Adolescent social housing protects against adult emotional and cognitive deficits and alters the PFC and NAc transcriptome in male and female C57BL/6J mice DOI Creative Commons

Jyoti Lodha,

Emily R. Brocato,

M W Nash

et al.

Frontiers in Neuroscience, Journal Year: 2023, Volume and Issue: 17

Published: Dec. 7, 2023

Introduction Adolescence is a critical period in cognitive and emotional development, characterized by high levels of social interaction increases risk-taking behavior including binge drinking. Adolescent exposure to stress ethanol have individually been associated with the development social, emotional, deficits, as well increased risk for alcohol use disorder. Disruption cortical early life and/or drinking may partly underlie these enduring cognitive, behavioral effects. The study goal implement novel neighbor housing environment identify effects adolescent on (1) battery tasks (2) adult behavior, (3) nucleus accumbens prefrontal cortex transcriptome. Methods male female C57BL/6J mice were single or housed without access intermittent ethanol. One cohort underwent testing during adulthood determine preference, expression anxiety-like performance, patterns intake. second was sacrificed late adolescence brain tissue used transcriptomics analysis. Results As adults, displayed decreased interaction, deficits object recognition task, relative neighbor-housed mice. There no effect condition consumption. did not alter Transcriptomics analysis revealed that resulted differential genes related synaptic plasticity methylation, extracellular matrix inflammation cortex. Discussion results indicate via model protect against deficits. In addition, suggest alterations be mediated part dysregulation transcription frontal accumbens.

Language: Английский

Biotin Mitigates Alcohol Withdrawal‐Induced Anxiety and Depression by Regulating Serotonin Metabolism, BDNF, Inflammation, and Oxidative Stress in Rats DOI Creative Commons

Dawood Hossaini,

Arash Alipour, Meysam Sajjadi

et al.

Neuropsychopharmacology Reports, Journal Year: 2025, Volume and Issue: 45(1)

Published: Jan. 4, 2025

ABSTRACT Introduction Substance use disorders, particularly alcohol represent a significant public health problem, with adolescents vulnerable to their adverse effects. This study examined the possible anxiolytic and antidepressant effects of biotin, crucial vitamin for brain function, in attenuating behavioral neurobiological changes associated withdrawal adolescent rats. Materials Methods Sixty male Sprague–Dawley rats were exposed 20% ethanol solution 21 days, followed by 21‐day drug‐free period assess long‐term physiological changes. Behavioral assessments included Open Field Test, Elevated Plus Maze, Forced Swimming administered post‐withdrawal evaluate anxiety depression behaviors. Additionally, biochemical analyses performed measure serotonin levels, monoamine oxidase‐A (MAO‐A) activity, BDNF concentrations. Results The results indicate that significantly induced anxiety‐ depression‐like behavior However, treatment at higher doses, effectively attenuated these withdrawal‐related Mechanistically, biotin administration was found regulate oxidase brain‐derived neurotrophic factor, glial fibrillary acidic protein, alleviate oxidative stress markers cortical tissue. Discussion this suggest may have therapeutic potential alleviating negative withdrawal, those related depression. Further research is needed elucidate underlying mechanisms examine clinical supplementation individuals undergoing withdrawal.

Language: Английский

Citations

1
Voluntary adolescent alcohol exposure does not increase adulthood consumption of alcohol in multiple mouse and rat models DOI Creative Commons
Avery R. Sicher,

Andrea Liss,

Valentina Vozella

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 1, 2024

Adolescence is a period of increased risk taking, including alcohol and drug use. Multiple clinical studies report positive relationship between adolescent consumption developing an use disorder (AUD) in adulthood. However, few preclinical have attempted to tease apart the biological contributions exposure, independent other social, environmental, stress factors, that been conducted show mixed results. Here we several voluntary models, across four labs three institutes with two rodent species, investigate ramifications on adulthood controlled, pre-clinical environments. We consistently demonstrate lack robust increases consumption. This work highlights risks seen both human datasets murine drinking models may be due unique social environmental factors - some which humans.

Language: Английский

Citations

4
Sexually dimorphic effects of a modified adolescent social isolation paradigm on behavioral risk factors of alcohol use disorder in Long Evans Rats DOI Creative Commons
Olivia A. Ortelli, Stacy R. Pitcairn,

Christina Dyson

et al.

