Neuropeptide Modulation of Bidirectional Internetwork Synapses DOI Creative Commons
Savanna-Rae H. Fahoum, Dawn M. Blitz

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 16, 2023

Abstract Oscillatory networks underlying rhythmic motor behaviors, and sensory complex neural processing, are flexible, even in their neuronal composition. Neuromodulatory inputs enable neurons to switch participation between networks, or participate multiple simultaneously. Neuromodulation of internetwork synapses can both recruit coordinate a switching neuron second network. We previously identified an example which is recruited into dual-network activity via peptidergic modulation intrinsic properties. now ask whether the same neuropeptide also modulates for coordination. The crab ( Cancer borealis ) stomatogastric nervous system contains two well-defined feeding-related (pyloric, food filtering, ∼1 Hz; gastric mill, chewing, ∼0.1 Hz). projection MCN5 uses Gly 1 -SIFamide pyloric-only LPG dual pyloric plus mill-timed bursting LPG’s Descending input not required coordinated rhythm, thus intra-network its network must underlie coordination among these neurons. However, mill have been documented. Using two-electrode voltage clamp recordings, we found that graded synaptic currents (LG, IC, DG) were primarily negligible saline, but enhanced by -SIFamide. Further, entrain each other during application, indicating bidirectional, functional connectivity. Thus, mediates through parallel actions, modulating properties it as shown here, bidirectional New Noteworthy be simultaneously with separate networks. Both recruitment into, with, occur synapses. Alternatively, ionic currents. find determined modulate typically ineffective. complementary modulatory peptide actions

Language: Английский

Neural circuits underlying context-dependent competition between defensive actions inDrosophilalarva DOI Open Access

Maxime Lehman,

Chloé Barré,

Md Amit Hasan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 25, 2023

Abstract To ensure their survival, animals must be able to respond adaptively threats within environment. However, the precise neural circuit mechanisms that underlie such flexible defensive behaviors remain poorly understood. Using neuronal manipulations, machine-learning-based behavioral detection, Electron Microscopy (EM) connectomics and calcium imaging in Drosophila larva, we have mapped second-order interneurons differentially involved competition between different actions main pathways motor side putatively inhibiting startle-type promoting escape a context dependent manner. We found mechanosensory stimulation modulates nociceptive sequences inhibits C-shape bends Rolls favor of startle-like behaviors. This suggests mechanosensory-induced startle responses Structural functional connectivity revealed second order receive input from projection neurons integrate stimuli. The analysis postsynaptic EM they make indirect connections pre-motor neurons. Finally, identify pair descending could promote modulate sequence Altogether, these results characterize Startle Escape competition, modulated by sensory context.

Language: Английский

Citations

4
Two neuropeptides that promote blood-feeding inAnopheles stephensimosquitoes DOI Creative Commons
Prashali Bansal,

Roshni Pillai,

DB Pooja

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 15, 2024

Animals routinely need to make decisions about what eat and when. These are influenced not only by the availability quality of food but also internal state animal, which needs compute give weights these different variables before making a choice. Feeding preferences female mosquitoes exemplify this behavioural plasticity. Both male usually feed on carbohydrate-rich sources nectar or sap, feeds blood, is essential for egg development. This blood-appetite modulated across female’s reproductive cycle, yet little known factors that bring it about. We show Anopheles stephensi females, major vector urban Malaria Indian sub-continent West-Africa, suppress blood-feeding between blood-meal until oviposition. Transcriptomics blood-deprived sated An. mid-brains allowed us shortlist several candidate genes might promote behaviour. Through dsRNA-mediated knockdown nine them, we identified two neuropeptides – short Neuropeptide F (sNPF) RYamide blood-feeding. find neuromodulators expressed in non-overlapping subsets neurons brain suggesting behaviour likely multiple mechanisms acting neurons.

Language: Английский

Citations

1
Mechanisms of Variability Underlying Odor-Guided Locomotion DOI Creative Commons
L. Y. Tao, Vikas Bhandawat

Frontiers in Behavioral Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: May 4, 2022

Changes in locomotion mediated by odors (odor-guided locomotion) are an important mechanism which animals discover resources to their survival. Odor-guided locomotion, like most other behaviors, is highly variable. Variability behavior can arise at many nodes along the circuit that performs sensorimotor transformation. We review these sources of variability context Drosophila olfactory system. While important, using a model for we show another contributor behavioral stochastic nature decision-making during as well persistence decisions: Flies choose speed and curvature stochastically from distribution locomote with same extended periods. This stochasticity will result even if there no noise Overall, transformation amplified mechanisms making odor-guided flies

Language: Английский

Citations

6
Distinct neurogenetic mechanisms establish the same chemosensory valence state at different life stages in Caenorhabditis elegans DOI Creative Commons
Navonil Banerjee,

Elisa J. Rojas Palato,

Pei-Yin Shih

et al.

G3 Genes Genomes Genetics, Journal Year: 2023, Volume and Issue: 14(2)

Published: Nov. 23, 2023

An animal's preference for many chemosensory cues remains constant despite dramatic changes in the internal state. The mechanisms that maintain across different physiological contexts remain poorly understood. We previously showed distinct patterns of neural activity and motor output are evoked by carbon dioxide (CO2) starved adults vs dauers Caenorhabditis elegans, two life stages displaying same (attraction) CO2. However, how CO2-evoked dynamics contribute to CO2 attraction at remained unclear. Here, using a chemotaxis assay, we show interneurons employed drive stages. also investigate molecular mediate adults. insulin signaling promotes but not combinations neurotransmitters neuropeptides used Our findings provide new insight into cellular C. elegans generate attractive behavioral responses

Language: Английский

Citations

3
Neuropeptide Modulation of Bidirectional Internetwork Synapses DOI Creative Commons
Savanna-Rae H. Fahoum, Dawn M. Blitz

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 16, 2023

Abstract Oscillatory networks underlying rhythmic motor behaviors, and sensory complex neural processing, are flexible, even in their neuronal composition. Neuromodulatory inputs enable neurons to switch participation between networks, or participate multiple simultaneously. Neuromodulation of internetwork synapses can both recruit coordinate a switching neuron second network. We previously identified an example which is recruited into dual-network activity via peptidergic modulation intrinsic properties. now ask whether the same neuropeptide also modulates for coordination. The crab ( Cancer borealis ) stomatogastric nervous system contains two well-defined feeding-related (pyloric, food filtering, ∼1 Hz; gastric mill, chewing, ∼0.1 Hz). projection MCN5 uses Gly 1 -SIFamide pyloric-only LPG dual pyloric plus mill-timed bursting LPG’s Descending input not required coordinated rhythm, thus intra-network its network must underlie coordination among these neurons. However, mill have been documented. Using two-electrode voltage clamp recordings, we found that graded synaptic currents (LG, IC, DG) were primarily negligible saline, but enhanced by -SIFamide. Further, entrain each other during application, indicating bidirectional, functional connectivity. Thus, mediates through parallel actions, modulating properties it as shown here, bidirectional New Noteworthy be simultaneously with separate networks. Both recruitment into, with, occur synapses. Alternatively, ionic currents. find determined modulate typically ineffective. complementary modulatory peptide actions

Language: Английский

Citations

2