Whole-brain imaging of freely-moving zebrafish

Frontiers in Neuroscience, Journal Year: 2023, Volume and Issue: 17

Published: April 17, 2023

One of the holy grails neuroscience is to record activity every neuron in brain while an animal moves freely and performs complex behavioral tasks. While important steps forward have been taken recently large-scale neural recording rodent models, single resolution across entire mammalian remains elusive. In contrast larval zebrafish offers great promise this regard. Zebrafish are a vertebrate model with substantial homology brain, but their transparency allows whole-brain recordings genetically-encoded fluorescent indicators at single-neuron using optical microscopy techniques. Furthermore begin show repertoire natural behavior from early age, including hunting small, fast-moving prey visual cues. Until work address bases these behaviors mostly relied on assays where fish was immobilized under microscope objective, stimuli such as were presented virtually. However significant progress has made developing imaging techniques for which not immobilized. Here we discuss recent advances, focusing particularly based light-field microscopy. We also draw attention several outstanding issues remain be addressed increase ecological validity results obtained.

Language: Английский

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The Visual Systems of Zebrafish

Annual Review of Neuroscience, Journal Year: 2024, Volume and Issue: 47(1), P. 255 - 276

Published: April 25, 2024

The zebrafish visual system has become a paradigmatic preparation for behavioral and systems neuroscience. Around 40 types of retinal ganglion cells (RGCs) serve as matched filters stimulus features, including light, optic flow, prey, objects on collision course. RGCs distribute their signals via axon collaterals to 12 retinorecipient areas in forebrain midbrain. major visuomotor hub, the tectum, harbors nine RGC input layers that combine information multiple features. retinotopic map tectum is locally adapted scene statistics subfield–specific demands. Tectal projections premotor centers are topographically organized according commands. known connectivity more than 20 processing streams allows us dissect cellular basis elementary perceptual cognitive functions. Visually evoked responses, such prey capture or loom avoidance, controlled by dedicated multistation pathways that—at least larva—resemble labeled lines. This architecture serves neuronal code's purpose driving adaptive behavior.

Language: Английский

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Degenerate Neuronal and Circuit Mechanisms Important for Generating Rhythmic Motor Patterns

Physiological Reviews, Journal Year: 2024, Volume and Issue: 105(1), P. 95 - 135

Published: June 13, 2024

In 1996, we published a review article (Marder E, Calabrese RL.

Language: Английский

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Exploring sesquiterpene lactone as a dual therapeutic agent for diabetes and oxidative stress: insights into PI3K/AKT modulation

3 Biotech, Journal Year: 2024, Volume and Issue: 14(9)

Published: Aug. 19, 2024

Language: Английский

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Diverse prey capture strategies in teleost larvae

Published: Feb. 10, 2025

Animal behavior is adapted to the sensory environment in which it evolved, while also being constrained by physical limits, evolutionary history, and developmental trajectories. The hunting of larval zebrafish ( Danio rerio ), a cyprinid native streams Eastern India, has been well characterized. However, unknown if complement sequence movements employed during prey capture universal across freshwater teleosts. Here, we explore syntax fish belonging clade Percomorpha , whose last common ancestor with cyprinids lived ∼240 million years ago. We compared four cichlid species from Lake Tanganyika endemic deep benthic parts lake Lepidiolamprologus attenuatus Lamprologus ocellatus Neolamprologus multifasciatus ) or inhabiting rivers Astatotilapia burtoni that medaka Oryzias latipes found rice paddies East Asia. Using high speed videography neural networks, tracked eye extracted swim kinematics larvae these five species. Notably, repertoire cichlids broader than zebrafish, but shares basic features, such as convergence, positioning centrally binocular visual field, discrete bouts, including two kinds strikes. In contrast, continuously, track monocularly without position laterally before capturing them side swing. This configuration kinematic motifs suggests may judge distance predominantly motion parallax, mainly use cues. Together, our study documents diversification locomotor oculomotor adaptations among teleost larvae.

Language: Английский

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Cholecystokinin neurons in the spinal trigeminal nucleus interpolaris regulate mechanically evoked predatory hunting in male mice

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 14, 2025

Predatory hunting plays a critical role in animal survival. Motion-related vibrissal somatosensory signaling is essential for prey detection and mice. However, little known about the neural circuits that convert cues to trigger predatory hunting. Here, we report mechanical force onto area of male mice key stimulus Mechanically evoked was abrogated by chemogenetic inactivation cholecystokinin-positive (Cck+) neurons spinal trigeminal nucleus interpolaris (Sp5I). The Cck+ Sp5I responded intensity sent signals superior colliculus were relevant stereotypical motor actions. Synaptic projections from impaired mechanically attacks. Together, these data reveal circuit specifically engaged translating provoke This study reveals respond vibrissae stimuli send inputs colliculus, which are behaviour.

