its treatment

Language: Английский

Citations

4