AAV-mediated transduction of songbird retina DOI Creative Commons
Pranav Kumar Seth, Dominik Heyers,

Baladev Satish

et al.

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

Introduction Genetic manipulation of murine retinal tissue through ocular administration adeno-associated viruses (AAVs) has become a standard technique to investigate multitude mechanisms underlying physiology. Resultantly, developments recombinant viral vectors with improved transduction efficiency and further methodological improvements have mostly focused on tissue, whereas AAVs successfully targeting avian retinae remained scarce. Methodology Using custom-designed injection setup, we identified serotype the capability induce widespread bird retina. Results Intravitreal an AAV type 2/9 encoding for enhanced green fluorescent protein (EGFP) in night-migratory European robins ( Erithacus rubecula ) resulted coverages up 60% within tissue. Subsequent immunohistochemical analyses revealed that AAV2/9-EGFP almost exclusively targeted photoreceptors: rods, various single cones (UV, blue, green, red cones), both (accessory principal) members double cones. Discussion The consistently high photoreceptor-specific makes AAV2/9 powerful tool carrying out genetic manipulations photoreceptors, thus opening wealth opportunities physiological aspects processing birds, such as recordings and/or post-transductional behavioural readouts future vision-related research.

Language: Английский

Comparative transcriptomic insights into the evolution of vertebrate photoreceptor types DOI Creative Commons
Dario Tommasini, Takeshi Yoshimatsu, Teresa Puthussery

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

2

A standardized nomenclature for the rods and cones of the vertebrate retina DOI Creative Commons
Tom Baden, Juan M Angueyra, Jenny M. Bosten

et al.

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(5), P. e3003157 - e3003157

Published: May 7, 2025

Vertebrate photoreceptors have been studied for well over a century, but fixed nomenclature referring to orthologous cell types across diverse species has lacking. Instead, variably—and often confusingly—named according morphology, presence/absence of ‘rhodopsin’, spectral sensitivity, chromophore usage, and/or the gene family opsin(s) they express. Here, we propose unified vertebrate rods and cones that aligns with naming systems other retinal classes is based on photoreceptor type’s putative evolutionary history. This classification informed by functional, anatomical, developmental, molecular identities neuron as whole, including expression deeply conserved transcription factors required development. The proposed names will be applicable all vertebrates indicative widest possible range properties, their postsynaptic wiring, hence allude common species-specific roles in vision. Furthermore, system open-ended accommodate future discovery as-yet unknown types.

Language: Английский

Citations

2

AAV-mediated transduction of songbird retina DOI Creative Commons
Pranav Kumar Seth, Dominik Heyers,

Baladev Satish

et al.

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

Introduction Genetic manipulation of murine retinal tissue through ocular administration adeno-associated viruses (AAVs) has become a standard technique to investigate multitude mechanisms underlying physiology. Resultantly, developments recombinant viral vectors with improved transduction efficiency and further methodological improvements have mostly focused on tissue, whereas AAVs successfully targeting avian retinae remained scarce. Methodology Using custom-designed injection setup, we identified serotype the capability induce widespread bird retina. Results Intravitreal an AAV type 2/9 encoding for enhanced green fluorescent protein (EGFP) in night-migratory European robins ( Erithacus rubecula ) resulted coverages up 60% within tissue. Subsequent immunohistochemical analyses revealed that AAV2/9-EGFP almost exclusively targeted photoreceptors: rods, various single cones (UV, blue, green, red cones), both (accessory principal) members double cones. Discussion The consistently high photoreceptor-specific makes AAV2/9 powerful tool carrying out genetic manipulations photoreceptors, thus opening wealth opportunities physiological aspects processing birds, such as recordings and/or post-transductional behavioural readouts future vision-related research.

Language: Английский

Citations

0