Addiction Neuroscience, Journal Year: 2023, Volume and Issue: 9, P. 100134 - 100134

Published: Nov. 4, 2023

Early life stress (ELS) is a major risk factor for alcohol use disorder (AUD) and comorbid neuropsychiatric conditions. We previously demonstrated that an adolescent social isolation (aSI) model of ELS significantly increased behavioral factors these disorders (e.g. anxiety-like behaviors, drinking) in male, but not female rats. Since many neurodevelopmental milestones are accelerated females, we investigated whether earlier/shorter window (PND 21-38) would yield comparable phenotypes both sexes. In two experiments, Long Evans rats were socially isolated (SI) or group-housed (GH) on postnatal day (PND) 21 locomotion was assessed the open field test (OFT; PND 30). Experiment 1 also behavior elevated plus-maze (EPM) 32). 2, all single housed 38 to assess home cage drinking. revealed SI females had locomotor activity OFT did differ from GH subjects EPM. The results replicated sexes 2 male greater ethanol consumption during eight continuous access paradigm. contrast, subsequent intermittent two-bottle choice drinking, only displayed intake preference quinine-adulterated solution. These findings demonstrate early can promote AUD vulnerability-related there profound sex differences vulnerability this stressor. Uncovering neural mechanisms responsible sexually dimorphic sensitivity may shed light biological substrates associated with negative emotion men women.

Language: Английский

Citations

10
Adolescent Alcohol Exposure Dysregulates Developing Cortical GABA Circuits DOI
Avery R. Sicher, Nicole A. Crowley

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown, P. 159 - 177

Published: Jan. 1, 2025

Language: Английский

Citations

0
Targeting Persistent Changes in Neuroimmune and Epigenetic Signaling in Adolescent Drinking to Treat Alcohol Use Disorder in Adulthood DOI Creative Commons
Fulton T. Crews, Leon G. Coleman, Victoria A. Macht

et al.

Pharmacological Reviews, Journal Year: 2022, Volume and Issue: 75(2), P. 380 - 396

Published: Dec. 12, 2022

Studies universally find early age of drinking onset is linked to lifelong risks alcohol problems and use disorder (AUD). Assessment the lasting effect during adolescent development in humans confounded by diversity environmental genetic factors that affect development, including emerging personality disorders progressive increases trajectories into adulthood. Preclinical studies using an intermittent ethanol (AIE) exposure rat model underage binge avoid human confounds support changes increase risks. AIE adult drinking, risky decision-making, reward-seeking, anxiety as well reductions executive function all for AUD. causes persistent brain neuroimmune signaling high-mobility group box 1 (HMGB1), Toll-like receptor, receptor advanced glycation end products, innate immune genes are also found be increased AUD brain. HMGB1 released from cells ethanol, both free within extracellular vesicles, act on neurons glia, shifting transcription cellular phenotype. AIE-induced decreases hippocampal neurogenesis loss basal forebrain cholinergic reviewed examples pathology. Both prevented reversed anti-inflammatory epigenetic drugs. Findings suggest AIE-increased induces RE-1 silencing transcript blunting gene expression, neuronal Inhibition signaling, histone methylation enzymes, galantamine, cholinesterase inhibitor, prevent reverse These findings provide new targets may neuropathology other diseases signaling.

Significance Statement

Adolescent earlier associated with high levels lifetime adults developing problems. Loss how novel therapeutic

Language: Английский

Citations

16
Voluntary adolescent alcohol exposure does not robustly increase adulthood consumption of alcohol in multiple mouse and rat models DOI Creative Commons
Avery R. Sicher,

Andrea Liss,

Valentina Vozella

et al.

Addiction Neuroscience, Journal Year: 2024, Volume and Issue: 12, P. 100171 - 100171

Published: Aug. 3, 2024

Adolescence is a period of increased risk taking, including alcohol and drug use. Multiple clinical studies report positive relationship between adolescent consumption developing an use disorder (AUD) in adulthood. However, few preclinical have attempted to tease apart the biological contributions exposure, independent other social, environmental, stress factors, that been conducted show mixed results. Here we several voluntary models, across four labs three institutes with two rodent species, investigate ramifications on adulthood controlled, pre-clinical environments. We consistently demonstrate lack robust increases consumption. This work highlights risks seen both human datasets murine drinking models may be due unique social environmental factors - some which humans.

