Satb2 and Nr4a2 are required for the differentiation of cortical layer 6b DOI Creative Commons
Ning‐Ning Song, Li Zhao,

Yun‐Chao Tao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 13, 2024

Abstract Cortical layer 6 is divided into two sublayers, and 6b situated above the white matter with distinct architecture from 6a. Layer arises subplate contains earliest born neurons in development of cerebral cortex. Although great progress has been made understanding cortical morphogenesis, there a dearth knowledge regarding molecular mechanisms governing neurons. Here we report that transcription factor special AT-rich binding protein 2 (Satb2) nuclear receptor subfamily 4 group A member (Nr4a2) are required for normal differentiation Upon conditional deletion Satb2 cortex (Satb2Emx1 CKO) or selectively inactivation only (Satb2Nr4a2CreER CKO), expressions 6b-specific genes (i.e., Ctgf, Cplx3, Trh Tnmd) were significantly reduced, whereas Nr4a2 was dramatically increased, underscoring involved cell-autonomous manner. On other hand, when deleted cortex, Tnmd upregulated unchanged expression Ctgf Cplx3. Notably, defective resulting remained Satb2/Nr4a2 double CKO mice. In summary, our findings indicated both possibly via different pathways.

Language: Английский

Layer 6b controls brain state via apical dendrites and the higher-order thalamocortical system DOI Creative Commons
Timothy A. Zolnik,

Anna Bronec,

Annemarie D. Ross

et al.

Neuron, Journal Year: 2023, Volume and Issue: 112(5), P. 805 - 820.e4

Published: Dec. 14, 2023

Language: Английский

Citations

22
Spatial Single-cell Analysis Decodes Cortical Layer and Area Specification DOI Creative Commons
Xuyu Qian, Kyle Coleman,

Shunzhou Jiang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 5, 2024

The human cerebral cortex, pivotal for advanced cognitive functions, is composed of six distinct layers and dozens functionally specialized areas

Language: Английский

Citations

6
Layer 1 neocortex: Gating and integrating multidimensional signals DOI Creative Commons
Shuhan Huang, Jingjing Sherry Wu, Giulia Sansone

et al.

Neuron, Journal Year: 2023, Volume and Issue: 112(2), P. 184 - 200

Published: Oct. 31, 2023

Language: Английский

Citations

14
Satb2 and Nr4a2 are required for the differentiation of cortical layer 6b DOI Creative Commons
Li Zhao,

Yun‐Chao Tao,

Ling Hu

et al.

Cell Death Discovery, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 31, 2025

Language: Английский

Citations

0
Chronic silencing of Drd1a-Cre+ neurons impairs dopaminergic-driven cortical activation DOI Creative Commons
Fernando Messore, Augustė Vadišiūtė,

Hayley Edmead

et al.

Frontiers in Neuroanatomy, Journal Year: 2025, Volume and Issue: 19

Published: April 28, 2025

In the somatosensory cortex of transgenic mice, Cre-recombinase is expressed under control dopamine receptor D1 (Drd1a) promoter in lower layer 6. These neurons selectively project to higher-order thalamic nuclei and participate cortico-thalamo-cortical loops involved sensory processing stimulus representation. However, role dopaminergic modulation activating this neuronal population during cortical arousal remains poorly understood. study, we examined effects (SKF-81297) D2 (Quinpirole) agonists on network activation. We further investigated consequences silencing these using a Snap25 conditional knockout mouse model. report decrease cellular density subplate/L6b with normal development from P8 adulthood. Conversely, Drd1a-Cre+ goes up cKO brains when comparing same ages. Moreover, observe that has no effect microglial cells. Our results demonstrate both require modulate activity effectively. study provides new insights into fundamental activation processing.

Language: Английский

Citations

0
Distinct distribution of subplate neuron subtypes between the sensory cortices during the early postnatal period DOI
Minzi Chang,

Sophia Nehs,

Zheng Xu

et al.