Language: Английский

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Diverse prey capture strategies in teleost larvae

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 4, 2023

Summary Animal behavior is adapted to the sensory environment in which it evolved, while also being constrained by physical limits, evolutionary history, and developmental trajectories. The hunting of larval zebrafish ( Danio rerio ), a cyprinid native streams Eastern India, has been well characterized. However, unknown if complement sequence movements employed during prey capture universal across freshwater teleosts. Here, we explore syntax fish belonging clade Percomorpha , whose last common ancestor with cyprinids lived ∼240 million years ago. We compared four cichlid species from Lake Tanganyika endemic deep benthic parts lake Lepidiolamprologus attenuatus Lamprologus ocellatus Neolamprologus multifasciatus ) or inhabiting rivers Astatotilapia burtoni that medaka Oryzias latipes found rice paddies East Asia. Using high speed videography neural networks, tracked eye extracted swim kinematics larvae these five species. Notably, repertoire cichlids broader than zebrafish, but shares basic features, such as convergence, positioning centrally binocular visual field, discrete bouts, including two kinds strikes. In contrast, continuously, track monocularly without position laterally before capturing them side swing. This configuration kinematic motifs suggests may judge distance predominantly motion parallax, mainly use cues. Together, our study documents diversification locomotor oculomotor adaptations among teleost larvae.

Language: Английский

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Social Human Collective Decision-Making and Its Applications with Brain Network Models

Modeling and simulation in science, engineering & technology, Journal Year: 2023, Volume and Issue: unknown, P. 103 - 141

Published: Jan. 1, 2023

A better understanding of social human dynamics would be a powerful tool to improve nearly any computational endeavour that involves interactions. This includes intelligent environments featuring, for instance, efficient illumination systems, smart evacuation signalling transportation crowd control, or disaster response. Moreover, given the population has significantly grown up in number and spread across planet, capacity predict behaviours will help demonstrate special behavioural forms observed when masses people gather together make crowds. Additionally, are characterized by complex psychological sociobiological behaviour. The contributions factors need accounted obtain more reliable models. Many models have been proposed describe group different scenarios. However, due complexity such systems amplified above factors, decision-making with multiple choices not fully scrutinized. In this chapter, we consider probabilistic drift-diffusion Bayesian inference frameworks address issue, assisting decision-making. We provide details models, as well representative numerical examples, discuss process example escape route phenomena further developing frameworks. latter context, also give review recent developments collective its applications brain network Furthermore, illustrative examples role neuromodulation, reinforcement learning processes. Finally, call attention existing challenges, open problems, promising approaches studying decision-making, including those arising from nonequilibrium considerations associated

Language: Английский

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Diverse prey capture strategies in teleost larvae

Published: July 22, 2024

Animal behavior is adapted to the sensory environment in which it evolved, while also being constrained by physical limits, evolutionary history, and developmental trajectories. The hunting of larval zebrafish ( Danio rerio ), a cyprinid native streams Eastern India, has been well characterized. However, unknown if complement sequence movements employed during prey capture universal across freshwater teleosts. Here, we explore syntax fish belonging clade Percomorpha , whose last common ancestor with cyprinids lived ∼240 million years ago. We compared four cichlid species endemic deep benthic parts Lake Tanganyika Lepidiolamprologus attenuatus Lamprologus ocellatus Neolamprologus multifasciatus ) or inhabiting rivers around that same lake Astatotilapia burtoni medaka Oryzias latipes found rice paddies East Asia. Using high speed videography neural networks, tracked eye extracted swim kinematics from these five species. Notably, repertoire cichlids broader than zebrafish, but shares basic features, such as convergence, positioning centrally binocular visual field, discrete bouts, including two kinds strikes. In contrast, continuously, track monocularly without position laterally before capturing them side swing. This configuration kinematic motifs suggests may judge distance motion parallax, use cues. Together, our study documents diversification locomotor oculomotor adaptations among teleost larvae.

Language: Английский

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How do fish miss? Attack strategies of threespine stickleback capturing non-evasive prey

Journal of Experimental Biology, Journal Year: 2024, Volume and Issue: 227(22)

Published: Oct. 6, 2024

Most predators rely on capturing prey for survival, yet failure is common. Failure often attributed to evasion, but predator miscalculation and/or inaccuracy may also drive an unsuccessful event. We addressed the latter using threespine stickleback as and bloodworms (non-evasive) prey. High-speed videography of entire attack allowed us determine strike tactics leading successful or missed strikes. analyzed movements morphological traits from 57 individuals. Our results reveal that kinematics outcome failed strikes primarily arise incorrect timing mouth opening, beginning too far suction be effective. This likely stems lack integration between locomotion feeding systems. study begins unravel important link behavior success in fish feeding.

Language: Английский

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