The effects of locus coeruleus ablation on mouse brain volume and microstructure evaluated by high-field MRI DOI Creative Commons
Rasmus West Knopper, Christian Stald Skoven, Simon F. Eskildsen

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Dec. 11, 2024

The locus coeruleus (LC) produces most of the brain's noradrenaline (NA). Among its many roles, NA is often said to be neuroprotective and important for brain upkeep. For this reason, loss LC integrity thought impact volume microstructure as well plasticity broadly. dysfunction also a suspected driver in development neurodegenerative diseases. Nevertheless, on gross structure normal brains not well-studied. We employed high-field ex vivo magnetic resonance imaging (MRI) investigate volumetrics control (CON) mice with ablation (LCA) at two ages, representing developing fully matured brain. These whole-brain methods are known capable detecting subtle morphological changes microstructural remodeling. found behavior consistent histologically confirmed ablation. However, MRI showed no difference between CON LCA groups regard size, relative regional volumes, or indices. Our findings suggest that LC-NA needed postnatal maturation growth mice. Nor it required maintenance adult mouse brain, atrophy aberration detected after weeks dysfunction. This adds clarity often-encountered notion "trophic support" shows such effects likely relevant mechanisms related neuroprotection (pre)diseased

Language: Английский

Damage to the Locus Coeruleus Alters the Expression of Key Proteins in Limbic Neurodegeneration DOI Open Access
Francesca Biagioni, Michela Ferrucci, Gloria Lazzeri

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3159 - 3159

Published: March 9, 2024

The present investigation was designed based on the evidence that, in neurodegenerative disorders, such as Alzheimer’s dementia (AD) and Parkinson’s disease (PD), damage to locus coeruleus (LC) arising norepinephrine (NE) axons (LC-NE) is documented hypothesized foster onset progression of neurodegeneration within target regions. Specifically, experiments were assess whether selective LC-NE may alter key proteins involved specific limbic regions, hippocampus piriform cortex, compared with dorsal striatum. To achieve this, a loss induced by neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) C57 Black mice, assessed NE dopamine-beta-hydroxylase In these experimental conditions, amount alpha-synuclein (alpha-syn) protein levels increased along alpha-syn expressing neurons cortex. Similar findings obtained concerning phospho-Tau immunoblotting. contrast, decrease inducible HSP70-expressing sequestosome (p62)-expressing cells, at immunoblotting, reported. data provide further understand why AD engagement during PD.

Language: Английский

Citations

3

The effects of locus coeruleus ablation on mouse brain volume and microstructure evaluated by high-field MRI DOI Creative Commons
Rasmus West Knopper, Christian Stald Skoven, Simon F. Eskildsen

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Dec. 11, 2024

The locus coeruleus (LC) produces most of the brain's noradrenaline (NA). Among its many roles, NA is often said to be neuroprotective and important for brain upkeep. For this reason, loss LC integrity thought impact volume microstructure as well plasticity broadly. dysfunction also a suspected driver in development neurodegenerative diseases. Nevertheless, on gross structure normal brains not well-studied. We employed high-field ex vivo magnetic resonance imaging (MRI) investigate volumetrics control (CON) mice with ablation (LCA) at two ages, representing developing fully matured brain. These whole-brain methods are known capable detecting subtle morphological changes microstructural remodeling. found behavior consistent histologically confirmed ablation. However, MRI showed no difference between CON LCA groups regard size, relative regional volumes, or indices. Our findings suggest that LC-NA needed postnatal maturation growth mice. Nor it required maintenance adult mouse brain, atrophy aberration detected after weeks dysfunction. This adds clarity often-encountered notion "trophic support" shows such effects likely relevant mechanisms related neuroprotection (pre)diseased

Language: Английский

Citations

1