The Journal of Comparative Neurology, Journal Year: 2024, Volume and Issue: 532(2)

Published: Feb. 1, 2024

Abstract Subplate neurons (SpNs) are a heterogeneous neuronal population actively involved in early cortical circuit formation. In rodents, many SpNs survive and form layer 6b. The molecular heterogeneity of raises the question whether different subpopulations through postnatal period similarly such diverse SpN populations auditory cortex (ACtx) share common distribution pattern with other sensory systems. To address that, we investigated expression multiple specific markers ACtx, as well visual (VCtx) somatosensory (SCtx) cortices controls, using complexin 3 (Cplx3) antibodies SpN‐specific Cre‐driver mice, connective tissue growth factor ( CTGF ), dopamine receptor D1 Drd1a neurexophilin 4 Nxph4 ). We focused on two time windows development: (1) during second week (PNW) before ear‐canal opening (2) third PNW after opening. compared ACtx VCtx SCtx. At both examined timepoints, Cplx3 expressing largest smallest respectively. Similar patterns observable SCtx but not PNW, for higher proportion is detected This study suggests that might contribute differently to development individual circuits.

Language: Английский

Citations

2
Correlation between the number of interstitial neurons of the white matter and number of neurons within cortical layers: Histological analyses in postnatal macaque DOI Creative Commons
Bashir Ahmed, Alvaro Duque, Pasko Rakić

et al.

The Journal of Comparative Neurology, Journal Year: 2024, Volume and Issue: 532(6)

Published: June 1, 2024

Abstract We have examined the number and distribution of NeuN‐immunoreactive cortical white matter interstitial cells (WMICs) compared them to neurons in layers 1–6 across overlying cortex coronal sections from postnatal macaques. The data been gathered over 300 selected regions at gyral crowns, sulci, linear where we also determined layer thicknesses: standard thicknesses tangential thicknesses. Cortical cell numbers showed variability according gyral, linear, or sulcal regions. In spite these variations, our standardized 1 6b underlying 6b‐white boundary shown a consistent correlation between WMICs 5 6a on all studied: for each WMIC, there are order five approximately three 6a, irrespective origins area whether they propose that macaque is correlated density within and, clinical perspective, change schizophrenia epilepsy may fact be linked neurons.

Language: Английский

Citations

2
Hidden in the white matter: Current views on interstitial white matter neurons DOI Creative Commons
Maximilian Fischer, Maria Kukley

The Neuroscientist, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 4, 2024

The mammalian brain comprises two structurally and functionally distinct compartments: the gray matter (GM) white (WM). In humans, WM constitutes approximately half of volume, yet it remains significantly less investigated than GM. major cellular elements are neuronal axons glial cells. However, also contains cell bodies interstitial neurons, estimated to number 10 28 million in adult bat brain, 67 Lar gibbon 450 670 human representing as much 1.3%, 2.25%, 3.5% all neurons cerebral cortex, respectively. Many studies (IWMNs) using immunohistochemistry, some information is available regarding their electrophysiological properties. functional role IWMNs physiologic pathologic conditions largely unknown. This review aims provide a concise update distribution properties highlight possible functions these cells debated literature, speculate about other interactions with We hope that our will inspire new research on IWMNs, which represent an intriguing population brain.

Language: Английский

Citations

2
Satb2 and Nr4a2 are required for the differentiation of cortical layer 6b DOI Creative Commons
Ning‐Ning Song, Li Zhao,

Yun‐Chao Tao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 13, 2024

Abstract Cortical layer 6 is divided into two sublayers, and 6b situated above the white matter with distinct architecture from 6a. Layer arises subplate contains earliest born neurons in development of cerebral cortex. Although great progress has been made understanding cortical morphogenesis, there a dearth knowledge regarding molecular mechanisms governing neurons. Here we report that transcription factor special AT-rich binding protein 2 (Satb2) nuclear receptor subfamily 4 group A member (Nr4a2) are required for normal differentiation Upon conditional deletion Satb2 cortex (Satb2Emx1 CKO) or selectively inactivation only (Satb2Nr4a2CreER CKO), expressions 6b-specific genes (i.e., Ctgf, Cplx3, Trh Tnmd) were significantly reduced, whereas Nr4a2 was dramatically increased, underscoring involved cell-autonomous manner. On other hand, when deleted cortex, Tnmd upregulated unchanged expression Ctgf Cplx3. Notably, defective resulting remained Satb2/Nr4a2 double CKO mice. In summary, our findings indicated both possibly via different pathways.

Language: Английский

Citations

0