Language: Английский

Citations

2
Adolescent social isolation increases binge-like alcohol drinking in male but not female high-alcohol-preferring mice DOI

Eva Cullins,

Julia A. Chester

Alcohol and Alcoholism, Journal Year: 2024, Volume and Issue: 59(2)

Published: Jan. 17, 2024

Abstract Aims This study examined how adolescent social isolation affects adult binge-like alcohol drinking and stress–axis function, via basal levels of circulating corticosterone (CORT), in male female mice with a genetic predisposition toward high preference (HAP). Methods Male HAP2 were randomly assigned to group-housed or (ISO) group. Social began at postnatal Days 40–42 lasted for 21 days prior assessment using 4-day drinking-in-the-dark (DID) procedure. Blood samples assess CORT taken 6 after ended 24 h before DID started, again 60 ended, during the light portion cycle. Results Adolescent increased but not mice. All groups showed significantly lower compared DID. Pearson bivariate correlation coefficients between first 2 grams-per-kilogram intake on Day 4 indicated significant positive ISO males only negative correlations females Conclusions These findings demonstrate that Stress–axis adaptations may be associated social-isolation-induced increase drinking.

Language: Английский

Citations

1
Associations between binge drinking experience, depressive mood, and suicidality in adolescents: Based on the 2021 Korea Youth Risk Behavior Web-based Survey DOI Creative Commons
Mi‐Sun Lee, Hoo-Yeon Lee

Journal of Affective Disorders, Journal Year: 2022, Volume and Issue: 323, P. 386 - 391

Published: Nov. 30, 2022

This study aimed to investigate the associations between binge drinking experience (BDE), depressive mood, and suicidality among Korean adolescents. We analyzed 17th population-based cross-sectional data from Youth Risk Behavior Web-based Survey. Multiple logistic regression analysis was performed compare BDE, by sex. In 54,848 adolescents, prevalence of BDE 5.3 % in boys 4.4 girls. The suicidal ideation, plans, attempts girls 7.8 %, 2.4 1.4 respectively. suicide with both moods higher than that boys. Boys mood had 12.60 times 14.50 adjusted odds ratio (aOR) ideation plan, respectively, compared non-drinking/non-depressed group. Girls 22.06 aOR has few limitations including use self-report questionnaires, participants' response bias, lack diagnosis mental health professionals. These findings represent South adolescents or mood. Since showed particularly high suicidality, it is necessary establish services, such as proper prevention strategies targeted interventions.

Language: Английский

Citations

6
Adolescent social housing protects against adult emotional and cognitive deficits and alters the PFC and NAc transcriptome in male and female C57BL/6J mice DOI Creative Commons

Jyoti Lodha,

Emily R. Brocato,

M W Nash

et al.

Frontiers in Neuroscience, Journal Year: 2023, Volume and Issue: 17

Published: Dec. 7, 2023

Introduction Adolescence is a critical period in cognitive and emotional development, characterized by high levels of social interaction increases risk-taking behavior including binge drinking. Adolescent exposure to stress ethanol have individually been associated with the development social, emotional, deficits, as well increased risk for alcohol use disorder. Disruption cortical early life and/or drinking may partly underlie these enduring cognitive, behavioral effects. The study goal implement novel neighbor housing environment identify effects adolescent on (1) battery tasks (2) adult behavior, (3) nucleus accumbens prefrontal cortex transcriptome. Methods male female C57BL/6J mice were single or housed without access intermittent ethanol. One cohort underwent testing during adulthood determine preference, expression anxiety-like performance, patterns intake. second was sacrificed late adolescence brain tissue used transcriptomics analysis. Results As adults, displayed decreased interaction, deficits object recognition task, relative neighbor-housed mice. There no effect condition consumption. did not alter Transcriptomics analysis revealed that resulted differential genes related synaptic plasticity methylation, extracellular matrix inflammation cortex. Discussion results indicate via model protect against deficits. In addition, suggest alterations be mediated part dysregulation transcription frontal accumbens.

Language: Английский

Citations

1